LOCUS       sequence-84463-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Targeting vector for Human MBD3 conditional knockout. Also creates 
            hypomorphic alleles..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Rais Y, Zviran A, Geula S, Gafni O, Chomsky E, Viukov S, Mansour AA,
            Caspi I, Krupalnik V, Zerbib M, Maza I, Mor N, Baran D, Weinberger 
            L, Jaitin DA, Lara-Astiaso D, Blecher-Gonen R, Shipony Z, Mukamel Z,
            Hagai T, Gilad S, Amann-Zalcenstein D, Tanay A, Amit I, Novershtern 
            N, Hanna JH
  TITLE     Deterministic direct reprogramming of somatic cells to pluripotency.
  JOURNAL   Nature. 2013 Oct 3;502(7469):65-70. doi: 10.1038/nature12587. Epub 
            2013 Sep 18.
  PUBMED    24048479
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature12587"; journalName: "Nature"; date: "2013-10-3- 3"; 
            volume: "502"; issue: "7469"; pages: "65-70"
COMMENT     Sequence Label: pNTK human MBD3 PGK-Neo-pA flox
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(118..135)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    124..348
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(391..424)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
       61 atcgccttct tgacgagttc ttctgagggg atcaattctc tagagctcgc tgatcagcct
      121 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
      181 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
      241 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
      301 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
      361 aaagaaccag ctgggggcgc gcccctcgag gaagttccta tactttctag agaataggaa
      421 cttcggtacc ctagaaagct ttgggatgaa ggtctggggt cacgccaggg ttggggtctg
      481 gggctggggc cagggcctgc ctgggtttgg ggtcagggcc gtgttggggt ctggggctgg
      541 ggtcgcgacc tgaagctggg gctgggactg ggtttgggtc tgcggccgag gcctgggttt
      601 ggggtctggg gtccgcccgg ggcgccgacc gtcccgcctc cccgcagccc gagcgggaag
      661 aagttccgca gcatgccgca gctggcgcgc tacctgggcg gttccatgga cctgagcacc
      721 ttcgacttcc gcacgggcaa gatgctgatg agcaagatga acaagagccg ccagcgcgtg
      781 cgctacgact cctccaacca
//
