LOCUS       sequence-84376-e        1675 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1675)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Pit1-6
FEATURES             Location/Qualifiers
     source          1..1675
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      911..920
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(917..919,920..922,923..1636)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(962..983)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1223..1242)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1570..1591
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagccg ccaccatgag ttgccaacct ttcacctcgg ctgatacctt
       61 tatacctctg aattctgacg cttctgctgc cctgcctctg agaatgcacc acagtgccgc
      121 tgagggtctc ccagcctcca accacgccac caacgtgatg tccacagcga caggacttca
      181 ttattctgtg ccttcctgtc attatggaaa ccagccatcc acctacggag tgatggcagg
      241 cactttaacc ccttgtcttt acaagtttcc agaccacacc ctgagtcatg ggtttcctcc
      301 cctgcaccaa cctctgctgg cggaggaccc aacagcctct gaatttaagc aggaactcag
      361 gcggaaaagt aaattggtgg aagagccaat agacatggac tccccggaaa tccgagaact
      421 ggagcagttt gccaacgaat ttaaagtgag aagaattaag ttaggataca cccagacaaa
      481 cgtgggcgaa gctctggccg ctgtccacgg ctccgaattc agtcaaacaa ccatctgccg
      541 atttgaaaac ttgcagctca gtttcaaaaa tgcttgcaaa ctgaaagcaa ttttatccaa
      601 gtggctggag gaagctgagc aggtcggagc tttgtacaat gaaaaagtgg gagcaaacga
      661 aaggaagagg aaacggagga caactatcag tatcgccgct aaggatgctt tggagagaca
      721 ctttggagag cacagcaaac cttcttcgca ggagatcatg cggatggctg aagaattgaa
      781 tctcgagaaa gaagtagtaa gagtgtggtt ttgcaaccga aggcagagag aaaaacgggt
      841 gaaaacgagt ctcaatcaaa gtctgttctc tatttcaaag gagcatcttg agtgcagaga
      901 tccaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
      961 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     1021 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     1081 cgtgccctgg cccaccctcg tgaccacctt gacctacggc gtgcagtgct tcgcccgcta
     1141 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     1201 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     1261 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     1321 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaaggtct atatcaccgc
     1381 cgacaagcag aagaacggca tcaaggtgaa cttcaagacc cgccacaaca tcgaggacgg
     1441 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     1501 gctgcccgac aaccactacc tgagcaccca gtccaagctg agcaaagacc ccaacgagaa
     1561 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     1621 cgagctgtac aagtaagcgg ccgcgactct agatcataat cagccatacc acatt
//
