LOCUS       sequence-84429-e        1917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin/Focal Adhesions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1917)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-VASP-5
FEATURES             Location/Qualifiers
     source          1..1917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(30..32,33..35,36..746)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(75..96)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(336..355)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     683..704
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cagatccgct agcgctaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg
       61 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
      121 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
      181 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttgacctac ggcgtgcagt
      241 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
      301 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
      361 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
      421 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaagg
      481 tctatatcac cgccgacaag cagaagaacg gcatcaaggt gaacttcaag acccgccaca
      541 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
      601 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaag ctgagcaaag
      661 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
      721 ctctcggcat ggacgagctg tacaagtccg gactcagatc tatgagcgag acggtcatct
      781 gttccagccg ggctactgtg atgctttatg atgacagcaa caagcgatgg ctccctgctg
      841 gcactggtcc ccaggccttc agccgcgtcc agatatacca caaccccact gctaactcct
      901 tccgagttgt tggccgcaag atgcagccgg atcagcaggt ggttatcaac tgtgccatca
      961 ttcggggtgt caagtacaat caggccactc ccatcttcca tcagtggcga gatgcccgcc
     1021 aggtctgggg cctcaacttc ggcagcaagg aggacgccat acagtttgca acaggcatgg
     1081 ccaacgccct agaggccttg gaaggaggtg ggcctccccc agccccagca ccccctgcct
     1141 ggtctgccca gaatggtccc tccccagagg agctggaaca acagaaaagg cagccggagc
     1201 atatggagcg ccgggtctcc aatgcaggag gcccacctgc tcccccagct gggggccctc
     1261 ctccacctcc aggacctccc cctcctccag gtcccccccc acccccaggt ctgccctcct
     1321 caggggtatc tggggcaggt catggagcag gggcagcccc accccctgca cccccactcc
     1381 ctacaacaca gggccccaat agtgggggtt ccggggcccc aggcctggct gctgccattg
     1441 ctggagccaa actcaggaaa gtgagcaagc aggaggaggc ctctgggggg cccctggccc
     1501 ccaaagctga gaacagtcga agcactggtg gggggcttat ggaagagatg aacgccatgc
     1561 tggcccggag aagaaaagcc acacaggttg gggagaagcc ccccaaagac gagtcagcca
     1621 gtgaggagtc agaggcccga ctccctgccc agagtgaacc tgtgagaaga ccctgggaga
     1681 agaacagcac aaccttgcca aggatgaagt cgtcctcttc tgtgactacc tccgaggccc
     1741 acccctcgac gccctgctcc agtgatgact ccgacttgga gagggtgaag caggagcttc
     1801 tggaagaggt gcggaaggag ctacagaaaa tgaaagagga aatcatcgaa gtctttgtcc
     1861 aggagctgag gaagcggggt tctccttgag cggccgcgac tctagatcat aatcagc
//
