LOCUS       sequence-84397-e        1593 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin Bundling, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1593)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-sEspin-C-18
FEATURES             Location/Qualifiers
     source          1..1593
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(29..31,32..34,35..745)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(74..95)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(335..354)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     682..703
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agatccgcta gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg
       61 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
      121 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
      181 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttgacctacg gcgtgcagtg
      241 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
      301 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
      361 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
      421 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaaggt
      481 ctatatcacc gccgacaagc agaagaacgg catcaaggtg aacttcaaga cccgccacaa
      541 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
      601 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccaagc tgagcaaaga
      661 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
      721 tctcggcatg gacgagctgt acaagtccgg actcagatct agtagcggag gtagcgcatc
      781 cggcggaagc ggaggaagca actcccaggg gcctctacgt gggggccgaa tgcccagcac
      841 caaatctttc aacatgatgt ccccaacggg tgacaactcg gagttgctgg ctgaaataaa
      901 agcaggcaag agtctgaaac caacaccgca gagcaagggg ctgacaacag tgttctcagg
      961 cagcgggcag ccagcctccc aggtaggcac tggacgggtg ccccgcccgg gctcccagtg
     1021 cctgcccagt gctcagccct accgcttctc ccggcagcct gagtcaccac agcctgcggt
     1081 gtcacctggg ccatctcggg cccggagccc caccccacca gcctctgggc ctcagccact
     1141 gctcaatggc agcatagtac cggcaccacc tgccacccta gcaccaggag tgcatctgga
     1201 tgtggaggcc ctcatcccca cacttgatga gcagggccgg cccatcccgg agtggaagcg
     1261 ccaggtgatg gtccggaagc tgcagcagaa aatgcaggag gaagaggagc agcggaggaa
     1321 ggaagaagag gaggaggccc ggctggccag cctgcccgcc tggagacgag acattcttag
     1381 gaagaagctg gaggaggaga gggagcagaa gcgaaaagag gaggagcggc agaagctgga
     1441 ggaaatccag agggcgaaag aacagtcgga gaagctgcgg acactaggat acgacgaggc
     1501 caagctcgcg ccctggcagc gacaggtcat cttgaagaag ggggagatcc ctaagtaagg
     1561 atccaccgga tctagataac tgatcataat cag
//
