LOCUS       sequence-84394-e        1149 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1149)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Sec61-N-18
FEATURES             Location/Qualifiers
     source          1..1149
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..323
                     /codon_start=1
                     /gene="SEC61B"
                     /product="human Sec61-beta subunit of the translocon in the
                     endoplasmic reticulum"
                     /label=Sec61-beta
                     /translation="MPGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAG
                     RTTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS"
     regulatory      372..381
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(378..380,381..383,384..1097)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(423..444)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(684..703)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1031..1052
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccattgctag tgacgtcaga tccgctagcg ccaccatgcc tggtccgacc cccagtggca
       61 ctaacgtggg atcctcaggg cgctctccca gcaaagcagt ggccgcccgg gcggcgggat
      121 ccactgtccg gcagaggaaa aatgccagct gtgggacaag gagtgcaggc cgcacaacct
      181 cggcaggcac cggggggatg tggcgattct acacagaaga ttcacctggg ctcaaagttg
      241 gccctgttcc agtattggtt atgagtcttc tgttcatcgc ttctgtattt atgttgcaca
      301 tttggggcaa gtacactcgt tcgggacgaa ttctgcagtc gacggtaccg cgggcccggg
      361 atccaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
      421 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
      481 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
      541 ccgtgccctg gcccaccctc gtgaccacct tgacctacgg cgtgcagtgc ttcgcccgct
      601 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
      661 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
      721 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
      781 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaaggtc tatatcaccg
      841 ccgacaagca gaagaacggc atcaaggtga acttcaagac ccgccacaac atcgaggacg
      901 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
      961 tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac cccaacgaga
     1021 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     1081 acgagctgta caagtaagcg gccgcgactc tagatcataa tcagccatac cacattgtag
     1141 aagtttccc
//
