LOCUS       sequence-84437-e        2862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Rizzo MA, Davidson MW, Piston DW
  TITLE     Fluorescent protein tracking and detection: fluorescent protein 
            structure and color variants.
  JOURNAL   Cold Spring Harb Protoc. 2009 Dec;2009(12):pdb.top63. doi: 
            10.1101/pdb.top63.
  PUBMED    20150100
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cold Spring
            Harb Protoc."; date: "2009-12"; volume: "2009(12):pdb.top63. doi"; 
            pages: " 10.1101/pdb.top63"
COMMENT     Sequence Label: mEmerald-Vinexin-C-14
FEATURES             Location/Qualifiers
     source          1..2862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      32..41
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(38..40,41..43,44..754)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(83..104)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(344..363)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     691..712
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agtgacgtca gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct
       61 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
      121 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
      181 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tgacctacgg
      241 cgtgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
      301 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
      361 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
      421 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
      481 ccacaaggtc tatatcaccg ccgacaagca gaagaacggc atcaaggtga acttcaagac
      541 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
      601 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct
      661 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
      721 cgggatcact ctcggcatgg acgagctgta caagtccgga ctcagatctg gtggcagtgg
      781 agggtccgca ggtagccagg gcccaccccg cagcctccgc gctgggctca gcctggacga
      841 cttcatccct ggccacctcc agtcccacat agggtcttcc tcccggggga cacgggtgcc
      901 cgtgatccgg aatggtggct ccaacaccct taatttccag ttccacgacc ccgcgcccag
      961 gactgtgtgc aatgggggct acacaccaag acgagatgct tcccagcacc cggaccctgc
     1021 gtggtatcag acctggccag gccctgggag caagccctct gcaagcacaa agatccctgc
     1081 ctcccagcac acccagaact ggtcagccac gtggaccaag gacagcaagc gtcgggacaa
     1141 gcgctgggtc aagtacgagg gaatcgggcc cgtggacgag agcggcatgc ccattgcccc
     1201 ccgatccagc gttgacagac ccagagactg gtaccggaga atgttccagc agattcaccg
     1261 gaaaatgcca gacttgcagc tggactggac cttcgaggag ccacccagag accccaggca
     1321 tctaggagcc cagcaaagac ctgcccacag gcccggcccg gcaacatctt ccagtggaag
     1381 aagctgggac cactctgaag agttacctag aagcaccttc aactacagac ctggagcatt
     1441 ctccactgtg ctgcagccct caaatcaggt gctcagacgc cgggaaaaag tagacaatgt
     1501 ctggacggaa gagtcctgga accagtttct gcaggaacta gagactgggc agaggcccaa
     1561 gaaaccgctg gtggacgacc ctggtgagaa gccctcccag cccattgagg tgctgctgga
     1621 gagagagctg gccgagctga gcgccgagct ggacaaggac ctgcgggcaa ttgagacccg
     1681 actgccgtcc cccaagagct cgccggcgcc ccgacgggcc ccggagcagc ggcccccggc
     1741 cggcccggcc tcagcctgga gctccagcta cccacatgca ccttacctgg gttccgcccg
     1801 gtccctgagt ccccacaaaa tggctgatgg aggaagcccc ttcctaggtc ggagggactt
     1861 tgtctaccct tcctcaaccc gagaccctag tgcctctaac ggagggggca gcccagccag
     1921 gagggaagag aagaagagaa aggccgccag gctcaagttt gacttccagg cgcagtcccc
     1981 caaggagctg actctgcaga agggtgacat tgtctacatc cacaaggagg tggacaagaa
     2041 ctggctggag ggagagcacc acggccgcct gggcatcttc cctgctaatt atgtggaggt
     2101 gctgcccgca gatgagatcc ctaagcccat caagcccccg acctaccagg tgctggagta
     2161 tggagaggct gtggcccagt acaccttcaa gggggacctg gaggtggagc tgtccttccg
     2221 caagggagag cacatctgcc tgatccgcaa ggtgaacgag aactggtacg agggacgcat
     2281 cacgggcacg gggcgccaag gcatattccc tgccagctac gtgcaggtgt ctcgtgaacc
     2341 ccggctccgg ctctgtgacg acggccccca gctccccacg tctccccgcc tgaccgctgc
     2401 cgcccgctca gcccgtcacc ccagctcccc ctcagccctg cgcagcccag ctgaccccac
     2461 cgacttgggg ggacagacct ccccccgtcg cactggcttc tccttcccca cccaggagcc
     2521 tagaccccag acccagaatc ttggcacccc tggtccagct ctgtcccact ctcgaggtcc
     2581 cagccatccc ctggacctgg ggacctcctc tcctaacacc tctcagatac actggacccc
     2641 gtaccgggcg atgtaccagt acaggcccca gaacgaagac gagctggagc tgcgcgaggg
     2701 ggacagggtg gatgtcatgc agcagtgtga cgatggctgg tttgtgggtg tctcccggag
     2761 gacccagaaa ttcggaacgt tccctggaaa ttacgttgcc ccggtgtgag aattctgcag
     2821 tcgacggtac cgcgggcccg ggatccaccg gatctagata ac
//
