LOCUS       sequence-84428-e        2188 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2188)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Tubulin-N-18
FEATURES             Location/Qualifiers
     source          1..2188
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      16..25
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1426..1435
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1432..1434,1435..1437,1438..2151)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1477..1498)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1738..1757)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2085..2106
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtcagatccg ctagcgccac catggtgcgt gagtgcatct ccatccacgt tggccaggct
       61 ggtgtccaga ttggcaatgc ctgctgggag ctctactgcc tggaacacgg catccagccc
      121 gatggccaga tgccaagtga caagaccatt gggggaggag atgactcctt caacaccttc
      181 ttcagtgaga cgggcgctgg caagcacgtg ccccgggctg tgtttgtaga cttggaaccc
      241 acagtcattg atgaagttcg cactggcacc taccgccagc tcttccaccc tgagcagctc
      301 atcacaggca aggaagatgc tgccaataac tatgcccgag ggcactacac cattggcaag
      361 gagatcattg accttgtgtt ggaccgaatt cgcaagctgg ctgaccagtg caccggtctt
      421 cagggcttct tggttttcca cagctttggt gggggaactg gttctgggtt cacctccctg
      481 ctcatggaac gtctctcagt tgattatggc aagaagtcca agctggagtt ctccatttac
      541 ccagcacccc aggtttccac agctgtagtt gagccctaca actccatcct caccacccac
      601 accaccctgg agcactctga ttgtgccttc atggtagaca atgaggccat ctatgacatc
      661 tgtcgtagaa acctcgatat cgagcgccca acctacacta accttaaccg ccttattagc
      721 cagattgtgt cctccatcac tgcttccctg agatttgatg gagccctgaa tgttgacctg
      781 acagaattcc agaccaacct ggtgccctac ccccgcatcc acttccctct ggccacatat
      841 gcccctgtca tctctgctga gaaagcctac catgaacagc tttctgtagc agagatcacc
      901 aatgcttgct ttgagccagc caaccagatg gtgaaatgtg accctcgcca tggtaaatac
      961 atggcttgct gcctgttgta ccgtggtgac gtggttccca aagatgtcaa tgctgccatt
     1021 gccaccatca aaaccaagcg cagcatccag tttgtggatt ggtgccccac tggcttcaag
     1081 gttggcatca actaccagcc tcccactgtg gtgcctggtg gagacctggc caaggtacag
     1141 agagctgtgt gcatgctgag caacaccaca gccattgctg aggcctgggc tcgcctggac
     1201 cacaagtttg acctgatgta tgccaagcgt gcctttgttc actggtacgt gggtgagggg
     1261 atggaggaag gcgagttttc agaggcccgt gaagatatgg ctgcccttga gaaggattat
     1321 gaggaggttg gtgtggattc tgttgaagga gagggtgagg aagaaggaga ggaatacagc
     1381 agcggtggag caagcgcagc cagtggtagc gcggatccac cggtcgccac catggtgagc
     1441 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     1501 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     1561 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     1621 accttgacct acggcgtgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac
     1681 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     1741 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     1801 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     1861 tacaactaca acagccacaa ggtctatatc accgccgaca agcagaagaa cggcatcaag
     1921 gtgaacttca agacccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     1981 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     2041 acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     2101 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta agcggccgcg
     2161 actctagatc ataatcagcc ataccaca
//
