LOCUS       sequence-84436-e        4047 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Burnette DT, Manley S, Sengupta P, Sougrat R, Davidson MW, Kachar B,
            Lippincott-Schwartz J
  TITLE     A role for actin arcs in the leading-edge advance of migrating 
            cells.
  JOURNAL   Nat Cell Biol. 2011 Apr;13(4):371-81. doi: 10.1038/ncb2205. Epub 
            2011 Mar 20.
  PUBMED    21423177
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2205"; 
            journalName: "Nat Cell Biol"; date: "2011-04"; volume: "13"; issue: 
            "4"; pages: "371-81"
COMMENT     Sequence Label: mEmerald-Vinculin-N-21
FEATURES             Location/Qualifiers
     source          1..4047
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      3283..3292
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3289..3291,3292..3294,3295..4008)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3334..3355)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3595..3614)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3942..3963
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcac cgccaccatg ccagtgtttc atacgcgcac gatcgagagc
       61 atcctggagc cggtggcaca gcagatctcc cacctggtga taatgcacga ggagggcgag
      121 gtggacggca aagccattcc tgacctcacc gcgcccgtgg ccgccgtgca ggcggccgtc
      181 agcaacctcg tccgggttgg aaaagagact gttcaaacca ctgaggatca gattttgaag
      241 agagatatgc caccagcatt tattaaggtt gagaatgctt gcaccaagct tgtccaggca
      301 gctcagatgc ttcagtcaga cccttactca gtgcctgctc gagattatct aattgatggg
      361 tcaaggggca tcctctctgg aacatcagac ctgctcctta ccttcgatga ggctgaggtc
      421 cgtaaaatta ttagagtttg caaaggaatt ttggaatatc ttacagtggc agaggtggtg
      481 gagactatgg aagatttggt cacttacaca aagaatcttg ggccaggaat gactaagatg
      541 gccaagatga ttgacgagag acagcaggag ctcactcacc aggagcaccg agtgatgttg
      601 gtgaactcga tgaacaccgt gaaagagttg ctgccagttc tcatttcagc tatgaagatt
      661 tttgtaacaa ctaaaaactc aaaaaaccaa ggcatagagg aagctttaaa aaatcgcaat
      721 tttactctag aaaaaatgag tgctgaaatt aatgagataa ttcgtgtgtt acaactcacc
      781 tcttgggatg aagatgcctg ggccagcaag gacactgaag ccatgaagag agcattggcc
      841 tccatagact ccaaactgaa ccaggccaaa ggttggctcc gtgaccctag tgcctcccca
      901 ggggatgctg gtgagcaggc catcagacag atcttagatg aagctggaaa agttggtgaa
      961 ctctgtgcag gcaaagaacg cagggagatt ctgggaactt gcaaaatgct agggcagatg
     1021 actgatcaag tggctgacct ccgtgccaga ggacaaggat cctcaccggt ggccatgcag
     1081 aaagctcagc aggtatctca gggtctggat gtgctcacag caaaagtgga aaatgcagct
     1141 cgcaagctgg aagccatgac caactcaaag cagagcattg caaagaagat cgatgctgct
     1201 cagaactggc ttgcagatcc aaatggtgga ccggaaggag aagagcagat tcgaggtgct
     1261 ttggctgaag ctcggaaaat agcagaatta tgtgatgatc ctaaagaaag agatgacatt
     1321 ctacgttccc ttggggaaat atctgctctg acttctaaat tagcagatct acgaagacag
     1381 gggaaaggag attctccaga ggctcgagcc ttggccaaac aggtggccac ggccctgcag
     1441 aacctgcaga ccaaaaccaa ccgggctgtg gccaacagca gaccggccaa agcagctgta
     1501 caccttgagg gcaagattga gcaagcacag cggtggattg ataatcccac agtggatgac
     1561 cgtggagtcg gtcaggctgc catccggggg cttgtggccg aagggcatcg tctggctaat
     1621 gttatgatgg ggccttatcg gcaagatctt ctcgccaagt gtgaccgagt ggaccagctg
     1681 acagcccagc tggctgacct ggctgccaga ggggaagggg agagtcctca ggcacgagca
     1741 cttgcatctc agctccaaga ctccttaaag gatctaaaag ctcggatgca ggaggccatg
     1801 actcaggaag tgtcagatgt tttcagcgat accacaactc ccatcaagct gttggcagtg
     1861 gcagccacgg cgcctcctga tgcgcctaac agggaagagg tatttgatga gagggcagct
     1921 aactttgaaa accattcagg aaagcttggt gctacggccg agaaggcggc tgcggttggt
     1981 actgctaata aatcaacagt ggaaggcatt caggcctcag tgaagacggc ccgagaactc
     2041 acaccccagg tggtctcggc tgctcgtatc ttacttagga accctggaaa tcaagctgct
     2101 tatgaacatt ttgagaccat gaagaaccag tggatcgata atgttgaaaa aatgacaggg
     2161 ctggtggacg aagccattga taccaaatct ctgttggatg cttcagaaga agcaattaaa
     2221 aaagacctgg acaagtgcaa ggtagctatg gccaacattc agcctcagat gctggttgct
     2281 ggggcaacca gtattgctcg tcgggccaac cggatcctgc tggtggctaa gagggaggtg
     2341 gaaaattccg aggatcccaa gttccgtgag gctgtgaaag ctgcctctga tgaattgagc
     2401 aaaaccatct ccccgatggt gatggatgca aaagctgtgg ctggaaacat ttccgaccct
     2461 ggactgcaaa agagcttcct ggactcagga tatcggatcc tgggagctgt ggccaaggtc
     2521 agagaagcct tccaacctca ggagcctgac ttcccgccgc ctccaccaga ccttgaacaa
     2581 ctccgactaa cagatgagct tgctcctccc aaaccacctc tgcctgaagg tgaggtccct
     2641 ccacctaggc ctccaccacc agaggaaaag gatgaagagt tccctgagca gaaggccggg
     2701 gaggtgatta accagccaat gatgatggct gccagacagc tccatgatga agctcgcaaa
     2761 tggtccagca agggcaatga catcattgca gcagccaagc gcatggctct gctgatggct
     2821 gagatgtctc ggctggtaag agggggcagt ggtaccaagc gggcactcat tcagtgtgcc
     2881 aaggacatcg ccaaggcctc agatgaggtg actcggttgg ccaaggaggt tgccaagcag
     2941 tgcacagata aacggattag aaccaacctc ttacaggtat gtgagcgaat cccaaccata
     3001 agcacccagc tcaaaatcct gtccacagtg aaggccacca tgctgggccg gaccaacatc
     3061 agtgatgagg agtctgagca ggccacagag atgctggttc acaatgccca gaacctcatg
     3121 cagtctgtga aggagactgt gcgggaagct gaagctgctt caatcaaaat tcgaacagat
     3181 gctggattta cactgcgctg ggttagaaag actccctggt accagtccgg tggcagcgga
     3241 attctgcagt cgacggtacc gcgggcccgg gatccaccgg tcgccaccat ggtgagcaag
     3301 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     3361 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     3421 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     3481 ttgacctacg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc
     3541 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     3601 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     3661 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     3721 aactacaaca gccacaaggt ctatatcacc gccgacaagc agaagaacgg catcaaggtg
     3781 aacttcaaga cccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     3841 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     3901 cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     3961 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaagc ggccgcgact
     4021 ctagatcata atcagccata ccacatt
//
