LOCUS       sequence-84404-e        6376 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane/Actin, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6376)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 6376)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Supervillin-45
FEATURES             Location/Qualifiers
     source          1..6376
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(28..30,31..33,34..744)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(73..94)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(334..353)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     681..702
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
       61 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      121 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      181 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tgacctacgg cgtgcagtgc
      241 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      301 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      361 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      421 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaaggtc
      481 tatatcaccg ccgacaagca gaagaacggc atcaaggtga acttcaagac ccgccacaac
      541 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      601 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac
      661 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
      721 ctcggcatgg acgagctgta caagtccgga ctcagatctc gagctcaagc ttcgaattct
      781 gcagtcgacg gtaccttcga tgctcagctt ttagctgccc ttcctaaagt tgcagaatta
      841 agaaaaatct ttgaaccaaa gaggaaagaa tttttagaaa tgaaaagaaa agaaagaatt
      901 gcccggcgct tagaaggaat tgaaaccgac acgcagccca tcctcttgca gagctgcacg
      961 ggcttggtga ctcatcgcct gctggaggag gacacaccca gatacatgcg tgccacagac
     1021 ccggccagcc cgcacattgg tcgatcaaat gaagaagaag aaacttcaga ttcttcacta
     1081 gaaaagcaga ctcgatccaa acagtgcaca gaaacctcag gcatccatgc tgactcgccc
     1141 tacagttcag gcatcatgga cacccagagc ctcgagtcca aagccgaaag aatcgccagg
     1201 tacaaagcag agagaagacg gcagctggca gaaaagtatg ggctcaccct ggatccggaa
     1261 gcagactctg aaactccatc tcgatacagc aggtccagaa aggaccccga ggctgcggag
     1321 aaaaggggag taagaagcga gcgatcggcc gagtccagca gagacgccgg ctcatcctac
     1381 tccaggactg agctctcggg gctcaggacc tgcgtggctg agtccaagga ctanggcctg
     1441 caccggagtg acggtgtttc cgacacagag gtgctgctca atgcagaaaa ccaaagacgc
     1501 ggtcaagagc ccagtgccac cgggctggcc cgagacctgc cccttgcagg ggaggtctcc
     1561 tcttctttct cattctccgg gcgagactct gccctcggtg aagtgccaag gtccccaaag
     1621 gcagtgcaca gcctgcccag tccatcgcct gggcagccgg cctccccaag ccactccacc
     1681 agtgacctgc cactccctgc ggaggctcga gccaggtcaa cttcaaattc agaaatgcca
     1741 gctgccgagg atgaagaaaa ggtcgatgag cgagcgaggc tgagcgtcgc tgctaagagg
     1801 ctgctcttca gggagatgga gaagtcgttt gacgagaaga gcgtcccaaa gcgccgctcc
     1861 cgcaatgcgg ccgtggagca gaggctgcgg cgcctccagg accggtccca cacgcagccc
     1921 gtcaccaccg aggaggtggt cattgcagcc acattgcagg cgtctgctca ccaaaaggca
     1981 ctagccagag accagacaaa cgagagcaaa gattctgctg agcagggaga acctgactcc
     2041 tccactctga gcttagcgga gaagctggcc ttgtttaaca aactatctca gccggtctca
     2101 aaagccattt caacacggaa cagattagac atgagacaga ggaggatgaa tgctcgttat
     2161 caaacccagc cggtcacgct tggagaagtg gaacaggtgc agagtggcaa gctcatggct
     2221 ttctctccca ccattaacac ttccgtgtcc accgtggcat ctaccgtccc ccccatgtat
     2281 gcaggaaatc ttcggacaaa gccacttcca gatgacagct ttggtgccac tgagcagaag
     2341 tttgcttctt cgctagaaaa ctccgactcc ccagttagaa gcatcctgaa atcccaaggc
     2401 tggcagccct cggtcgaggg tgctgggagc aaagcaatgt tgagagaatt tgaagagaca
     2461 gaacgcaagg gaggcttgac aggtggagat ggcggggtta caaagtatgg gtccttcgag
     2521 gaagcagagc tgtcctaccc cgtcctcagc agagtccggg agggagacaa ccataaagag
     2581 gccatctatg cccttccgag gaaaggaagc ctggagctcg cccatcctcc catcgcccag
     2641 cttggtgatg acctgaagga attttccacc ccaaagagca ccatgcaggc aagcccagac
     2701 tggaaggaga ggcagctctt tgaagagaag gtggacttgg agaatgtcac aaagaggaag
     2761 ttttcgctga aagccgcgga gttcggggaa cccacttctg agcagaccgg cgcggctgct
     2821 gggaaaccgg ctgctccgac tgcaaccccg gtgtcctgga agccgcagga tccctccgaa
     2881 cagcctcagg agaagcggta tcagagcccg tgtgcgatgt ttgctgctgg agagatcaaa
     2941 gccccggcgg tggagggcag cctcgactcc cccagcaaaa ccatgtccat taaagaaaga
