LOCUS       sequence-84424-e        1720 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1720)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Tropomyosin-C-14
FEATURES             Location/Qualifiers
     source          1..1720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(35..37,38..40,41..751)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(80..101)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(341..360)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     688..709
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt
       61 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      121 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      181 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttga cctacggcgt
      241 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      301 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      361 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      421 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
      481 caaggtctat atcaccgccg acaagcagaa gaacggcatc aaggtgaact tcaagacccg
      541 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      601 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag
      661 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
      721 gatcactctc ggcatggacg agctgtacaa gtccggactc agatctggtg gcagtggagg
      781 gtccggggga gctgacgcca tcaagaagaa gatgcagatg ctgaagctcg acaaggagaa
      841 cgccttggat cgagctgagc aggcggaggc cgacaagaag gcggcggaag acaggagcaa
      901 gcagctggaa gatgagctgg tgtcactgca aaagaaactc aagggcaccg aagatgaact
      961 ggacaaatac tctgaggctc tcaaagatgc ccaggagaag ctggagctgg cagagaaaaa
     1021 ggccaccgat gctgaagccg acgtagcttc tctgaacaga cgcatccagc tggttgagga
     1081 agagttggat cgtgcccagg agcgtctggc aacagctttg cagaagctgg aggaagctga
     1141 gaaggcagca gatgagagtg agagaggcat gaaagtcatt gagagtcgag cccaaaaaga
     1201 tgaagaaaaa atggaaattc aggagatcca actgaaagag gcaaagcaca ttgctgaaga
     1261 tgccgaccgc aaatatgaag aggtggcccg taagctggtc atcattgaga gcgacctgga
     1321 acgtgcagag gagcgggctg agctctcaga aggcaaatgt gccgagcttg aagaagaatt
     1381 gaaaactgtg acgaacaact tgaagtcact ggaggctcag gctgagaagt actcgcagaa
     1441 ggaagacaga tatgaggaag agatcaaggt cctttccgac aagctgaagg aggctgagac
     1501 tcgggctgag tttgcggaga ggtcagtaac taaattggag aaaagcattg atgacttaga
     1561 agacgagctg tacgctcaga aactgaagta caaagccatc agcgaggagc tggaccacgc
     1621 tctcaacgat atgacttcca tataagaatt ctgcagtcga cggtaccgcg ggcccgggat
     1681 ccaccggatc tagataactg atcataatca gccataccac
//
