LOCUS       sequence-84402-e        1709 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1709)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Stomatin-C-14
FEATURES             Location/Qualifiers
     source          1..1709
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      32..41
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(38..40,41..43,44..754)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(83..104)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(344..363)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     691..712
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gaccgttcag atcccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct
       61 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
      121 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
      181 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tgacctacgg
      241 cgtgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
      301 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
      361 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
      421 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
      481 ccacaaggtc tatatcaccg ccgacaagca gaagaacggc atcaaggtga acttcaagac
      541 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
      601 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct
      661 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
      721 cgggatcact ctcggcatgg acgagctgta caagtccgga agtgctggga gcgcaagcgg
      781 aagcggaggc tccggtgccg agaagcggca cacacgggac tccgaagccc agcggctccc
      841 cgactccttc aaggacagcc ccagtaaggg ccttggacct tgcggatgga ttttggtggc
      901 gttctcattc ttattcaccg ttataacttt cccaatctca atatggatgt gcataaagat
      961 tataaaagag tatgaaagag ccatcatctt tagattgggt cgcattttac aaggaggagc
     1021 caaaggacct ggtttgtttt ttattctgcc atgcactgac agcttcatca aagtggacat
     1081 gagaactatt tcatttgata ttcctcctca ggagatcctc acaaaggatt cagtgacaat
     1141 tagcgtggat ggtgtggtct attaccgcgt tcagaatgca accctggctg tggcaaatat
     1201 caccaacgct gactcagcaa cccgtctttt ggcacaaact actctgagga atgttctggg
     1261 caccaagaat ctttctcaga tcctctctga cagagaagaa attgcacaca acatgcagtc
     1321 tactctggat gatgccactg atgcctgggg aataaaggtg gagcgtgtgg aaattaagga
     1381 tgtgaaacta cctgtgcagc tccagagagc tatggctgca gaagcagaag cgtcccgcga
     1441 ggcccgcgcc aaggttattg cagccgaagg agaaatgaat gcatccaggg ctctgaaaga
     1501 agcctccatg gtcatcactg aatctcctgc agcccttcag ctccgatacc tgcagacact
     1561 gaccaccatt gctgctgaga aaaactcaac aattgtcttc cctctgccca tagatatgct
     1621 gcaaggaatc ataggggcaa aacacagcca tctaggctag ggatccaccg gatctagata
     1681 actgatcata atcagccata ccacattgt
//
