LOCUS       sequence-84396-e        1932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Scaffold, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1932)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Sequestosome1-N-18
FEATURES             Location/Qualifiers
     source          1..1932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      32..41
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1154..1163
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1160..1162,1163..1165,1166..1879)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1205..1226)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1466..1485)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1813..1834
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acccttgcta ggtgacgtca gatccgctag cgccaccatg gccatgtcct acgtgaagga
       61 tgacatcttc cgaatctaca ttaaagagaa aaaagagtgc cggcgggacc accgcccacc
      121 gtgtgctcag gaggcgcccc gcaacatggt gcaccccaat gtgatctgcg atggctgcaa
      181 tgggcctgtg gtaggaaccc gctacaagtg cagcgtctgc ccagactacg acttgtgtag
      241 cgtctgcgag ggaaagggct tgcaccgggg gcacaccaag ctcgcattcc ccagcccctt
      301 cgggcacctg tctgagggct tctcgcacag ccgctggctc cggaaggtga aacacggaca
      361 cttcgggtgg ccaggatggg aaatgggtcc accaggaaac tggagcccac gtcctcctcg
      421 tgcaggggag gcccgccctg gccccacggc agaatcagct tctggtccat cggaggatcc
      481 gagtgtgaat ttcctgaaga acgttgggga gagtgtggca gctgccctta gccctctggg
      541 cattgaagtt gatatcgatg tggagcacgg agggaaaaga agccgcctga cccccgtctc
      601 tccagagagt tccagcacag aggagaagag cagctcacag ccaagcagct gctgctctga
      661 tcccagcaag ccgggtggga atgttgaggg cgccacgcag tctctggcgg agcagatgag
      721 aaagatcgcc ttggagtccg aggggcgccc tgaggaacag atggagtcgg ataactgttc
      781 aggaggagat gatgactgga cccatctgtc ttcaaaagaa gtggacccgt ctacaggtga
      841 actccagtcc ctacagatgc cagaatccga agggccaagc tctctggacc cctcccagga
      901 gggacccaca gggctgaagg aagctgcctt gtacccacat ctcccgccag aggctgaccc
      961 gcggctgatt gagtccctct cccagatgct gtccatgggc ttctctgatg aaggcggctg
     1021 gctcaccagg ctcctgcaga ccaagaacta tgacatcgga gcggctctgg acaccatcca
     1081 gtattcaaag catcccccgc cgttgagcag cggtggagca agcgcagcca gtggtagcgc
     1141 agcgggaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     1201 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     1261 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     1321 gcccgtgccc tggcccaccc tcgtgaccac cttgacctac ggcgtgcagt gcttcgcccg
     1381 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     1441 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     1501 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     1561 cggcaacatc ctggggcaca agctggagta caactacaac agccacaagg tctatatcac
     1621 cgccgacaag cagaagaacg gcatcaaggt gaacttcaag acccgccaca acatcgagga
     1681 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     1741 gctgctgccc gacaaccact acctgagcac ccagtccaag ctgagcaaag accccaacga
     1801 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     1861 ggacgagctg tacaagtaag cggccgcgac tctagatcat aatcagccat accacattgt
     1921 agaagaattg gc
//
