LOCUS       sequence-84430-e        1938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin/Focal Adhesions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1938)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-VASP-N-10
FEATURES             Location/Qualifiers
     source          1..1938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1174..1183
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1180..1182,1183..1185,1186..1899)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1225..1246)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1486..1505)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1833..1854
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtcagatccg ctagcgatgc caccatgagc gagacggtca tctgttccag ccgggctact
       61 gtgatgcttt atgatgacag caacaagcga tggctccctg ctggcactgg tccccaggcc
      121 ttcagccgcg tccagatata ccacaacccc actgctaact ccttccgagt tgttggccgc
      181 aagatgcagc cggatcagca ggtggttatc aactgtgcca tcattcgggg tgtcaagtac
      241 aatcaggcca ctcccatctt ccatcagtgg cgagatgccc gccaggtctg gggcctcaac
      301 ttcggcagca aggaggacgc catacagttt gcaacaggca tggccaacgc cctagaggcc
      361 ttggaaggag gtgggcctcc cccagcccca gcaccccctg cctggtctgc ccagaatggt
      421 ccctccccag aggagctgga acaacagaaa aggcagccgg agcatatgga gcgccgggtc
      481 tccaatgcag gaggcccacc tgctccccca gctgggggcc ctcctccacc tccaggacct
      541 ccccctcctc caggtccccc cccaccccca ggtctgccct cctcaggggt atctggggca
      601 ggtcatggag caggggcagc cccaccccct gcacccccac tccctacaac acagggcccc
      661 aatagtgggg gttccggggc cccaggcctg gctgctgcca ttgctggagc caaactcagg
      721 aaagtgagca agcaggagga ggcctctggg gggcccctgg cccccaaagc tgagaacagt
      781 cgaagcactg gtggggggct tatggaagag atgaacgcca tgctggcccg gagaagaaaa
      841 gccacacagg ttggggagaa gccccccaaa gacgagtcag ccagtgagga gtcagaggcc
      901 cgactccctg cccagagtga acctgtgaga agaccctggg agaagaacag cacaaccttg
      961 ccaaggatga agtcgtcctc ttctgtgact acctccgagg cccacccctc gacgccctgc
     1021 tccagtgatg actccgactt ggagagggtg aagcaggagc ttctggaaga ggtgcggaag
     1081 gagctacaga aaatgaaaga ggaaatcatc gaagtctttg tccaggagct gaggaagcgg
     1141 ggttctccta gctccggagg ggatccaccg gtcgccacca tggtgagcaa gggcgaggag
     1201 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     1261 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     1321 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttgacctac
     1381 ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     1441 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     1501 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     1561 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     1621 agccacaagg tctatatcac cgccgacaag cagaagaacg gcatcaaggt gaacttcaag
     1681 acccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     1741 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaag
     1801 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     1861 gccgggatca ctctcggcat ggacgagctg tacaagtaag cggccgcgac tctagatcat
     1921 aatcagccat accacatt
//
