LOCUS       sequence-84426-e        2176 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Day RN, Davidson MW
  TITLE     The fluorescent protein palette: tools for cellular imaging.
  JOURNAL   Chem Soc Rev. 2009 Oct;38(10):2887-921. doi: 10.1039/b901966a. Epub 
            2009 Aug 4.
  PUBMED    19771335
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1039/b901966a"; 
            journalName: "Chem Soc Rev"; date: "2009-10"; volume: "38"; issue: 
            "10"; pages: "2887-921"
COMMENT     Sequence Label: mEmerald-Tubulin-6
FEATURES             Location/Qualifiers
     source          1..2176
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(36..38,39..41,42..752)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(81..102)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(342..361)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     689..710
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tgacgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggagctgt
       61 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
      121 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
      181 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttg acctacggcg
      241 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
      301 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
      361 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
      421 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
      481 acaaggtcta tatcaccgcc gacaagcaga agaacggcat caaggtgaac ttcaagaccc
      541 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
      601 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga
      661 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
      721 ggatcactct cggcatggac gagctgtaca agtccggact cagatctcga gtgcgtgagt
      781 gcatctccat ccacgttggc caggctggtg tccagattgg caatgcctgc tgggagctct
      841 actgcctgga acacggcatc cagcccgatg gccagatgcc aagtgacaag accattgggg
      901 gaggagatga ctccttcaac accttcttca gtgagacggg cgctggcaag cacgtgcccc
      961 gggctgtgtt tgtagacttg gaacccacag tcattgatga agttcgcact ggcacctacc
     1021 gccagctctt ccaccctgag cagctcatca caggcaagga agatgctgcc aataactatg
     1081 cccgagggca ctacaccatt ggcaaggaga tcattgacct tgtgttggac cgaattcgca
     1141 agctggctga ccagtgcacc ggtcttcagg gcttcttggt tttccacagc tttggtgggg
     1201 gaactggttc tgggttcacc tccctgctca tggaacgtct ctcagttgat tatggcaaga
     1261 agtccaagct ggagttctcc atttacccag caccccaggt ttccacagct gtagttgagc
     1321 cctacaactc catcctcacc acccacacca ccctggagca ctctgattgt gccttcatgg
     1381 tagacaatga ggccatctat gacatctgtc gtagaaacct cgatatcgag cgcccaacct
     1441 acactaacct taaccgcctt attagccaga ttgtgtcctc catcactgct tccctgagat
     1501 ttgatggagc cctgaatgtt gacctgacag aattccagac caacctggtg ccctaccccc
     1561 gcatccactt ccctctggcc acatatgccc ctgtcatctc tgctgagaaa gcctaccatg
     1621 aacagctttc tgtagcagag atcaccaatg cttgctttga gccagccaac cagatggtga
     1681 aatgtgaccc tcgccatggt aaatacatgg cttgctgcct gttgtaccgt ggtgacgtgg
     1741 ttcccaaaga tgtcaatgct gccattgcca ccatcaaaac caagcgcagc atccagtttg
     1801 tggattggtg ccccactggc ttcaaggttg gcatcaacta ccagcctccc actgtggtgc
     1861 ctggtggaga cctggccaag gtacagagag ctgtgtgcat gctgagcaac accacagcca
     1921 ttgctgaggc ctgggctcgc ctggaccaca agtttgacct gatgtatgcc aagcgtgcct
     1981 ttgttcactg gtacgtgggt gaggggatgg aggaaggcga gttttcagag gcccgtgaag
     2041 atatggctgc ccttgagaag gattatgagg aggttggtgt ggattctgtt gaaggagagg
     2101 gtgaggaaga aggagaggaa tactaaggat ccaccggatc tagataactg atcataatca
     2161 gccataccac attgag
//
