LOCUS       sequence-84389-e        1444 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Ras GTPase, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1444)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Rab7a-7
FEATURES             Location/Qualifiers
     source          1..1444
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(36..38,39..41,42..752)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(81..102)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(342..361)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     689..710
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgacgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggagctgt
       61 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
      121 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
      181 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttg acctacggcg
      241 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
      301 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
      361 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
      421 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
      481 acaaggtcta tatcaccgcc gacaagcaga agaacggcat caaggtgaac ttcaagaccc
      541 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
      601 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga
      661 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
      721 ggatcactct cggcatggac gagctgtaca agtccggact cagatctcga gccatgacct
      781 ctaggaagaa agtgttgctg aaggttatca tcctgggaga ttctggagtc gggaagacat
      841 cactcatgaa ccagtatgtg aataagaaat tcagcaatca gtacaaagcc acaataggag
      901 ctgactttct gaccaaggag gtgatggtgg atgacaggct agtcacaatg cagatatggg
      961 acacagcagg acaggaacgg ttccagtctc tcggtgtggc cttctacaga ggtgcagact
     1021 gctgcgttct ggtatttgat gtgactgccc ccaacacatt caaaacccta gatagctgga
     1081 gagatgagtt tctcatccag gccagtcccc gagatcctga aaacttccca tttgttgtgt
     1141 tgggaaacaa gattgacctc gaaaacagac aagtggccac aaagcgggca caggcctggt
     1201 gctacagcaa aaacaacatt ccctactttg agaccagtgc caaggaggcc atcaacgtgg
     1261 agcaggcgtt ccagacgatt gcacggaatg cacttaagca ggaaacggag gtggagctgt
     1321 acaacgaatt tcctgaacct atcaaactgg acaagaatga ccgggccaag gcctcggcag
     1381 aaagctgcag ttgctgagga tccaccggat ctagataact gatcataatc agccatacca
     1441 catt
//
