LOCUS       sequence-84398-e        1585 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin Bundling, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1585)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-sEspin-N-18
FEATURES             Location/Qualifiers
     source          1..1585
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      835..844
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(841..843,844..846,847..1560)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(886..907)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1147..1166)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1494..1515
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agtgacgtca gatccgctag cgccaccatg aactcccagg ggcctctacg tgggggccga
       61 atgcccagca ccaaatcttt caacatgatg tccccaacgg gtgacaactc ggagttgctg
      121 gctgaaataa aagcaggcaa gagtctgaaa ccaacaccgc agagcaaggg gctgacaaca
      181 gtgttctcag gcagcgggca gccagcctcc caggtaggca ctggacgggt gccccgcccg
      241 ggctcccagt gcctgcccag tgctcagccc taccgcttct cccggcagcc tgagtcacca
      301 cagcctgcgg tgtcacctgg gccatctcgg gcccggagcc ccaccccacc agcctctggg
      361 cctcagccac tgctcaatgg cagcatagta ccggcaccac ctgccaccct agcaccagga
      421 gtgcatctgg atgtggaggc cctcatcccc acacttgatg agcagggccg gcccatcccg
      481 gagtggaagc gccaggtgat ggtccggaag ctgcagcaga aaatgcagga ggaagaggag
      541 cagcggagga aggaagaaga ggaggaggcc cggctggcca gcctgcccgc ctggagacga
      601 gacattctta ggaagaagct ggaggaggag agggagcaga agcgaaaaga ggaggagcgg
      661 cagaagctgg aggaaatcca gagggcgaaa gaacagtcgg agaagctgcg gacactagga
      721 tacgacgagg ccaagctcgc gccctggcag cgacaggtca tcttgaagaa gggggagatc
      781 cctaagagca gcggtggagc aagcgcagcc agtggtagcg cggatccacc ggtcgccacc
      841 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
      901 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      961 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     1021 ctcgtgacca ccttgaccta cggcgtgcag tgcttcgccc gctaccccga ccacatgaag
     1081 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     1141 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     1201 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     1261 aagctggagt acaactacaa cagccacaag gtctatatca ccgccgacaa gcagaagaac
     1321 ggcatcaagg tgaacttcaa gacccgccac aacatcgagg acggcagcgt gcagctcgcc
     1381 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     1441 tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     1501 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
     1561 gcggccgcga ctctagatca taatc
//
