LOCUS       sequence-84423-e        3047 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Centrosomes, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3047)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-TPX2-N-10
FEATURES             Location/Qualifiers
     source          1..3047
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2290..2299
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2296..2298,2299..2301,2302..3015)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2341..2362)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2602..2621)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2949..2970
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagccgc caccatgtca caagttaaaa gctcttattc ctatgatgcc
       61 ccctcggatt tcatcaattt ttcatccttg gatgatgaag gagatactca aaacatagat
      121 tcatggtttg aggagaaggc caatttggag aataagttac tggggaagaa tggaactgga
      181 gggctttttc agggcaaaac tcctttgaga aaggctaatc ttcagcaagc tattgtcaca
      241 cctttgaaac cagttgacaa cacttactac aaagaggcag aaaaagaaaa tcttgtggaa
      301 caatccattc cgtcaaatgc ttgttcttcc ctggaagttg aggcagccat atcaagaaaa
      361 actccagccc agcctcagag aagatctctt aggctttctg ctcagaagga tttggaacag
      421 aaagaaaagc atcatgtaaa aatgaaagcc aagagatgtg ccactcctgt aatcatcgat
      481 gaaattctac cctctaagaa aatgaaagtt tctaacaaca aaaagaagcc agaggaagaa
      541 ggcagtgctc atcaagatac tgctgaaaag aatgcatctt ccccagagaa agccaagggt
      601 agacatactg tgccttgtat gccacctgca aagcagaagt ttctaaaaag tactgaggag
      661 caagagctgg agaagagtat gaaaatgcag caagaggtgg tggagatgcg gaaaaagaat
      721 gaagaattca agaaacttgc tctggctgga atagggcaac ctgtgaagaa atcagtgagc
      781 caggtcacca aatcagttga cttccacttc cgcacagatg agcgaatcaa acaacatcct
      841 aagaaccagg aggaatataa ggaagtgaac tttacatctg aactacgaaa gcatccttca
      901 tctcctgccc gagtgactaa gggatgtacc attgttaagc ctttcaacct gtcccaagga
      961 aagaaaagaa catttgatga aacagtttct acatatgtgc cccttgcaca gcaagttgaa
     1021 gacttccata aacgaacccc taacagatat catttgagga gcaagaagga tgatattaac
     1081 ctgttaccct ccaaatcttc tgtgaccaag atttgcagag acccacagac tcctgtactg
     1141 caaaccaaac accgtgcacg ggctgtgacc tgcaaaagta cagcagagct ggaggctgag
     1201 gagctcgaga aattgcaaca atacaaattc aaagcacgtg aacttgatcc cagaatactt
     1261 gaaggtgggc ccatcttgcc caagaaacca cctgtgaaac cacccaccga gcctattggc
     1321 tttgatttgg aaattgagaa aagaatccag gagcgagaat caaagaagaa aacagaggat
     1381 gaacactttg aatttcattc cagaccttgc cctactaaga ttttggaaga tgttgtgggt
     1441 gttcctgaaa agaaggtact tccaatcacc gtccccaagt caccagcctt tgcattgaag
     1501 aacagaattc gaatgcccac caaagaagat gaggaagagg acgaaccggt agtgataaaa
     1561 gctcaacctg tgccacatta tggggtgcct tttaagcccc aaatcccaga ggcaagaact
     1621 gtggaaatat gccctttctc gtttgattct cgagacaaag aacgtcagtt acagaaggag
     1681 aagaaaataa aagaactgca gaaaggggag gtgcccaagt tcaaggcact tcccttgcct
     1741 cattttgaca ccattaacct gccagagaag aaggtaaaga atgtgaccca gattgaacct
     1801 ttctgcttgg agactgacag aagaggtgct ctgaaggcac agacttggaa gcaccagctg
     1861 gaagaagaac tgagacagca gaaagaagca gcttgtttca aggctcgtcc aaacaccgtc
     1921 atctctcagg agccctttgt tcccaagaaa gagaagaaat cagttgctga gggcctttct
     1981 ggttctctag ttcaggaacc ttttcagctg gctactgaga agagagccaa agagcggcag
     2041 gagctggaga agagaatggc tgaggtagaa gcccagaaag cccagcagtt ggaggaggcc
     2101 agactacagg aggaagagca gaaaaaagag gagctggcca ggctacggag agaactggtg
     2161 cataaggcaa atccaatacg caagtaccag ggtctggaga taaagtcaag tgaccagcct
     2221 ctgactgtgc ctgtatctcc caaattctcc actcgattcc actgctcggg aagcacggat
     2281 ccaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     2341 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     2401 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     2461 gtgccctggc ccaccctcgt gaccaccttg acctacggcg tgcagtgctt cgcccgctac
     2521 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     2581 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     2641 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     2701 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaaggtcta tatcaccgcc
     2761 gacaagcaga agaacggcat caaggtgaac ttcaagaccc gccacaacat cgaggacggc
     2821 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     2881 ctgcccgaca accactacct gagcacccag tccaagctga gcaaagaccc caacgagaag
     2941 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     3001 gagctgtaca agtaagcggc cgcgactcta gatcataatc agccata
//
