LOCUS       sequence-84481-e        9253 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Target INCENP to centromeres via CENP-B DNA binding domain (DBD).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9253)
  AUTHORS   Wang E, Ballister ER, Lampson MA
  TITLE     Aurora B dynamics at centromeres create a diffusion-based 
            phosphorylation gradient.
  JOURNAL   J Cell Biol. 2011 Aug 22;194(4):539-49. doi: 10.1083/jcb.201103044. 
            Epub 2011 Aug 15.
  PUBMED    21844210
REFERENCE   2  (bases 1 to 9253)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201103044"; journalName: "J Cell Biol"; date: 
            "2011-08-22- 22"; volume: "194"; issue: "4"; pages: "539-49"
COMMENT     Sequence Label: Cenp-B DBD INCENP mCherry
FEATURES             Location/Qualifiers
     source          1..9253
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      903..912
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4083..4793
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4228..4246)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4496..4515
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(4809..4827)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4810..4827)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4847..4864)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4853..5077
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5123..5551
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5210..5229)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5420..5441
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5560..5580)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5565..5894
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5745..5880
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5807..5826
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5961..6755
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6015..6034)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6625..6644
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6929..7050
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6966..6985)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7020..7039
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7099..7115)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7099..7115)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7112..7134)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7123..7139
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7147..7153,7154..7171,7172..7177))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7192..7213
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7330..7347)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7501..8086)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7581..7600)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8257..9048,9049..9117))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8880..8899
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9118..9222)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggcgcg
      901 ccgccaccat gggccccaag aggcgacagc tgacgttccg ggagaagtca cggatcatcc
      961 aggaggtgga ggagaatccg gacctgcgca agggcgagat cgcgcggcgc ttcaacatcc
     1021 cgccgtccac gctgagcacg atcctgaaga acaagcgcgc catcctggcg tcggagcgca
     1081 agtacggggt ggcctccacc tgccgcaaga ccaacaagct gtctccctac gacaagctcg
     1141 agggcttgct catcgcctgg ttccagcaga tccgcgccgc cggcctgccg gtcaagggca
     1201 tcatcctcaa ggagaaggcg ctgcgcatag ccgaggagct gggcatggac gacttcaccg
     1261 cctccaacgg ctggctggac cgcttccgcc ggcgccacgg cgtggtgtcc tgcagcggcg
     1321 tggcccgcgc ccgcgcgcga aacgctgccc cccgcacccc ggcggcgcct gccagtccgg
     1381 ccgcggtgcc ctcggagggc agtggcgggc tagcagggag cactgaattc agcaaagagc
     1441 cagagctgat gcccaaaaca ccttctcaga agaaccgacg gaagaagaga cggatttctt
     1501 atgttcagga tgaaaacaga gatcccatca ggagaaggtt atcccgcaga aagtctcgga
     1561 gcagccagct gagctcccga cgcctccgca gcaaggacag tgtagagaag ctggctacag
     1621 tggtcgggga gaacggctcc gttctgcggc gtgtgacccg tgctgcggct gcagctgccg
     1681 cggctaccat ggcattggct gcaccttctt cacccacccc tgagtctccc acgatgctga
     1741 ctaagaagcc cgaggataac cacacccagt gccagctggt gcctgtggtg gagatcggca
     1801 tcagtgagcg ccagaatgct gagcagcatg tcacccagct catgtccacc gagcctctgc
     1861 cccgcactct gtccccgact ccagcttcag ccacagctcc aacctcccag ggcatcccga
     1921 catcagatga ggaatcaaca cctaagaagt cgaaggccag gatactggag tccatcacag
     1981 tgagctccct gatggctaca ccccaggacc ccaagggtca aggggtcggg acggggcggt
     2041 ctgcgtctaa gctcaggatt gcgcaggtct cccctggccc acgggactcg ccagcctttc
     2101 cagattctcc atggcgggag cgggtgctgg ctcccatcct gccggataac ttctccacgc
     2161 ccacgggctc tcgcacggac tctcaatcgg tgcggcacag cccgatcgcc ccgtcttccc
     2221 cgagtccccg agtcttagcc cagaagtact ctctggtggc caaacaggaa agtgttgtcc
     2281 gcagggcgag cagaaggctt gccaagaaga ctgccgaaga gccagctgcc tctggccgca
     2341 tcatctgtca cagttacctg gagaggctcc tgaatgttga ggtgccccag aaagttggtt
     2401 ctgagcagaa ggaacccccc gaggaggctg agcctgtggc ggcagctgag ccagaggtcc
     2461 ctgagaacaa cggaaataac tcgtggcccc acaatgacac agagatcgcc aacagcacac
     2521 ccaacccgaa gcctgcagcc agcagcccgg aaacaccctc tgcagggcag caagaggcca
     2581 agacggacca agcagatgga cccagagagc caccgcagag tgccaggagg aagcgcagct
     2641 acaagcaggc cgtgagtgag ctggacgagg agcagcacct ggaggatgag gagctgcagc
     2701 cccccaggag caagacccct tcctcaccct gcccagccag caaggtggta cggcccctcc
     2761 ggacctttct gcacacagtg cagaggaacc agatgctcac gaccccgacc tcagccccac
     2821 gcagcgtcat gaagtccttt attaagcgca acactcccct gcgcatggac cccaagtgca
     2881 gcttcgtcga gaaggagcgg cagcgcctgg agaatctgcg gcggaaggag gaggccgagc
     2941 agctgcgcag gcagaaggtg gaggaggaca agcggcggcg gctggaggag gtgaagctga
     3001 agcgtgagga acgcctccgc aaggtgctgc aggcccgcga gcgggtggag cagatgaagg
     3061 aggagaagaa gaagcagatt gagcagaagt ttgctcagat cgacgagaag actgagaagg
     3121 ccaaggagga gcggctggca gaggagaagg ccaagaaaaa ggcggcggcc aagaagatgg
     3181 aggaggtgga agcacgcagg aagcaggaag aggatgcacg taggctcagg tggctgcagc
     3241 aggaggagga agagcggcgg caccaagagc tgctgcagaa gaagaaggaa gaggagcagg
     3301 agcggctgcg gaaggcggcc gaggctaagc ggctggcaga gcagcgggag caggagcggc
     3361 gggagcagga gcggcgcgag caggagcggc gcgagcagga gcggcgggag caggagcggc
     3421 gcgagcagga gcgacagctg gcagagcagg agcgtcggcg ggagcaggag cggctccagg