     3001 ttagcactgt tgaagaagag cggtgaggaa gactggagaa acagactcaa cagaaagcag
     3061 gaatacggca aagcctccat caccagcagc ctgcacatcc aagagacgga gcagtcactc
     3121 aagaagaagc gggtcacaga aagtcgagag agccagatga ctattgaaga gagaaagcat
     3181 ctcatcaccg tgagagagga cgcctggaag accaggggca aaggagcagc caacgactct
     3241 acccagttta ctgtggccgg caggatggtg aagcgaggtc tggcgtcccc cactgccatc
     3301 accccggtag catcccctgt ttccagcaaa gcaaggggca ctacaccagt ttccagaccc
     3361 ctggaagata tcgaagccag accagacatg cagttagagt cggacctcaa gctggacagg
     3421 ctggaaacct tcctaagaag gctgaataac aaagttggtg ggatgcaaga aacagtcctc
     3481 actgtcactg ggaaatcggt gaaagaggtg atgaagccgg acgatgatga aacctttgcc
     3541 aagttttacc gcagcgtgga ctcctctcta ccgagaagcc ccgtagagct ggacgaggat
     3601 ttcgatgtca tttttgatcc ttatgccccc aggttgacat cctcggtggc tgagcacaag
     3661 cgtgccgtgc ggcccaagcg ccgggtccag gcttccaaaa accccctgaa gatgctggcc
     3721 gcgagggaag atctcctgca ggagtacact gagcagagac tgaacgtggc cttcgtggag
     3781 tcaaagcgga tgaaagtcga aaagttgtcc gccaactcca gcttctcaga agtcaccctg
     3841 gcggggttag ccagcaaaga aaacttcagc aacgtcagcc tgcggagcgt caacctgacg
     3901 gaacagaatt ccaacaacag tgcagtgccc tacaagaagc tgatgctgct gcaggttaaa
     3961 ggaagaagac acgtgcagac gcggctggtg gagcctcgcg ccccctccct caacagcggg
     4021 gactgcttcc tcctgctctc gccccatcac tgcttcctgt gggtcggaga gttcgccaac
     4081 gtgatcgaga aggcgaaggc ctcagaactt gcgagtttaa ttcagaccaa gagggaactt
     4141 ggttgtagag caacttacat ccagactgtt gaagaaggaa ttaatacaca cacacatgca
     4201 gccaaagact tctggaagct tctgggcggc caagccagtt accaatccgc cggagaccca
     4261 aaggaggatg agctctatga aacggccata atagagacca actgcattta ccgtctgatg
     4321 gatgacaaac tcgttcctga tgatgactac tgggggaaga tcccaaagtg ctccctcctg
     4381 caatcaaaag aggtactggt gtttgatttt gggagtgaag tttacgtgtg gcatggaaaa
     4441 gaagtcacat tagcacaacg gaaaatcgcc tttcagctgg caaagcactt gtggaatgga
     4501 acctttgact acgagaattg tgacattaac ccgctggatc ctggggaatg caatcccctc
     4561 attcccagga aaggacaggg gcggcccgac tgggcaatat ttgggagact tacagaacac
     4621 aatgagacga tcttgttcaa agagaaattc cttgattgga cggaactgaa gagacctaac
     4681 gagaagaacg ccagcgaact cgcgcagcac aaggacgatg cccgggcaga ggtcaagcct
     4741 tacgacgtga cgcggatggt gcccgtgccc cagacaacag ccggcaccgt gctggatggg
     4801 gtcaacgtgg gccgaggcta cgggctggtg gaaggggacg accggaggca gttcgagatc
     4861 gccagcatct cagtggacgt ctggcacatc ctggagttcg actacagcag gctccccaag
     4921 cagagcatcg ggcagttcca cgaaggcgat gcctacgtgg tgaagtggaa gttcatcgtg
     4981 agcactgcag tgggaagccg gcagaagggg gagcactcgg tcagggtggc tggcaaagag
     5041 aaatgtgtct acttcttctg gcaaggccgc cagtcgaccg tgagtgagaa gggcacatca
     5101 gctctgatga cagtggagct ggacgaggaa aggggggccc aggtccaggt cctgcagggc
     5161 aaggagcccc cctgtttcct gcagtgcttc caggggggga tggtggtcca ctccgggaga
     5221 cgggaggagg aggaagagaa cacacagagc gagtggcggc tgtactgtgt gcgcggagag
     5281 gtgcctgtgg aggggaactt gctggaggtg gcctgtcact gcagcagcct caggtctagg
     5341 acgtccatgg ttgtcctcaa cgtccacaaa gccctcatct acctgtggca tggatgcaaa
     5401 gcccaggccc acacgaagga ggtcggacgg actgcagcca ataaaatcaa ggaccaatgt
     5461 cccctggagg ccgggctgca cagtagcagc aaagtgacaa ttcatgagtg tgatgaaggc
     5521 tcggagcccc tggggttctg ggacgctctg gggaggagag accggaaagc ctacgactgc
     5581 atgcttcaag atcctggaaa ttttaacttc acaccccgcc tgttcatcct cagcagctcc
     5641 tctggtgact tctcagccac agagttcatg tatcccgccc gagacccctc tgtggtcaac
     5701 tctatgccct tcctgcagga agacctatac agtgccccac agccagcact cttccttgtt
     5761 gacaatcacc acgaggtgta cctctggcaa ggctggtggc ccatcgagaa caagatcacg
     5821 ggctccgcgc gcatccgctg ggcctcggac cggaagagcg ccatggagac agtgctgcag
     5881 tactgccgag ggaaaaacct gaagaagccg ccccccaagt cttacctcat ccacgctggc
     5941 ctggaacccc tgaccttcac caacatgttc cccagctggg agcacagaga agacattgca
     6001 gagatcacgg agatggacac ggaagtttca aatcagatca ccctagtgga ggatgtctta
     6061 gccaaactgt gtaagaccat ttacccactg gccgacctcc tagccaggcc gctccctgag
     6121 ggagttgacc ctctgaagct ggagatctat ctcaccgacg aggacttcga gtttgcacta
     6181 gacatgaccc gagacgagta caatgcactg cccgcttgga agcaggtgaa cctgaagaaa
     6241 gcgaaaggtc tgttctgagt caggagatgc tggaggaacc tgggtggtca ctttcaatac
     6301 tgatggacca ggaaaatgga tgtttaatcg aattcccgcg gccttctaga taactgatca
     6361 taatcagcca taccac
//
