LOCUS       sequence-84447-e        1198 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  full-length native Gal4 with stop in Gateway middle entry clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Fujimoto E, Gaynes B, Brimley CJ, Chien CB, Bonkowsky JL
  TITLE     Gal80 intersectional regulation of cell-type specific expression in 
            vertebrates.
  JOURNAL   Dev Dyn. 2011 Oct;240(10):2324-34. doi: 10.1002/dvdy.22734. Epub 
            2011 Sep 8.
  PUBMED    21905164
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/dvdy.22734"; journalName: "Dev Dyn"; date: "2011-10"; 
            volume: "240"; issue: "10"; pages: "2324-34"
COMMENT     Sequence Label: pME-Gal4
FEATURES             Location/Qualifiers
     source          1..1198
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    14..113
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             119..559
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
ORIGIN
        1 taagctcggg ccccaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca
       61 aattgatgag caatgctttt ttataatgcc aactttgtac aaaaaagcag gctagaccat
      121 gaagctactg tcttctatcg aacaagcatg cgatatttgc cgacttaaaa agctcaagtg
      181 ctccaaagaa aaaccgaagt gcgccaagtg tctgaagaac aactgggagt gtcgctactc
      241 tcccaaaacc aaaaggtctc cgctgactag ggcacatctg acagaagtgg aatcaaggct
      301 agaaagactg gaacagctat ttctactgat ttttcctcga gaagaccttg acatgatttt
      361 gaaaatggat tctttacagg atataaaagc attgttaaca ggattatttg tacaagataa
      421 tgtgaataaa gatgccgtca cagatagatt ggcttcagtg gagactgata tgcctctaac
      481 attgagacag catagaataa gtgcgacatc atcatcggaa gagagtagta acaaaggtca
      541 aagacagttg actgtatcga ttgactcggc agctcatcat gataactcca caattccgtt
      601 ggattttatg cccagggatg ctcttcatgg atttgattgg tctgaagagg atgacatgtc
      661 ggatggcttg cccttcctga aaacggaccc caacaataat gggttctttg gcgacggttc
      721 tctcttatgt attcttcgat ctattggctt taaaccggaa aattacacga actctaacgt
      781 taacaggctc ccgaccatga ttacggatag atacacgttg gcttctagat ccacaacatc
      841 ccgtttactt caaagttatc tcaataattt tcacccctac tgccctatcg tgcactcacc
      901 gacgctaatg atgttgtata ataaccagat tgaaatcgcg tcgaaggatc aatggcaaat
      961 cctttttaac tgcatattag ccattggagc ctggtgtata gagggggaat ctactgatat
     1021 agatgttttt tactatcaaa atgctaaatc tcatttgacg agcaaggtct tcgagtcagg
     1081 ttccataatt ttggtgacag ccctacatct tctgtcgcga tatacacagt ggaggcagaa
     1141 aacaaatact agctataatt ttcacagctt ttccataaga atggccatat cattgggc
//
