LOCUS       sequence-84413-e        2637 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2637)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-TBRII-N-18
FEATURES             Location/Qualifiers
     source          1..2637
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      31..40
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1367..1378
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     regulatory      1861..1870
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1867..1869,1870..1872,1873..2586)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1912..1933)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2173..2192)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2520..2541
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggccatgcta gtgacgtcag atccgctagc gccaccatgg gtcgggggct gctcaggggc
       61 ctgtggccgc tgcacatcgt cctgtggacg cgtatcgcca gcacgatccc accgcacgtt
      121 cagaagtcgg atgtggaaat ggaggcccag aaagatgaaa tcatctgccc cagctgtaat
      181 aggactgccc atccactgag acatattaat aacgacatga tagtcactga caacaacggt
      241 gcagtcaagt ttccacaact gtgtaaattt tgtgatgtga gattttccac ctgtgacaac
      301 cagaaatcct gcatgagcaa ctgcagcatc acctccatct gtgagaagcc acaggaagtc
      361 tgtgtggctg tatggagaaa gaatgacgag aacataacac tagagacagt ttgccatgac
      421 cccaagctcc cctaccatga ctttattctg gaagatgctg cttctccaaa gtgcattatg
      481 aaggaaaaaa aaaagcctgg tgagactttc ttcatgtgtt cctgtagctc tgatgagtgc
      541 aatgacaaca tcatcttctc agaagaatat aacaccagca atcctgactt gttgctagtc
      601 atatttcaag tgacaggcat cagcctcctg ccaccactgg gagttgccat atctgtcatc
      661 atcatcttct actgctaccg cgttaaccgg cagcagaagc tgagttcaac ctgggaaacc
      721 ggcaagacgc ggaagctcat ggagttcagc gagcactgtg ccatcatcct ggaagatgac
      781 cgctctgaca tcagctccac gtgtgccaac aacatcaacc acaacacaga gctgctgccc
      841 attgagctgg acaccctggt ggggaaaggt cgctttgctg aggtctataa ggccaagctg
      901 aagcagaaca cttcagagca gtttgagaca gtggcagtca agatctttcc ctatgaggag
      961 tatgcctctt ggaagacaga gaaggacatc ttctcagaca tcaatctgaa gcatgagaac
     1021 atactccagt tcctgacggc tgaggagcgg aagacggagt tggggaaaca atactggctg
     1081 atcaccgcct tccacgccaa gggcaaccta caggagtacc tgacgcggca tgtcatcagc
     1141 tgggaggacc tgcgcaagct gggcagctcc ctcgcccggg ggattgctaa cctccacagt
     1201 gatcacactc catgtgggag gcccaagatg cccatcgtgc acagggacct caagagctcc
     1261 aatatcctcg tgaagaacga cctaacctgc tgcctgtgtg actttgggct ttccctgcgt
     1321 ctggacccta ctctgtctgt ggatgacctg gctaacagtg ggcaggtggg aactgcaaga
     1381 tacatggctc cagaagtcct agaatccagg atgaatttgg agaatgttga gtccttcaag
     1441 cagaccgatg tctactccat ggctctggtg ctctgggaaa tgacatctcg ctgtaatgca
     1501 gtgggagaag taaaagatta tgagcctcca tttggttcca aggtgcggga gcacccctgt
     1561 gtcgaaagca tgaaggacaa cgtgttgaga gatcgagggc gaccagaaat tcccagcttc
     1621 tggctcaacc accagggcat ccagatggtg tgtgagacgt tgactgagtg ctgggaccac
     1681 gacccagagg cccgtctcac agcccagtgt gtggcagaac gcttcagtga gctggagcat
     1741 ctggacaggc tctcggggag gagctgctcg gaggagaaga ttcctgaaga cggctcccta
     1801 aacactacca aaagcagcgg tggagcaagc gcagccagtg gtagcgcgga tccaccggtc
     1861 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     1921 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     1981 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     2041 cccaccctcg tgaccacctt gacctacggc gtgcagtgct tcgcccgcta ccccgaccac
     2101 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     2161 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     2221 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     2281 gggcacaagc tggagtacaa ctacaacagc cacaaggtct atatcaccgc cgacaagcag
     2341 aagaacggca tcaaggtgaa cttcaagacc cgccacaaca tcgaggacgg cagcgtgcag
     2401 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     2461 aaccactacc tgagcaccca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac
     2521 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     2581 aagtaagcgg ccgcgactct agatcataat cagccatacc acattgtaga gatgcac
//
