LOCUS       sequence-84401-e        2130 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cilium, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2130)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-SSTR3-N-17
FEATURES             Location/Qualifiers
     source          1..2130
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1371..1380
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1377..1379,1380..1382,1383..2096)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1422..1443)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1683..1702)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2030..2051
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cagatccgct agcgctaccg gactcagatc tcgagaccgc catggccact gttacctatc
       61 cttcatctga gcctacgacc ttggaccctg ggaacgcatc ctcgacctgg cccctggata
      121 ccaccctggg gaacacatcc gctggcgcta gcctgacagg cctggctgtc agtggcatct
      181 tgatctctct ggtgtacctg gtggtgtgcg tggtgggttt gctgggcaac tcgctggtga
      241 tctacgtggt cctgcggcac acgtccagcc catcagtgac cagtgtctat atcctcaacc
      301 tggctctggc tgatgagctc ttcatgctag ggctaccctt cctggctgct cagaacgccc
      361 tgtcctactg gcccttcgga tctctcatgt gccgtctggt catggccgtg gatggcatca
      421 accagttcac cagcatcttc tgcctcaccg tcatgagtgt ggaccgctat ctggctgtgg
      481 tgcaccccac acgctcagcc cgctggcgca cggcaccagt ggctcgcacg gtcagtgcag
      541 ctgtctgggt ggcctcggct gtggtggtgc tgcctgtggt tgtgttctca ggagtgcccc
      601 ggggcatgag cacgtgccac atgcagtggc cagagccagc ggctgcctgg cgaacagcct
      661 tcatcatcta cacggccgca ctgggcttct ttgggcccct gctggtcatc tgcttgtgct
      721 acttgctcat tgtggtaaag gtgcggtcga ccacccggcg ggtgcgggcg ccctcgtgtc
      781 agtgggtaca ggcacccgca tgccagcggc gacgccgctc tgagcgcagg gtcacacgca
      841 tggtggtggc cgtggtggca ctcttcgtcc tctgctggat gcctttctat ctgctcaaca
      901 tcgtcaatgt ggtgtgcccg ctgccggagg agcccgcctt cttcggcctc tacttcctgg
      961 tggtggcgct gccctacgcc aacagctgcg caaaccccat cctctacggc ttcctctcct
     1021 accgcttcaa gcagggcttt cgcaggatcc tgctaagacc atcacgtcgc attcggagcc
     1081 aggagccagg gtcgggacct ccagagaaga ctgaagagga ggaggatgaa gaagaagaag
     1141 agagaaggga agaggaggag cggaggatgc agagagggca ggagatgaac gggaggctca
     1201 gtcagatcgc acaggctggc actagtggac aacagccacg gccctgcaca gggactgcta
     1261 aggagcagca gcttctgccc caggaggcca cagctgggga caaggccagc acactgagcc
     1321 atctgggaat tctgcagtcg acggtaccgc gggcccggga tccaccggtc gccaccatgg
     1381 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     1441 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     1501 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     1561 tgaccacctt gacctacggc gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc
     1621 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     1681 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     1741 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     1801 tggagtacaa ctacaacagc cacaaggtct atatcaccgc cgacaagcag aagaacggca
     1861 tcaaggtgaa cttcaagacc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     1921 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     1981 tgagcaccca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     2041 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaagcgg
     2101 ccgcgactct agatcataat cagccatacc
//
