LOCUS       sequence-84410-e        1136 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1136)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Talin-IBS2-7
FEATURES             Location/Qualifiers
     source          1..1136
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        81..132
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     83..103
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      161..170
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      257..266
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(263..265,266..268,269..982)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(308..329)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(569..588)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     916..937
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaaatgtc
       61 gtaacaactc cgcccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      121 ataagcagag ctggtttagt gaaccgtcag atccgctagc gccaccatgg gccaggtggt
      181 gctgatcaac gccgtgaagg acgtggccaa ggccctgggc gacctgatca gcgccaccaa
      241 gggggatcca ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
      301 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
      361 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
      421 gctgcccgtg ccctggccca ccctcgtgac caccttgacc tacggcgtgc agtgcttcgc
      481 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
      541 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
      601 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
      661 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca aggtctatat
      721 caccgccgac aagcagaaga acggcatcaa ggtgaacttc aagacccgcc acaacatcga
      781 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
      841 cgtgctgctg cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa
      901 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
      961 catggacgag ctgtacaagt aagcggccgc gactctagat cataatcagc cataccacat
     1021 ttgtagaggt ttttacttgc tttaaaaaaa cctcccacac ctcccccctg aacctgaaca
     1081 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gtttac
//
