LOCUS       sequence-84381-e        1416 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Peroxisomes, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1416)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-PMP-N-10
FEATURES             Location/Qualifiers
     source          1..1416
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      36..45
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      651..660
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(657..659,660..662,663..1376)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(702..723)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(963..982)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1310..1331
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctgccac catggcgccg gccgcgtcca
       61 ggctgcgggc cgaagccggg ctcggggcgc tgccgcggcg ggcgctcgcc cagtacttgc
      121 tcttcctgcg gctctacccg gtgctcacca aggcggccac cagtggcatt ttgtcagcac
      181 ttgggaactt cctggcccag atgattgaga agaagcggaa aaaagaaaac tctagaagtc
      241 tggatgtcgg tgggcctctg agatatgccg tttacgggtt cttcttcaca gggccgctga
      301 gtcacttctt ctacttcttc atggaacatt ggatccctcc tgaggtcccc ctggcagggc
      361 tcaggaggct tctcctggac cgcctcgtct ttgcaccggc cttcctcatg ttgttcttcc
      421 tcatcatgaa ctttctggag gggaaagacg cctcagcctt cgccgccaag atgagggggg
      481 gcttctggcc ggcgctgagg atgaactggc gggtgtggac gccactacag ttcatcaaca
      541 tcaactacgt ccctctgaag ttccgggtgc tcttcgccaa cctggcagct ctgttctggt
      601 atgcctacct ggcctccttg gggaagagcg gctccggtag cggaccggtc gccaccatgg
      661 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
      721 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
      781 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
      841 tgaccacctt gacctacggc gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc
      901 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
      961 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     1021 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     1081 tggagtacaa ctacaacagc cacaaggtct atatcaccgc cgacaagcag aagaacggca
     1141 tcaaggtgaa cttcaagacc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     1201 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     1261 tgagcaccca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     1321 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaagcgg
     1381 ccgcgactct agatcataat cagccatacc acattt
//
