LOCUS       sequence-84438-e        2840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2840)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Vinexin-N-12
FEATURES             Location/Qualifiers
     source          1..2840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2075..2084
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2081..2083,2084..2086,2087..2800)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2126..2147)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2387..2406)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2734..2755
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gctagtgacg tcagatccgc tagcagccac catgcagggc ccaccccgca gcctccgcgc
       61 tgggctcagc ctggacgact tcatccctgg ccacctccag tcccacatag ggtcttcctc
      121 ccgggggaca cgggtgcccg tgatccggaa tggtggctcc aacaccctta atttccagtt
      181 ccacgacccc gcgcccagga ctgtgtgcaa tgggggctac acaccaagac gagatgcttc
      241 ccagcacccg gaccctgcgt ggtatcagac ctggccaggc cctgggagca agccctctgc
      301 aagcacaaag atccctgcct cccagcacac ccagaactgg tcagccacgt ggaccaagga
      361 cagcaagcgt cgggacaagc gctgggtcaa gtacgaggga atcgggcccg tggacgagag
      421 cggcatgccc attgcccccc gatccagcgt tgacagaccc agagactggt accggagaat
      481 gttccagcag attcaccgga aaatgccaga cttgcagctg gactggacct tcgaggagcc
      541 acccagagac cccaggcatc taggagccca gcaaagacct gcccacaggc ccggcccggc
      601 aacatcttcc agtggaagaa gctgggacca ctctgaagag ttacctagaa gcaccttcaa
      661 ctacagacct ggagcattct ccactgtgct gcagccctca aatcaggtgc tcagacgccg
      721 ggaaaaagta gacaatgtct ggacggaaga gtcctggaac cagtttctgc aggaactaga
      781 gactgggcag aggcccaaga aaccgctggt ggacgaccct ggtgagaagc cctcccagcc
      841 cattgaggtg ctgctggaga gagagctggc cgagctgagc gccgagctgg acaaggacct
      901 gcgggcaatt gagacccgac tgccgtcccc caagagctcg ccggcgcccc gacgggcccc
      961 ggagcagcgg cccccggccg gcccggcctc agcctggagc tccagctacc cacatgcacc
     1021 ttacctgggt tccgcccggt ccctgagtcc ccacaaaatg gctgatggag gaagcccctt
     1081 cctaggtcgg agggactttg tctacccttc ctcaacccga gaccctagtg cctctaacgg
     1141 agggggcagc ccagccagga gggaagagaa gaagagaaag gccgccaggc tcaagtttga
     1201 cttccaggcg cagtccccca aggagctgac tctgcagaag ggtgacattg tctacatcca
     1261 caaggaggtg gacaagaact ggctggaggg agagcaccac ggccgcctgg gcatcttccc
     1321 tgctaattat gtggaggtgc tgcccgcaga tgagatccct aagcccatca agcccccgac
     1381 ctaccaggtg ctggagtatg gagaggctgt ggcccagtac accttcaagg gggacctgga
     1441 ggtggagctg tccttccgca agggagagca catctgcctg atccgcaagg tgaacgagaa
     1501 ctggtacgag ggacgcatca cgggcacggg gcgccaaggc atattccctg ccagctacgt
     1561 gcaggtgtct cgtgaacccc ggctccggct ctgtgacgac ggcccccagc tccccacgtc
     1621 tccccgcctg accgctgccg cccgctcagc ccgtcacccc agctccccct cagccctgcg
     1681 cagcccagct gaccccaccg acttgggggg acagacctcc ccccgtcgca ctggcttctc
     1741 cttccccacc caggagccta gaccccagac ccagaatctt ggcacccctg gtccagctct
     1801 gtcccactct cgaggtccca gccatcccct ggacctgggg acctcctctc ctaacacctc
     1861 tcagatacac tggaccccgt accgggcgat gtaccagtac aggccccaga acgaagacga
     1921 gctggagctg cgcgaggggg acagggtgga tgtcatgcag cagtgtgacg atggctggtt
     1981 tgtgggtgtc tcccggagga cccagaaatt cggaacgttc cctggaaatt acgttgcccc
     2041 ggtggcatcc ggcggtagcg gggatccacc ggtcgccacc atggtgagca agggcgagga
     2101 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     2161 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     2221 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttgaccta
     2281 cggcgtgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     2341 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     2401 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     2461 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     2521 cagccacaag gtctatatca ccgccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     2581 gacccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     2641 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccaa
     2701 gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     2761 cgccgggatc actctcggca tggacgagct gtacaagtaa gcggccgcga ctctagatca
     2821 taatcagcca taccacattg
//
