LOCUS       sequence-84385-e        3994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesion Kinase, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3994)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-PTK2-C-10
FEATURES             Location/Qualifiers
     source          1..3994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(35..37,38..40,41..751)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(80..101)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(341..360)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     688..709
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acggtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt
       61 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      121 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      181 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttga cctacggcgt
      241 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      301 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      361 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      421 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
      481 caaggtctat atcaccgccg acaagcagaa gaacggcatc aaggtgaact tcaagacccg
      541 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      601 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag
      661 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
      721 gatcactctc ggcatggacg agctgtacaa gtccggactc agatctagcg gtggcagtgg
      781 agcagctgct taccttgacc ccaacttgaa tcacacacca aattcgagta ctaagactca
      841 cctgggtact ggtatggaac gttctcctgg tgcaatggag cgagtattaa aggtctttca
      901 ttattttgaa agcaatagtg agccaaccac ctgggccagt attatcaggc atggagatgc
      961 tactgatgtc aggggcatca ttcagaagat agtggacagt cacaaagtaa agcatgtggc
     1021 ctgctatgga ttccgcctca gtcacctgcg gtcagaggag gttcactggc ttcacgtgga
     1081 tatgggcgtc tccagtgtga gggagaagta tgagcttgct cacccaccag aggagtggaa
     1141 atatgaattg agaattcgtt atttgccaaa aggatttcta aaccagttta ctgaagataa
     1201 gccaactttg aatttcttct atcaacaggt gaagagcgat tatatgttag agatagctga
     1261 tcaagtggac caggaaattg ctttgaagtt gggttgtcta gaaatacggc gatcatactg
     1321 ggagatgcgg ggcaatgcac tagaaaagaa gtctaactat gaagtattag aaaaagatgt
     1381 tggtttaaag cgattttttc ctaagagttt actggattct gtcaaggcca aaacactaag
     1441 aaaactgatc caacaaacat ttagacaatt tgccaacctt aatagagaag aaagtattct
     1501 gaaattcttt gagatcctgt ctccagtcta cagatttgat aaggaatgct tcaagtgtgc
     1561 tcttggttca agctggatta tttcagtgga actggcaatc ggcccagaag aaggaatcag
     1621 ttacctaacg gacaagggct gcaatcccac acatcttgct gacttcactc aagtgcaaac
     1681 cattcagtat tcaaacagtg aagacaagga cagaaaagga atgctacaac taaaaatagc
     1741 aggtgcaccc gagcctctga cagtgacggc accatcccta accattgcgg agaatatggc
     1801 tgacctaata gatgggtact gccggctggt gaatggaacc tcgcagtcat ttatcatcag
     1861 acctcagaaa gaaggtgaac gggctttgcc atcaatacca aagttggcca acagcgaaaa
     1921 gcaaggcatg cggacacacg ccgtctctgt gtcagaaaca gatgattatg ctgagattat
     1981 agatgaagaa gatacttaca ccatgccctc aaccagggat tatgagattc aaagagaaag
     2041 aatagaactt ggacgatgta ttggagaagg ccaatttgga gatgtacatc aaggcattta
     2101 tatgagtcca gagaatccag ctttggcggt tgcaattaaa acatgtaaaa actgtacttc
     2161 ggacagcgtg agagagaaat ttcttcaaga agccttaaca atgcgtcagt ttgaccatcc
     2221 tcatattgtg aagctgattg gagtcatcac agagaatcct gtctggataa tcatggagct
     2281 gtgcacactt ggagagctga ggtcattttt gcaagtaagg aaatacagtt tggatctagc
     2341 atctttgatc ctgtatgcct atcagcttag tacagctctt gcatatctag agagcaaaag
     2401 atttgtacac agggacattg ctgctcggaa tgttctggtg tcctcaaatg attgtgtaaa
     2461 attaggagac tttggattat cccgatatat ggaagatagt acttactaca aagcttccaa
     2521 aggaaaattg cctattaaat ggatggctcc agagtcaatc aattttcgac gttttacctc
     2581 agctagtgac gtatggatgt ttggtgtgtg tatgtgggag atactgatgc atggtgtgaa
     2641 gccttttcaa ggagtgaaga acaatgatgt aatcggtcga attgaaaatg gggaaagatt
     2701 accaatgcct ccaaattgtc ctcctaccct ctacagcctt atgacgaaat gctgggccta
     2761 tgaccccagc aggcggccca ggtttactga acttaaagct cagctcagca caatcctgga
     2821 ggaagagaag gctcagcaag aagagcgcat gaggatggag tccagaagac aggccacagt
     2881 gtcctgggac tccggagggt ctgatgaagc accgcccaag cccagcagac cgggttatcc
     2941 cagtccgagg tccagcgaag gattttatcc cagcccacag cacatggtac aaaccaatca
     3001 ttaccaggtt tctggctacc ctggttcaca tggaatcaca gccatggctg gcagcatcta
     3061 tccaggtcag gcatctcttt tggaccaaac agattcatgg aatcatagac ctcaggagat
     3121 agcaatgtgg cagcccaatg tggaggactc tacagtattg gacctgcgag ggattgggca
     3181 agtgttgcca acccatctga tggaagagcg tctaatccga cagcaacagg aaatggaaga
     3241 agatcagcgc tggctggaaa aagaggaaag atttctgaaa cctgatgtga gactctctcg
     3301 aggcagtatt gacagggagg atggaagtct tcagggtccg attggaaacc aacatatata
     3361 tcagcctgtg ggtaaaccag atcctgcagc tccaccaaag aaaccgcctc gccctggagc
     3421 tcccggtcat ctgggaagcc ttgccagcct cagcagccct gctgacagct acaacgaggg
     3481 tgtcaagcca tggaggcttc agccccagga aatcagcccc cctcctactg ccaacctgga
     3541 ccggtcgaat gataaggtgt acgagaatgt gacgggcctg gtgaaagctg tcatcgagat
     3601 gtccagtaaa atccagccag ccccaccaga ggagtatgtc cctatggtga aggaagtcgg
     3661 cttggccctg aggacattat tggccactgt ggatgagacc attcccctcc taccagccag
     3721 cacccaccga gagattgaga tggcacagaa gctattgaac tctgacctgg gtgagctcat
     3781 caacaagatg aaactggccc agcagtatgt catgaccagc ctccagcaag agtacaaaaa
     3841 gcaaatgctg actgctgctc acgccctggc tgtggatgcc aaaaacttac tcgatgtcat
     3901 tgaccaagca agactgaaaa tgcttgggca gacgagacca cactgaggat ccaccggatc
     3961 tagataactg atcataatca gccataccac attt
//