     3481 ccgagaggga gctgcaggag cgggagaagg ccctgcggct gcagaaggag cagctgcaga
     3541 gggaactgga ggagaagaag aagaaggaag agcagcagcg tctggctgag cggcagctgc
     3601 aggaggagca agagaagaaa gccaaggagg cagcaggggc cagcaaggcc ctgaatgtga
     3661 ctgtggacgt gcagtctcca gcttgtacct catatcagat gactccgcaa gggcacaggg
     3721 cccctcccaa gatcaaccca gataactacg ggatggatct gaatagcgac gactccaccg
     3781 atgatgaggc ccatccccgg aagcccatcc ccacctgggc ccgaggcacc ccgctcagcc
     3841 aggctatcat tcaccagtac taccacccac cgaaccttct ggagctcttt ggaaccattc
     3901 tcccactgga cttggaggat atcttcaaga agagcaagcc ccgctatcac aagcgcacca
     3961 gctctgctgt ctggaactca ccgcccctgc agggcgccag ggtccccagc agcctggcct
     4021 acagcctgaa gaagcacaag cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc
     4081 caatggtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
     4141 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
     4201 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
     4261 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
     4321 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
     4381 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
     4441 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
     4501 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
     4561 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
     4621 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
     4681 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     4741 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag taatctagag
     4801 ggccctattc tatagtgtca cctaaatgct agagctcgct gatcagcctc gactgtgcct
     4861 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     4921 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     4981 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     5041 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     5101 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     5161 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     5221 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     5281 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     5341 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     5401 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     5461 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     5521 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     5581 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     5641 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     5701 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     5761 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     5821 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     5881 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga
     5941 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     6001 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     6061 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     6121 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     6181 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     6241 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     6301 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     6361 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     6421 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     6481 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     6541 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     6601 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     6661 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     6721 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
     6781 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     6841 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     6901 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     6961 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     7021 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     7081 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     7141 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     7201 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7261 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     7321 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     7381 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     7441 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     7501 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     7561 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     7621 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     7681 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     7741 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     7801 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     7861 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     7921 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     7981 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggttt
     8041 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     8101 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     8161 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     8221 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     8281 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     8341 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     8401 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     8461 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     8521 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     8581 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     8641 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     8701 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     8761 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     8821 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     8881 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     8941 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     9001 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     9061 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     9121 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     9181 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     9241 gccacctgac gtc
//
