LOCUS       sequence-84418-e        3132 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Transferrin Receptor, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3132)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-TFR-20
FEATURES             Location/Qualifiers
     source          1..3132
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(980..991)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      2375..2384
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2381..2383,2384..2386,2387..3100)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2426..2447)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2687..2706)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3034..3055
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gatccgctag cgctaccgga ctcagatctc gagcgccacc atgatggatc aagctagatc
       61 agcattctct aacttgtttg gtggagaacc attgtcatat acccggttca gcctggctcg
      121 gcaagtagat ggcgataaca gtcatgtgga gatgaaactt gctgtagatg aagaagaaaa
      181 tgctgacaat aacacaaagg ccaatgtcac aaaaccaaaa aggtgtagtg gaagtatctg
      241 ctatgggact attgctgtga tcgtcttttt cttgattgga tttatgattg gctacttggg
      301 ctattgtaaa ggggtagaac caaaaactga gtgtgagaga ctggcaggaa ccgagtctcc
      361 agtgagggag gagccaggag aggacttccc tgcagcacgt cgcttatatt gggatgacct
      421 gaagagaaag ttgtcggaga aactggacag cacagacttc accagcacca tcaagctgct
      481 gaatgaaaat tcatatgtcc ctcgtgaggc tggatctcaa aaagatgaaa atcttgcgtt
      541 gtatgttgaa aatcaatttc gtgaatttaa actcagcaaa gtctggcgtg atcaacattt
      601 tgttaagatt caggtcaaag acagcgctca aaactcggtg atcatagttg ataagaacgg
      661 tagacttgtt tacctggtgg agaatcctgg gggttatgtg gcgtatagta aggctgcaac
      721 agttactggt aaactggtcc atgctaattt tggtactaaa aaagattttg aggatttata
      781 cactcctgtg aatggatcta tagtgattgt cagagcaggg aaaatcacct ttgcagaaaa
      841 ggttgcaaat gctgaaagct taaatgcaat tggtgtgttg atatacatgg accagactaa
      901 atttcccatt gttaacgcag aactttcatt ctttggacat gctcatctgg ggacaggtga
      961 cccttacaca cctggattcc cttccttcaa tcacactcag tttccaccat ctcggtcatc
     1021 aggattgcct aatatacctg tccagacaat ctccagagct gctgcagaaa agctgtttgg
     1081 gaatatggaa ggagactgtc cctctgactg gaaaacagac tctacatgta ggatggtaac
     1141 ctcagaaagc aagaatgtga agctcactgt gagcaatgtg ctgaaagaga taaaaattct
     1201 taacatcttt ggagttatta aaggctttgt agaaccagat cactatgttg tagttggggc
     1261 ccagagagat gcatggggcc ctggagctgc aaaatccggt gtaggcacag ctctcctatt
     1321 gaaacttgcc cagatgttct cagatatggt cttaaaagat gggtttcagc ccagcagaag
     1381 cattatcttt gccagttgga gtgctggaga ctttggatcg gttggtgcca ctgaatggct
     1441 agagggatac ctttcgtccc tgcatttaaa ggctttcact tatattaatc tggataaagc
     1501 ggttcttggt accagcaact tcaaggtttc tgccagccca ctgttgtata cgcttattga
     1561 gaaaacaatg caaaatgtga agcatccggt tactgggcaa tttctatatc aggacagcaa
     1621 ctgggccagc aaagttgaga aactcacttt agacaatgct gctttccctt tccttgcata
     1681 ttctggaatc ccagcagttt ctttctgttt ttgcgaggac acagattatc cttatttggg
     1741 taccaccatg gacacctata aggaactgat tgagaggatt cctgagttga acaaagtggc
     1801 acgagcagct gcagaggtcg ctggtcagtt cgtgattaaa ctaacccatg atgttgaatt
     1861 gaacctggac tatgagaggt acaacagcca actgctttca tttgtgaggg atctgaacca
     1921 atacagagca gacataaagg aaatgggcct gagtttacag tggctgtatt ctgctcgtgg
     1981 agacttcttc cgtgctactt ccagactaac aacagatttc gggaatgctg agaaaacaga
     2041 cagatttgtc atgaagaaac tcaatgatcg tgtcatgaga gtggagtatc acttcctctc
     2101 tccctacgta tctccaaaag agtctccttt ccgacatgtc ttctggggct ccggctctca
     2161 cacgctgcca gctttactgg agaacttgaa actgcgtaaa caaaataacg gtgcttttaa
     2221 tgaaacgctg ttcagaaacc agttggctct agctacttgg actattcagg gagctgcaaa
     2281 tgccctctct ggtgacgttt gggacattga caatgagttt tcagaattcg gctccaccgg
     2341 ctccaccggc tccaccggcg cggatccacc ggtcgccacc atggtgagca agggcgagga
     2401 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     2461 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     2521 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttgaccta
     2581 cggcgtgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     2641 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     2701 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     2761 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     2821 cagccacaag gtctatatca ccgccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     2881 gacccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     2941 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccaa
     3001 gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     3061 cgccgggatc actctcggca tggacgagct gtacaagtaa gcggccgcga ctctagatca
     3121 taatcagcca ta
//
