LOCUS       sequence-84435-e        4217 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4217)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Vinculin-N-18
FEATURES             Location/Qualifiers
     source          1..4217
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      3472..3481
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3478..3480,3481..3483,3484..4197)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3523..3544)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3784..3803)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4131..4152
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gactcagatc tcgaggccac catgccagtg tttcatacgc gcacgatcga gagcatcctg
       61 gagccggtgg cacagcagat ttcccacctg gtgataatgc acgaggaggg cgaggtggac
      121 ggcaaagcca ttcctgacct caccgcgccc gtggccgccg tgcaggcggc cgtcagcaac
      181 ctcgtccggg ttggaaaaga gactgttcaa accactgagg atcagatttt gaagagagat
      241 atgccaccag catttattaa ggttgagaat gcttgcacca aacttgtcca ggcagctcag
      301 atgcttcagt cagaccctta ctcagtgcct gctcgtgatt atctaattga tgggtcaagg
      361 ggcatcctct ctggaacatc agacctgctc cttaccttcg atgaggctga ggtccgtaaa
      421 attattagag tttgcaaagg aattttggaa tatcttacag tggcagaggt ggtggagact
      481 atggaagatt tggtcactta cacaaagaat cttgggccag gaatgactaa gatggccaag
      541 atgattgacg agagacagca ggagctcact caccaggagc accgagtgat gttggtgaac
      601 tcgatgaaca ccgtgaaaga gttgctgcca gttctcattt cagctatgaa gatttttgta
      661 acaactaaaa actcaaaaaa ccaaggcata gaggaagcgt taaaaaatcg caattttact
      721 gtagaaaaaa tgagtgctga aattaatgag ataattcgtg tgttacaact cacctcttgg
      781 gatgaagatg cctgggccag caaggacact gaagccatga agagagcatt ggcctccata
      841 gactccaaac tgaaccaggc caaaggttgg ctccgtgacc ctagtgcctc cccaggggat
      901 gctggtgagc aggccatcag acagatttta gatgaagctg gaaaagttgg tgaactctgt
      961 gcaggcaaag aacgcaggga gattctggga acttgcaaaa tgctagggca gatgactgat
     1021 caagtggctg acctccgtgc cagaggacaa ggctcctcac cagtggccat gcagaaagct
     1081 cagcaggtat ctcagggtct ggatgtgctc acagcaaaag tggaaaatgc agctcgcaag
     1141 ctggaagcca tgaccaactc aaagcagagc attgcaaaga agatcgatgc tgctcagaac
     1201 tggcttgcag atccaaatgg tggaccggaa ggagaagagc agattcgagg tgctttggct
     1261 gaagctcgga aaatagcaga attatgtgat gatcctaaag aaagagatga cattctacgt
     1321 tcccttgggg aaatatctgc tctgacttct aaattagcag acctacgaag acaggggaaa
     1381 ggagattctc cagaggctcg ggccttggcc aaacaggtgg ccacggccct gcagaacctg
     1441 cagaccaaaa ccaaccgggc tgtggccaac agcagaccgg ccaaagcagc tgtacacctt
     1501 gagggcaaga ttgagcaagc acagcggtgg attgataatc ccacagtgga tgaccgtgga
     1561 gtcggtcagg ctgccatccg ggggcttgtg gccgaagggc atcgtctggc taatgttatg
     1621 atggggcctt atcggcaaga ccttctcgcc aagtgtgacc gagtggacca gctgacagcc
     1681 cagctggctg acctggctgc cagaggggaa ggggagagtc ctcaggcacg agcacttgca
     1741 tctcagctcc aagactcctt aaaggatcta aaagctcgga tgcaggaggc catgactcag
     1801 gaagtgtcag atgttttcag cgataccaca actcccatca agctgttggc agtggcagcc
     1861 acggcgcctc ctgatgcgcc taacagggaa gaggtatttg atgagagggc agctaacttt
     1921 gaaaaccatt caggaaaact tggtgctacg gccgagaagg cggctgcggt tggtactgct
     1981 aataaatcaa cagtggaagg cattcaggcc tcagtgaaga cggcccgaga actcacaccc
     2041 caggtggtct cggctgctcg tatcttactt aggaaccctg gaaatcaagc tgcttatgaa
     2101 cattttgaga ccatgaagaa ccagtggatc gataatgttg aaaaaatgac agggctggtg
     2161 gacgaagcca ttgataccaa atctctgttg gatgcttcag aagaagcaat taaaaaagac
     2221 ctggacaagt gcaaggtagc tatggccaac attcagcctc agatgctggt tgctggggca
     2281 accagtattg ctcgtcgggc caaccggatt ctgctggtgg ctaagaggga ggtggagaac
     2341 tccgaggacc ccaagttccg tgaggctgtg aaagctgcct ctgatgaatt gagcaaaacc
     2401 atctccccga tggtgatgga tgcaaaagct gtggctggaa acatttccga ccctggactg
     2461 caaaagagct tcctggactc aggatatcgg attctgggag ctgtggccaa ggtcagagaa
     2521 gccttccaac ctcaggagcc tgacttcccg ccgcctccac cagaccttga acaactccga
     2581 ctaacagatg agcttgctcc tcccaaacca cctctgcctg aaggtgaggt ccctccacct
     2641 aggcctccac caccagagga aaaggatgaa gagttccctg agcagaaggc cggggaggtg
     2701 attaaccagc caatgatgat ggctgccaga cagctccatg atgaagctcg caaatggtcc
     2761 agcaagccgg gcatcccagc cgctgaggtg ggtataggtg ttgtagctga ggcagatgcg
     2821 gccgatgctg ctggcttccc tgtcccccct gacatggaag acgattacga acctgagctg
     2881 ctgttaatgc catccaatca gccggtcaac cagcccattc tggccgcggc tcagtccttg
     2941 catcgggaag ctaccaagtg gtctagtaag ggcaatgaca tcattgcagc agccaagcgc
     3001 atggctctgc tgatggctga gatgtctcgg ctggtaagag ggggcagtgg taccaagcgg
     3061 gcactcattc agtgtgccaa ggacatcgcc aaggcctcag atgaggtgac tcggttggcc
     3121 aaggaggttg ccaagcagtg cacagataaa cggattagaa ccaacctctt acaggtatgt
     3181 gagcgaatcc caaccataag cacccagctc aaaatcctgt ccacagtgaa ggccaccatg
     3241 ctgggccgga ccaacatcag tgatgaggag tctgagcagg ccacagagat gctggttcac
     3301 aatgcccaga acctcatgca gtctgtgaag gagactgtgc gggaagctga agctgcttca
     3361 atcaaaattc gaacagatgc tggatttaca ctgcgctggg ttagaaagac tccctggtac
     3421 cagtccggta gcggtggcgg aagcggcggg agcgcagggg atccaccggt cgccaccatg
     3481 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     3541 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     3601 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     3661 gtgaccacct tgacctacgg cgtgcagtgc ttcgcccgct accccgacca catgaagcag
     3721 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     3781 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     3841 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     3901 ctggagtaca actacaacag ccacaaggtc tatatcaccg ccgacaagca gaagaacggc
     3961 atcaaggtga acttcaagac ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     4021 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     4081 ctgagcaccc agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     4141 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaagcg
     4201 gccgcgactc tagatca
//
