LOCUS       sequence-84378-e        2687 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Fimbrin/Cytoskeleton, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2687)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Plastin-N-10
FEATURES             Location/Qualifiers
     source          1..2687
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      14..23
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     RBS             1354..1362
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     regulatory      1931..1940
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1937..1939,1940..1942,1943..2656)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1982..2003)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2243..2262)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2590..2611
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atccgctagc accgccacca tggaaaacag tactactacc atttctcggg aggagcttga
       61 agaactacaa gaggcattta ataaaataga tattgacaat agtgggtatg tcagtgacta
      121 tgaacttcaa gacctgttta aggaagcaag ccttcctctg cctggctaca aggtgcgcga
      181 gattgtggag aaaattctat cagttgctga cagcaacaaa gatggcaaaa tcagttttga
      241 agagtttgtg tcactaatgc aagaattaaa aagcaaagat atcagcaaaa cattccgaaa
      301 aataattaac aagagggaag ggattactgc tattggagga acttcaacta tttccagtga
      361 gggcacacag cattcttatt cagaggaaga aaaagtggct tttgttaact ggataaacaa
      421 agccctggag aatgaccctg actgtaagca tcttataccc atgaatccca atgatgatag
      481 tcttttcaag tcacttgcag atggcatcct tctttgcaaa atgatcaact tatctgaacc
      541 agatacaatt gatgaaagag ccatcaataa gaaaaagctc acgccattca ctatttctga
      601 aaatttaaac ctagctctga attctgcctc agccattggt tgtacagtgg tcaacattgg
      661 tgcatcagat ctcaaagaag gaaaacctca cttggtcttg ggacttctct ggcagatcat
      721 caaagttggc ctttttgctg atattgagat ttccaggaat gaagctctga ttgcattgtt
      781 aaatgaaggt gaggaactag aggagctgat gaagctttct cccgaggaat tactgctgcg
      841 atgggtgaac taccatctga ccaatgcagg atggcatacc atcagcaact tcagccaaga
      901 cattaaggac tcgagagcct attttcatct gcttaatcag attgccccta aaggtgggga
      961 agatggacct gccattgcca ttgacctttc aggaattaat gagacaaatg acctgaagcg
     1021 tgctggactc atgcttcaag aagcagataa actgggctgc aaacagtttg ttactcctgc
     1081 agatgtggtt tcaggcaatc ctaaacttaa tttagctttt gtagctaatt tgtttaacac
     1141 atacccgtgc ctgcacaagc cgaataataa tgacatcgat atgaatttac tggaaggaga
     1201 gagcaaggaa gagagaacat ttcggaactg gatgaattcc ttgggagtca acccatacat
     1261 taatcatttg tacagtgacc ttgcagatgc tttagtgatc tttcagctct atgagatgat
     1321 ccgagtgcca gtcaactgga gccatgtcaa caaacctcct tatcctgccc ttggagggaa
     1381 catgaagaag attgaaaact gtaactatgc agtggaactt gggaagaaca aggccaaatt
     1441 ctccttggtt ggcattgctg ggcaggacct aaatgaaggg aattcaacac ttaccctggc
     1501 attggtatgg cagctgatga gaaggtacac attgaatgtg ttatcggatc ttggagaggg
     1561 tgaaaaagta aatgatgaaa ttataattaa atgggtcaat cagactctta aaagtgcaaa
     1621 caaaaagact tctatttcca gcttcaagga taaatctata agcacaagtt tacctgtcct
     1681 agatttaata gatgccattg caccaaatgc agttcgtcaa gaaatgatca ggagagaaaa
     1741 cttatctgat gaggacaagc tgaacaatgc taaatacgcc atttcagttg ctcgaaagat
     1801 cggtgcccgg atatatgcat tacctgatga cctcgtagaa gtgaaaccaa agatggttat
     1861 gacggtgttt gcatgcttaa tgggaaaagg actgaacaga ataaaaagct ccggagggga
     1921 tccaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     1981 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     2041 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     2101 cgtgccctgg cccaccctcg tgaccacctt gacctacggc gtgcagtgct tcgcccgcta
     2161 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     2221 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     2281 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     2341 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaaggtct atatcaccgc
     2401 cgacaagcag aagaacggca tcaaggtgaa cttcaagacc cgccacaaca tcgaggacgg
     2461 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     2521 gctgcccgac aaccactacc tgagcaccca gtccaagctg agcaaagacc ccaacgagaa
     2581 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     2641 cgagctgtac aagtaagcgg ccgcgactct agatcataat caagcca
//
