LOCUS       sequence-84387-e        1467 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Ras GTPase, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1467)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Rab4a-7
FEATURES             Location/Qualifiers
     source          1..1467
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(26..28,29..31,32..742)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(71..92)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(332..351)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     679..700
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tccgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt
       61 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
      121 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
      181 caagctgccc gtgccctggc ccaccctcgt gaccaccttg acctacggcg tgcagtgctt
      241 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
      301 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
      361 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
      421 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaaggtcta
      481 tatcaccgcc gacaagcaga agaacggcat caaggtgaac ttcaagaccc gccacaacat
      541 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
      601 ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga gcaaagaccc
      661 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
      721 cggcatggac gagctgtaca agtccggact cagatctcga gccatgtcgc agacggccat
      781 gtccgaaacc tacgattttt tgtttaagtt cttggttatt ggaaatgcag gaactggcaa
      841 atcttgctta cttcatcagt ttattgaaaa aaaattcaaa gatgactcaa atcatacaat
      901 aggagtggaa tttggttcaa agataataaa tgttggtggt aaatatgtaa agttacaaat
      961 atgggataca gcaggacaag aacgattcag gtccgtgacg agaagttatt accgaggcgc
     1021 ggccggggct ctcctcgtct atgatatcac cagccgagaa acctacaatg cgcttactaa
     1081 ttggttaaca gatgcccgaa tgctggcgag ccagaacatt gtgatcatcc tttgtggaaa
     1141 caagaaggac ctggatgcag atcgtgaagt taccttctta gaagcctcca gatttgctca
     1201 agaaaatgag ctgatgtttt tggaaacaag tgcgctcaca ggggagaatg tagaagaggc
     1261 ttttgtacag tgtgcaagaa aaatacttaa caaaatcgaa tcaggtgagc tggacccaga
     1321 aagaatgggc tcaggtattc agtacggaga tgctgccttg agacagctga ggtcaccgcg
     1381 gcgcgcacag gccccgaacg ctcaggagtg tggttgttag ggatccaccg gatctagata
     1441 actgatcata atcagccata ccacatt
//
