LOCUS       sequence-84384-e        2993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Scaffold, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2993)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-PSD95-N-14
FEATURES             Location/Qualifiers
     source          1..2993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      31..40
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2245..2254
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2251..2253,2254..2256,2257..2970)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2296..2317)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2557..2576)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2904..2925
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccgctagcgc taccggactc agatctcgag gccaccatgg actgtctctg tatagtgaca
       61 accaagaaat accgctacca agatgaagac acgccccctc tggaacacag cccggcccac
      121 ctccccaacc aggccaattc tccccctgtg attgtcaaca cggacaccct agaagcccca
      181 ggatatgagt tgcaggtgaa tggaacagag ggggagatgg agtatgagga gatcacattg
      241 gaaaggggta actcaggtct gggcttcagc atcgcaggtg gcactgacaa cccgcacatc
      301 ggtgacgacc cgtccatttt tatcaccaag atcattcctg gtggggctgc agcccaggat
      361 ggccgcctca gggtcaatga cagcatcctg tttgtaaatg aagtggatgt tcgggaggtg
      421 acccattcag ctgcggtgga ggccctcaaa gaggcaggtt ccatcgttcg cctctatgtc
      481 atgcgccgga aacccccagc cgaaaaggtc atggagatca aactcatcaa agggcctaaa
      541 ggacttggct tcagcattgc ggggggcgtt gggaaccagc acatccctgg agataacagc
      601 atctatgtaa cgaagatcat cgaaggaggt gctgcccaca aggatggcag gttgcagatt
      661 ggagacaaga tcctggcggt caacagtgtg gggctggagg acgtcatgca cgaggatgcc
      721 gtggcagccc tgaagaacac atatgacgtt gtgtacctaa aggtggccaa gcccagcaat
      781 gcctacctga gtgacagcta tgctccccca gacatcacaa cctcgtattc tcagcacctg
      841 gacaatgaga tcagtcatag cagctacttg ggcactgact accccacagc catgaccccc
      901 acttcccctc ggcgctactc ccctgtggcc aaggacctgc tgggggagga agacattccc
      961 cgggaaccaa ggcggatcgt gatccatcgg ggctccaccg gcctgggctt caacatcgtg
     1021 ggcggcgagg atggtgaagg catcttcatc tccttcatcc ttgctggggg tccagccgac
     1081 ctcagtgggg agctacggaa gggggaccag atcctgtcgg tcaatggtgt tgacctccgc
     1141 aatgccagtc acgaacaggc tgccattgcc ctgaagaatg cgggtcagac ggtcacgatc
     1201 atcgctcagt ataaaccaga agagtatagt cgattcgagg ccaagatcca tgatcttcgg
     1261 gaacagctca tgaatagtag cctaggctca gggactgcat ccttgcgaag caaccccaag
     1321 aggggcttct acattagggc cctgtttgat tacgacaaga ccaaggactg cggtttcttg
     1381 agccaggccc tgagcttccg cttcggggat gtgcttcatg tcattgacgc tggtgacgaa
     1441 gagtggtggc aagcacggcg ggtccactcc gacagtgaga ccgacgacat tggcttcatt
     1501 cccagcaaac ggcgggtcga gcgacgagag tggtcaaggt taaaggccaa ggactggggc
     1561 tccagctctg gatcacaggg tcgagaagac tcggttctga gctatgagac ggtgacccag
     1621 atggaagtgc actatgctcg tcccatcatc atccttggac ccaccaaaga ccgtgccaac
     1681 gatgatcttc tctccgagtt ccccgacaag tttggatcct gtgtccctca tacgacacgt
     1741 cctaagcggg aatatgagat agacggccgg gattaccact ttgtctcctc ccgggagaaa
     1801 atggagaagg acatccaggc acacaagttc attgaggctg gccagtacaa cagccacctc
     1861 tatgggacca gcgtccagtc tgtgcgagag gtagcagagc aggggaagca ctgcatcctc
     1921 gatgtctcgg ccaatgccgt gcggcggctg caggcggccc acctgcaccc catcgccatc
     1981 ttcatccgtc cccgctccct ggagaatgtg ctagagatca ataagcggat cacagaggag
     2041 caagcccgga aagccttcga cagagccacg aagctggagc aggagttcac agagtgcttc
     2101 tcagccatcg tagagggcga cagctttgaa gagatctatc acaaagtgaa acgtgtcatt
     2161 gaagacctct caggccccta catctgggtc ccagcccgag agagactcgg cagcgcatcc
     2221 ggcggaagcg gaagcggacc ggtcgccacc atggtgagca agggcgagga gctgttcacc
     2281 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     2341 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     2401 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttgaccta cggcgtgcag
     2461 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     2521 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     2581 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     2641 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaag
     2701 gtctatatca ccgccgacaa gcagaagaac ggcatcaagg tgaacttcaa gacccgccac
     2761 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     2821 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccaa gctgagcaaa
     2881 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     2941 actctcggca tggacgagct gtacaagtaa gcggccgcga ctctagatca taa
//
