LOCUS       sequence-84412-e        2660 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2660)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-TBRII-C-18
FEATURES             Location/Qualifiers
     source          1..2660
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      47..56
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(53..55,56..58,59..769)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(98..119)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(359..378)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     706..727
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2151..2162
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 aagcagtggt taagtgaaac gtcagatccc gctagcgcta ccggtcgcca ccatggtgag
       61 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
      121 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
      181 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
      241 caccttgacc tacggcgtgc agtgcttcgc ccgctacccc gaccacatga agcagcacga
      301 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
      361 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
      421 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
      481 gtacaactac aacagccaca aggtctatat caccgccgac aagcagaaga acggcatcaa
      541 ggtgaacttc aagacccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
      601 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
      661 cacccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
      721 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt ccggactcag
      781 atctcgagag agtggctctg gtgggtcgag tggaagtggc agcggtcggg ggctgctcag
      841 gggcctgtgg ccgctgcaca tcgtcctgtg gacgcgtatc gccagcacga tcccaccgca
      901 cgttcagaag tcggatgtgg aaatggaggc ccagaaagat gaaatcatct gccccagctg
      961 taataggact gcccatccac tgagacatat taataacgac atgatagtca ctgacaacaa
     1021 cggtgcagtc aagtttccac aactgtgtaa attttgtgat gtgagatttt ccacctgtga
     1081 caaccagaaa tcctgcatga gcaactgcag catcacctcc atctgtgaga agccacagga
     1141 agtctgtgtg gctgtatgga gaaagaatga cgagaacata acactagaga cagtttgcca
     1201 tgaccccaag ctcccctacc atgactttat tctggaagat gctgcttctc caaagtgcat
     1261 tatgaaggaa aaaaaaaagc ctggtgagac tttcttcatg tgttcctgta gctctgatga
     1321 gtgcaatgac aacatcatct tctcagaaga atataacacc agcaatcctg acttgttgct
     1381 agtcatattt caagtgacag gcatcagcct cctgccacca ctgggagttg ccatatctgt
     1441 catcatcatc ttctactgct accgcgttaa ccggcagcag aagctgagtt caacctggga
     1501 aaccggcaag acgcggaagc tcatggagtt cagcgagcac tgtgccatca tcctggaaga
     1561 tgaccgctct gacatcagct ccacgtgtgc caacaacatc aaccacaaca cagagctgct
     1621 gcccattgag ctggacaccc tggtggggaa aggtcgcttt gctgaggtct ataaggccaa
     1681 gctgaagcag aacacttcag agcagtttga gacagtggca gtcaagatct ttccctatga
     1741 ggagtatgcc tcttggaaga cagagaagga catcttctca gacatcaatc tgaagcatga
     1801 gaacatactc cagttcctga cggctgagga gcggaagacg gagttgggga aacaatactg
     1861 gctgatcacc gccttccacg ccaagggcaa cctacaggag tacctgacgc ggcatgtcat
     1921 cagctgggag gacctgcgca agctgggcag ctccctcgcc cgggggattg ctaacctcca
     1981 cagtgatcac actccatgtg ggaggcccaa gatgcccatc gtgcacaggg acctcaagag
     2041 ctccaatatc ctcgtgaaga acgacctaac ctgctgcctg tgtgactttg ggctttccct
     2101 gcgtctggac cctactctgt ctgtggatga cctggctaac agtgggcagg tgggaactgc
     2161 aagatacatg gctccagaag tcctagaatc caggatgaat ttggagaatg ttgagtcctt
     2221 caagcagacc gatgtctact ccatggctct ggtgctctgg gaaatgacat ctcgctgtaa
     2281 tgcagtggga gaagtaaaag attatgagcc tccatttggt tccaaggtgc gggagcaccc
     2341 ctgtgtcgaa agcatgaagg acaacgtgtt gagagatcga gggcgaccag aaattcccag
     2401 cttctggctc aaccaccagg gcatccagat ggtgtgtgag acgttgactg agtgctggga
     2461 ccacgaccca gaggcccgtc tcacagccca gtgtgtggca gaacgcttca gtgagctgga
     2521 gcatctggac aggctctcgg ggaggagctg ctcggaggag aagattcctg aagacggctc
     2581 cctaaacact accaaatagg gatccaccgg atctagataa ctgatcataa tcagccatac
     2641 cacattgtag agattttcac
//
