LOCUS       sequence-84432-e        2247 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 487, Emission: 
            509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2247)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Vimentin-C-18
FEATURES             Location/Qualifiers
     source          1..2247
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(32..34,35..37,38..748)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(77..98)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(338..357)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     685..706
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtcagatccg ctagcgctac cggtcgccac catggtgagc aagggcgagg agctgttcac
       61 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      121 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
      181 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttgacct acggcgtgca
      241 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
      301 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
      361 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
      421 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
      481 ggtctatatc accgccgaca agcagaagaa cggcatcaag gtgaacttca agacccgcca
      541 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
      601 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa
      661 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
      721 cactctcggc atggacgagc tgtacaagtc cggactcaga tctggcagcg gtggaggcag
      781 cgcatccggc ggaagcggaa gctccaccag gtccgtgtcc tcgtcctcct accgcaggat
      841 gttcggcggc ccgggcaccg cgagccggcc gagctccagc cggagctacg tgactacgtc
      901 cacccgcacc tacagcctgg gcagcgcgct gcgccccagc accagccgca gcctctacgc
      961 ctcgtccccg ggcggcgtgt atgccacgcg ctcctctgcc gtgcgcctgc ggagcagcgt
     1021 gcccggggtg cggctcctgc aggactcggt ggacttctcg ctggccgacg ccatcaacac
     1081 cgagttcaag aacacccgca ccaacgagaa ggtggagctg caggagctga atgaccgctt
     1141 cgccaactac atcgacaagg tgcgcttcct ggagcagcag aataagatcc tgctggccga
     1201 gctcgagcag ctcaagggcc aaggcaagtc gcgcctgggg gacctctacg aggaggagat
     1261 gcgggagctg cgccggcagg tggaccagct aaccaacgac aaagcccgcg tcgaggtgga
     1321 gcgcgacaac ctggccgagg acatcatgcg cctccgggag aaattgcagg aggagatgct
     1381 tcagagagag gaagccgaaa acaccctgca atctttcaga caggatgttg acaatgcgtc
     1441 tctggcacgt cttgaccttg aacgcaaagt ggaatctttg caagaagaga ttgccttttt
     1501 gaagaaactc cacgaagagg aaatccagga gctgcaggct cagattcagg aacagcatgt
     1561 ccaaatcgat gtggatgttt ccaagcctga cctcacggct gccctgcgtg acgtacgtca
     1621 gcaatatgaa agtgtggctg ccaagaacct gcaggaggca gaagaatggt acaaatccaa
     1681 gtttgctgac ctctctgagg ctgccaaccg gaacaatgac gccctgcgcc aggcaaagca
     1741 ggagtccact gagtaccgga gacaggtgca gtccctcacc tgtgaagtgg atgcccttaa
     1801 aggaaccaat gagtccctgg aacgccagat gcgtgaaatg gaagagaact ttgccgttga
     1861 agctgctaac taccaagaca ctattggccg cctgcaggat gagattcaga atatgaagga
     1921 ggaaatggct cgtcaccttc gtgaatacca agacctgctc aatgttaaga tggcccttga
     1981 cattgagatt gccacctaca ggaagctgct ggaaggcgag gagagcagga tttctctgcc
     2041 tcttccaaac ttttcctccc tgaacctgag ggaaactaat ctggattcac tccctctggt
     2101 tgatacccac tcaaaaagga cacttctgat taagacggtt gaaactagag atggacaggt
     2161 tatcaacgaa acttctcagc atcacgatga ccttgaataa ggatccaccg gatctagata
     2221 actgatcata atcagccata ccacatt
//
