LOCUS       sequence-84416-e        2114 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2114)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-TES-C-14
FEATURES             Location/Qualifiers
     source          1..2114
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      32..41
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(38..40,41..43,44..754)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(83..104)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(344..363)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     691..712
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agtgacgtca gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct
       61 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
      121 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
      181 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tgacctacgg
      241 cgtgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
      301 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
      361 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
      421 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
      481 ccacaaggtc tatatcaccg ccgacaagca gaagaacggc atcaaggtga acttcaagac
      541 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
      601 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct
      661 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
      721 cgggatcact ctcggcatgg acgagctgta caagtccgga ctcagatctg gcagcggtgg
      781 aggcagcgca tccggcgacc tggaaaacaa agtgaagaag atgggcttag gtcacgagca
      841 aggatttgga gccccttgtt taaaatgcaa agaaaaatgt gaaggattcg aactgcactt
      901 ctggagaaaa atatgtcgta actgcaagtg tggccaagaa gagcatgatg tcctcttgag
      961 caatgaagag gatcgaaaag tgggaaaact ttttgaagac accaagtata ccactctgat
     1021 tgcaaaacta aagtcagatg gaattcccat gtataaacgc aatgttatga tattgacgaa
     1081 tccagttgct gccaagaaga atgtctccat caatacagtt acctatgagt gggctcctcc
     1141 tgtccagaat caagcattgg ccaggcagta catgcagatg ctacccaagg aaaagcagcc
     1201 agtagcaggc tcagaggggg cacagtaccg gaagaagcag ctggcaaagc agctccctgc
     1261 acatgaccag gacccttcaa agtgccatga gttgtctccc agagaggtga aggagatgga
     1321 gcagtttgtg aagaaatata agagcgaagc tctgggagta ggagatgtca aacttccctg
     1381 tgagatggat gcccaaggcc ccaaacaaat gaacattcct ggaggggata gaagcacccc
     1441 agcagcagtg ggggccatgg aggacaaatc tgctgagcac aaaagaactc aatattcctg
     1501 ctattgctgc aaactgagta tgaaagaagg tgacccagcc atctatgccg aaagggctgg
     1561 ctatgataaa ctgtggcacc cagcttgttt tgtctgcagc acctgccatg aactcctggt
     1621 tgacatgatt tatttttgga agaatgagaa gctatactgt ggcagacatt actgtgacag
     1681 cgagaaaccc cgatgtgctg gctgtgacga gctgatattc agcaatgagt atacccaggc
     1741 agaaaaccag aattggcacc tgaaacactt ctgctgcttt gactgtgata gcattctagc
     1801 tggggagata tacgtgatgg tcaatgacaa gcccgtgtgc aagccctgct atgtgaagaa
     1861 tcacgctgtg gtgtgtcaag gatgccacaa tgccatcgac ccagaagtgc agcgggtgac
     1921 ctataacaat ttcagctggc atgcatccac agagtgcttt ctgtgctctt gctgcagcaa
     1981 atgcctcatt gggcagaagt tcatgccagt agaagggatg gttttctgtt cagtggaatg
     2041 taagaagagg atgtcttagg gatccaccgg atctagataa ctgatcataa tcagccatac
     2101 cacattttag gagg
//
