LOCUS       sequence-84419-e        2127 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi Complex, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2127)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-TGNP-N-10
FEATURES             Location/Qualifiers
     source          1..2127
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1363..1372
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1369..1371,1372..1374,1375..2088)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1414..1435)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1675..1694)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2022..2043
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acggtcagat ccgctagcta tgccaccatg cggttcgtgg ttgccttggt cctcctgaac
       61 gtcgcagcgg cgggagccgt gccgctcttg gccaccgaaa gcgtcaagca agaagaagct
      121 ggagtacggc cttctgcagg aaacgtctcc acccacccca gcttgagcca acggcctgga
      181 ggctctacca agtcgcatcc ggagccgcag actccaaaag acagccctag caagtcgagt
      241 gcggaggcgc agaccccaga agacaccccc aacaagtcgg gtgcggaggc aaagacccaa
      301 aaagacagct ccaacaagtc gggtgcggag gcaaagaccc aaaaaggcag cactagcaag
      361 tcgggttcgg aggcgcagac cacaaaagac agcactagta agtcgcatcc ggagctgcag
      421 actccaaaag acagcactgg caaatcgggt gcggaggcgc agaccccaga agacagcccc
      481 aacaggtcgg gtgcggaggc aaagacccaa aaagacagcc ctagcaagtc aggttcggag
      541 gcgcagacca caaaagatgt ccctaataag tcgggtgcgg acggccagac cccaaaagac
      601 ggctccagca agtcgggtgc ggaggatcag accccaaaag acgtccctaa caagtcgggt
      661 gcggagaagc agactccaaa agacggctct aacaagtccg gtgcagagga gcagggccca
      721 atagacgggc ccagcaagtc gggtgcggag gagcagacct caaaagacag ccctaacaag
      781 gtggttccag agcagccttc ctggaaagac cattccaagc ccatctccaa cccttctgat
      841 aacaaggagc tccccaaggc tgacacaaac cagcttgctg acaaagggaa gctttctcct
      901 catgctttca aaaccgaatc tggggaggaa actgacctca tttctccccc gcaggaggaa
      961 gttaagtctt cagagcctac tgaggatgtg gagcccaaag aggctgaaga tgatgataca
     1021 ggacccgagg agggctcacc gcccaaagaa gagaaagaaa agatgtccgg ttctgcctcc
     1081 agtgagaacc gtgaaggaac actttcggat tccacgggta gcgagaagga tgacctttat
     1141 ccgaacggtt ctggaaatgg cagcgcggag agcagccact tctttgcata tctggtgact
     1201 gcagccattc ttgtggctgt cctctatatc gctcatcaca acaagcggaa gatcattgct
     1261 tttgtcctgg aaggaaaaag atctaaagtc acccggcggc caaaggccag tgactaccaa
     1321 cgtttggacc agaagtccag ctccggaggg gatccaccgg tcgccaccat ggtgagcaag
     1381 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     1441 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     1501 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1561 ttgacctacg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc
     1621 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1681 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1741 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1801 aactacaaca gccacaaggt ctatatcacc gccgacaagc agaagaacgg catcaaggtg
     1861 aacttcaaga cccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1921 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1981 cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     2041 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaagc ggccgcgact
     2101 ctagatcata atcagccata ccacatt
//
