LOCUS       sequence-84422-e        1369 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubule Associated Protein, Excitation: 487, 
            Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1369)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-TPT1-C-10
FEATURES             Location/Qualifiers
     source          1..1369
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(28..30,31..33,34..744)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(73..94)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(334..353)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     681..702
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
       61 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      121 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      181 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tgacctacgg cgtgcagtgc
      241 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      301 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      361 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      421 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaaggtc
      481 tatatcaccg ccgacaagca gaagaacggc atcaaggtga acttcaagac ccgccacaac
      541 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      601 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac
      661 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
      721 ctcggcatgg acgagctgta caagtccgga ctcagatctg gcagcagtgg aggtattatc
      781 taccgggacc tcatcagcca cgatgagatg ttctccgaca tctacaagat ccgggagatc
      841 gcggacgggt tgtgcctgga ggtggagggg aagatggtca gtaggacaga aggtaacatt
      901 gatgactcgc tcattggtgg aaatgcctcc gctgaaggcc ccgagggcga aggtaccgaa
      961 agcacagtaa tcactggtgt cgatattgtc atgaaccatc acctgcagga aacaagtttc
     1021 acaaaagaag cctacaagaa gtacatcaaa gattacatga aatcaatcaa agggaaactt
     1081 gaagaacaga gaccagaaag agtaaaacct tttatgacag gggctgcaga acaaatcaag
     1141 cacatccttg ctaatttcaa aaactaccag ttctttattg gtgaaaacat gaatccagat
     1201 ggcatggttg ctctattgga ctaccgtgag gatggtgtga ccccatatat gattttcttt
     1261 aaggatggtt tagaaatgga aaaatgttaa gaattctgca gtcgacggta ccgcgggccc
     1321 gggatccacc ggatctagat aactgatcat aatcagccat accacattt
//
