LOCUS       sequence-84392-e        2750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Centromere, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2750)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Rad21-N-18
FEATURES             Location/Qualifiers
     source          1..2750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1986..1995
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1992..1994,1995..1997,1998..2711)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2037..2058)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2298..2317)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2645..2666
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggactcag atctcgaggc caccatgttc tacgcacatt
       61 ttgttctcag taaaagaggg cctctggcca aaatttggct agcggcccat tgggataaga
      121 agctaaccaa agcccatgtg ttcgagtgta atttagagag cagcgtggag agtatcatct
      181 caccaaaggt gaaaatggca ttacggacat caggacatct cttactggga gtagttcgaa
      241 tctatcacag gaaagccaaa taccttcttg cagactgtaa tgaagcattc attaagataa
      301 agatggcttt tcggccaggt gtggttgacc tgcctgagga aaatcgggaa gcagcttata
      361 atgccattac tttacctgaa gaatttcatg actttgatca gccactgcct gacttagatg
      421 acatcgatgt ggcccagcag ttcagcttga atcagagtag agtggaagag ataaccatga
      481 gagaagaagt tgggaacatc agtattttac aagaaaatga ttttggtgat tttggaatgg
      541 atgatcgtga gataatgaga gaaggcagtg cttttgagga tgacgacatg ttagtaagca
      601 ctactacttc taacctccta ttagagtctg aacagagcac cagcaatctg aatgagaaaa
      661 ttaaccattt agaatatgaa gatcaatata aggatgataa ttttggagaa ggaaatgatg
      721 gtggaatatt agatgacaaa cttattagta ataatgatgg cggtatcttt gatgatcccc
      781 ctgccctctc tgaggcaggg gtgatgttgc cagagcagcc tgcacatgac gatatggatg
      841 aggatgataa tgtatcaatg ggtgggcctg atagtcctga ttcagtggat cccgttgaac
      901 caatgccaac catgactgat caaacaacac ttgttccaaa tgaggaagaa gcatttgcat
      961 tggagcctat tgatataact gttaaagaaa caaaagccaa gaggaagagg aagctaattg
     1021 ttgacagtgt caaagagttg gatagcaaga caattagagc ccaacttagt gattattcag
     1081 atattgttac tactttggat ctggcaccgc ccaccaagaa attgatgatg tggaaagaga
     1141 caggaggagt agaaaaactg ttttctttac ctgctcagcc tttgtggaat aacagactac
     1201 tgaagctctt tacacgctgt cttacaccgc ttgtaccaga agaccttaga aaaaggagga
     1261 aaggaggaga ggcagataat ttggatgaat tcctcaaaga atttgaaaat ccagaggttc
     1321 ctagagagga ccagcaacag cagcatcagc agcgtgatgt tatcgatgag cccattattg
     1381 aagagccaag ccgcctccag gagtcagtga tggaggccag cagaacaaac atagatgagt
     1441 cagctatgcc tccaccacca cctcagggag ttaagcgaaa agctggacaa attgacccag
     1501 agcctgtgat gcctcctcag caggtagagc agatggaaat accacctgta gagcttcccc
     1561 cagaagaacc tccaaatatc tgtcagctaa taccagagtt agaacttctg ccagaaaaag
     1621 agaaggagaa agagaaggaa aaagaagatg atgaagagga agaggatgaa gatgcatcag
     1681 ggggcgatca agatcaggaa gaaagaagat ggaacaaaag gactcagcag atgcttcatg
     1741 gtcttcagcg tgctcttgct aaaactggag ctgaatctat cagtttgctt gagttatgtc
     1801 gaaatacgaa cagaaaacaa gctgccgcaa agttctacag cttcttggtt cttaaaaagc
     1861 agcaagctat tgagctgaca caggaagaac cgtacagtga catcatcgca acacctggac
     1921 caaggttcca tattataagt cgcggaggca gtggtggggt accgcgggcc cgggatccac
     1981 cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     2041 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     2101 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     2161 cctggcccac cctcgtgacc accttgacct acggcgtgca gtgcttcgcc cgctaccccg
     2221 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     2281 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     2341 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     2401 tcctggggca caagctggag tacaactaca acagccacaa ggtctatatc accgccgaca
     2461 agcagaagaa cggcatcaag gtgaacttca agacccgcca caacatcgag gacggcagcg
     2521 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     2581 ccgacaacca ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg
     2641 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     2701 tgtacaagta agcggccgcg actctagatc ataatcagcc ataccacatt
//
