LOCUS       sequence-84431-e        2242 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 487, Emission: 
            509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2242)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Vimentin-7
FEATURES             Location/Qualifiers
     source          1..2242
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1472..1481
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1478..1480,1481..1483,1484..2197)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1523..1544)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1784..1803)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2131..2152
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattcccat
       61 gtccaccagg tccgtgtcct cgtcctccta ccgcaggatg ttcggcggcc cgggcaccgc
      121 gagccggccg agctccagcc ggagctacgt gactacgtcc acccgcacct acagcctggg
      181 cagcgcgctg cgccccagca ccagccgcag cctctacgcc tcgtccccgg gcggcgtgta
      241 tgccacgcgc tcctctgccg tgcgcctgcg gagcagcgtg cccggggtgc ggctcctgca
      301 ggactcggtg gacttctcgc tggccgacgc catcaacacc gagttcaaga acacccgcac
      361 caacgagaag gtggagctgc aggagctgaa tgaccgcttc gccaactaca tcgacaaggt
      421 gcgcttcctg gagcagcaga ataagatcct gctggccgag ctcgagcagc tcaagggcca
      481 aggcaagtcg cgcctggggg acctctacga ggaggagatg cgggagctgc gccggcaggt
      541 ggaccagcta accaacgaca aagcccgcgt cgaggtggag cgcgacaacc tggccgagga
      601 catcatgcgc ctccgggaga aattgcagga ggagatgctt cagagagagg aagccgaaaa
      661 caccctgcaa tctttcagac aggatgttga caatgcgtct ctggcacgtc ttgaccttga
      721 acgcaaagtg gaatctttgc aagaagagat tgcctttttg aagaaactcc acgaagagga
      781 aatccaggag ctgcaggctc agattcagga acagcatgtc caaatcgatg tggatgtttc
      841 caagcctgac ctcacggctg ccctgcgtga cgtacgtcag caatatgaaa gtgtggctgc
      901 caagaacctg caggaggcag aagaatggta caaatccaag tttgctgacc tctctgaggc
      961 tgccaaccgg aacaatgacg ccctgcgcca ggcaaagcag gagtccactg agtaccggag
     1021 acaggtgcag tccctcacct gtgaagtgga tgcccttaaa ggaaccaatg agtccctgga
     1081 acgccagatg cgtgaaatgg aagagaactt tgccgttgaa gctgctaact accaagacac
     1141 tattggccgc ctgcaggatg agattcagaa tatgaaggag gaaatggctc gtcaccttcg
     1201 tgaataccaa gacctgctca atgttaagat ggcccttgac attgagattg ccacctacag
     1261 gaagctgctg gaaggcgagg agagcaggat ttctctgcct cttccaaact tttcctccct
     1321 gaacctgagg gaaactaatc tggattcact ccctctggtt gatacccact caaaaaggac
     1381 acttctgatt aagacggttg aaactagaga tggacaggtt atcaacgaaa cttctcagca
     1441 tcacgatgac cttgaagggg atccaccggt cgccaccatg gtgagcaagg gcgaggagct
     1501 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     1561 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     1621 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tgacctacgg
     1681 cgtgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1741 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1801 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1861 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1921 ccacaaggtc tatatcaccg ccgacaagca gaagaacggc atcaaggtga acttcaagac
     1981 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     2041 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct
     2101 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     2161 cgggatcact ctcggcatgg acgagctgta caagtaagcg gccgcgactc tagatcataa
     2221 tcagccatac cacattgaga gg
//
