LOCUS       sequence-84395-e        1934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Scaffold, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1934)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Sequestosome1-C-18
FEATURES             Location/Qualifiers
     source          1..1934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      31..40
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(37..39,40..42,43..753)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(82..103)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(343..362)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     690..711
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtgacgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggagctg
       61 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
      121 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
      181 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt gacctacggc
      241 gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
      301 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
      361 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
      421 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
      481 cacaaggtct atatcaccgc cgacaagcag aagaacggca tcaaggtgaa cttcaagacc
      541 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
      601 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg
      661 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
      721 gggatcactc tcggcatgga cgagctgtac aagtccggac tcagatctcg agagagcgga
      781 ggtagcgcat ccggcggaag cggaggagcc atgtcctacg tgaaggatga catcttccga
      841 atctacatta aagagaaaaa agagtgccgg cgggaccacc gcccaccgtg tgctcaggag
      901 gcgccccgca acatggtgca ccccaatgtg atctgcgatg gctgcaatgg gcctgtggta
      961 ggaacccgct acaagtgcag cgtctgccca gactacgact tgtgtagcgt ctgcgaggga
     1021 aagggcttgc accgggggca caccaagctc gcattcccca gccccttcgg gcacctgtct
     1081 gagggcttct cgcacagccg ctggctccgg aaggtgaaac acggacactt cgggtggcca
     1141 ggatgggaaa tgggtccacc aggaaactgg agcccacgtc ctcctcgtgc aggggaggcc
     1201 cgccctggcc ccacggcaga atcagcttct ggtccatcgg aggatccgag tgtgaatttc
     1261 ctgaagaacg ttggggagag tgtggcagct gcccttagcc ctctgggcat tgaagttgat
     1321 atcgatgtgg agcacggagg gaaaagaagc cgcctgaccc ccgtctctcc agagagttcc
     1381 agcacagagg agaagagcag ctcacagcca agcagctgct gctctgatcc cagcaagccg
     1441 ggtgggaatg ttgagggcgc cacgcagtct ctggcggagc agatgagaaa gatcgccttg
     1501 gagtccgagg ggcgccctga ggaacagatg gagtcggata actgttcagg aggagatgat
     1561 gactggaccc atctgtcttc aaaagaagtg gacccgtcta caggtgaact ccagtcccta
     1621 cagatgccag aatccgaagg gccaagctct ctggacccct cccaggaggg acccacaggg
     1681 ctgaaggaag ctgccttgta cccacatctc ccgccagagg ctgacccgcg gctgattgag
     1741 tccctctccc agatgctgtc catgggcttc tctgatgaag gcggctggct caccaggctc
     1801 ctgcagacca agaactatga catcggagcg gctctggaca ccatccagta ttcaaagcat
     1861 cccccgccgt tgtgagaatt ctgcagtcga cggtaccgcg ggcccgggat ccaccggatc
     1921 tagataactg atca
//
