LOCUS       sequence-84417-e        2105 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2105)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-TES-N-14
FEATURES             Location/Qualifiers
     source          1..2105
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1328..1337
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1334..1336,1337..1339,1340..2053)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1379..1400)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1640..1659)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1987..2008
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tttgaacgtc agatccgcta gcgccaccat ggacctggaa aacaaagtga agaagatggg
       61 cttaggtcac gagcaaggat ttggagcccc ttgtttaaaa tgcaaagaaa aatgtgaagg
      121 attcgaactg cacttctgga gaaaaatatg tcgtaactgc aagtgtggcc aagaagagca
      181 tgatgtcctc ttgagcaatg aagaggatcg aaaagtggga aaactttttg aagacaccaa
      241 gtataccact ctgattgcaa aactaaagtc agatggaatt cccatgtata aacgcaatgt
      301 tatgatattg acgaatccag ttgctgccaa gaagaatgtc tccatcaata cagttaccta
      361 tgagtgggct cctcctgtcc agaatcaagc attggccagg cagtacatgc agatgctacc
      421 caaggaaaag cagccagtag caggctcaga gggggcacag taccggaaga agcagctggc
      481 aaagcagctc cctgcacatg accaggaccc ttcaaagtgc catgagttgt ctcccagaga
      541 ggtgaaggag atggagcagt ttgtgaagaa atataagagc gaagctctgg gagtaggaga
      601 tgtcaaactt ccctgtgaga tggatgccca aggccccaaa caaatgaaca ttcctggagg
      661 ggatagaagc accccagcag cagtgggggc catggaggac aaatctgctg agcacaaaag
      721 aactcaatat tcctgctatt gctgcaaact gagtatgaaa gaaggtgacc cagccatcta
      781 tgccgaaagg gctggctatg ataaactgtg gcacccagct tgttttgtct gcagcacctg
      841 ccatgaactc ctggttgaca tgatttattt ttggaagaat gagaagctat actgtggcag
      901 acattactgt gacagcgaga aaccccgatg tgctggctgt gacgagctga tattcagcaa
      961 tgagtatacc caggcagaaa accagaattg gcacctgaaa cacttctgct gctttgactg
     1021 tgatagcatt ctagctgggg agatatacgt gatggtcaat gacaagcccg tgtgcaagcc
     1081 ctgctatgtg aagaatcacg ctgtggtgtg tcaaggatgc cacaatgcca tcgacccaga
     1141 agtgcagcgg gtgacctata acaatttcag ctggcatgca tccacagagt gctttctgtg
     1201 ctcttgctgc agcaaatgcc tcattgggca gaagttcatg ccagtagaag ggatggtttt
     1261 ctgttcagtg gaatgtaaga agaggatgtc ttccggcggt agcggcggta gcggggatcc
     1321 accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     1381 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     1441 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     1501 gccctggccc accctcgtga ccaccttgac ctacggcgtg cagtgcttcg cccgctaccc
     1561 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     1621 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     1681 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     1741 catcctgggg cacaagctgg agtacaacta caacagccac aaggtctata tcaccgccga
     1801 caagcagaag aacggcatca aggtgaactt caagacccgc cacaacatcg aggacggcag
     1861 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     1921 gcccgacaac cactacctga gcacccagtc caagctgagc aaagacccca acgagaagcg
     1981 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     2041 gctgtacaag taagcggccg cgactctaga tcataatcag ccataccaca ttgtaggagt
     2101 taccc
//
