LOCUS       sequence-84433-e        2231 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 487, Emission: 
            509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2231)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Vimentin-N-18
FEATURES             Location/Qualifiers
     source          1..2231
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1470..1479
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1476..1478,1479..1481,1482..2195)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1521..1542)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1782..1801)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2129..2150
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgcc accatgtcca ccaggtccgt gtcctcgtcc tcctaccgca
       61 ggatgttcgg cggcccgggc accgcgagcc ggccgagctc cagccggagc tacgtgacta
      121 cgtccacccg cacctacagc ctgggcagcg cgctgcgccc cagcaccagc cgcagcctct
      181 acgcctcgtc cccgggcggc gtgtatgcca cgcgctcctc tgccgtgcgc ctgcggagca
      241 gcgtgcccgg ggtgcggctc ctgcaggact cggtggactt ctcgctggcc gacgccatca
      301 acaccgagtt caagaacacc cgcaccaacg agaaggtgga gctgcaggag ctgaatgacc
      361 gcttcgccaa ctacatcgac aaggtgcgct tcctggagca gcagaataag atcctgctgg
      421 ccgagctcga gcagctcaag ggccaaggca agtcgcgcct gggggacctc tacgaggagg
      481 agatgcggga gctgcgccgg caggtggacc agctaaccaa cgacaaagcc cgcgtcgagg
      541 tggagcgcga caacctggcc gaggacatca tgcgcctccg ggagaaattg caggaggaga
      601 tgcttcagag agaggaagcc gaaaacaccc tgcaatcttt cagacaggat gttgacaatg
      661 cgtctctggc acgtcttgac cttgaacgca aagtggaatc tttgcaagaa gagattgcct
      721 ttttgaagaa actccacgaa gaggaaatcc aggagctgca ggctcagatt caggaacagc
      781 atgtccaaat cgatgtggat gtttccaagc ctgacctcac ggctgccctg cgtgacgtac
      841 gtcagcaata tgaaagtgtg gctgccaaga acctgcagga ggcagaagaa tggtacaaat
      901 ccaagtttgc tgacctctct gaggctgcca accggaacaa tgacgccctg cgccaggcaa
      961 agcaggagtc cactgagtac cggagacagg tgcagtccct cacctgtgaa gtggatgccc
     1021 ttaaaggaac caatgagtcc ctggaacgcc agatgcgtga aatggaagag aactttgccg
     1081 ttgaagctgc taactaccaa gacactattg gccgcctgca ggatgagatt cagaatatga
     1141 aggaggaaat ggctcgtcac cttcgtgaat accaagacct gctcaatgtt aagatggccc
     1201 ttgacattga gattgccacc tacaggaagc tgctggaagg cgaggagagc aggatttctc
     1261 tgcctcttcc aaacttttcc tccctgaacc tgagggaaac taatctggat tcactccctc
     1321 tggttgatac ccactcaaaa aggacacttc tgattaagac ggttgaaact agagatggac
     1381 aggttatcaa cgaaacttct cagcatcacg atgaccttga aagcagcggt ggagcaagcg
     1441 cagccagtgg tagcgcggat ccaccggtcg ccaccatggt gagcaagggc gaggagctgt
     1501 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     1561 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     1621 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttg acctacggcg
     1681 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     1741 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     1801 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     1861 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     1921 acaaggtcta tatcaccgcc gacaagcaga agaacggcat caaggtgaac ttcaagaccc
     1981 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     2041 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga
     2101 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     2161 ggatcactct cggcatggac gagctgtaca agtaagcggc cgcgactcta gatcataatc
     2221 agccatacca c
//
