LOCUS       sequence-84485-e        9265 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Target INCENP (with TSS mutated to AAA) to centromeres via CENP-B 
            DNA binding domain (DBD).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9265)
  AUTHORS   Wang E, Ballister ER, Lampson MA
  TITLE     Aurora B dynamics at centromeres create a diffusion-based 
            phosphorylation gradient.
  JOURNAL   J Cell Biol. 2011 Aug 22;194(4):539-49. doi: 10.1083/jcb.201103044. 
            Epub 2011 Aug 15.
  PUBMED    21844210
REFERENCE   2  (bases 1 to 9265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201103044"; journalName: "J Cell Biol"; date: 
            "2011-08-22- 22"; volume: "194"; issue: "4"; pages: "539-49"
COMMENT     Sequence Label: Cenp-B DBD INCENP TSS/AAA mCherry
FEATURES             Location/Qualifiers
     source          1..9265
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      903..912
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4083..4793
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4228..4246)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4496..4515
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(4821..4839)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4822..4839)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4859..4876)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4865..5089
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5135..5563
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5222..5241)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5432..5453
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5572..5592)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5577..5906
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5757..5892
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5819..5838
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5973..6767
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6027..6046)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6637..6656
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6941..7062
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6978..6997)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7032..7051
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7111..7127)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7111..7127)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7124..7146)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7135..7151
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7159..7165,7166..7183,7184..7189))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7204..7225
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7342..7359)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7513..8098)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7593..7612)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8269..9060,9061..9129))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8892..8911
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9130..9234)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggcgcg
      901 ccgccaccat gggccccaag aggcgacagc tgacgttccg ggagaagtca cggatcatcc
      961 aggaggtgga ggagaatccg gacctgcgca agggcgagat cgcgcggcgc ttcaacatcc
     1021 cgccgtccac gctgagcacg atcctgaaga acaagcgcgc catcctggcg tcggagcgca
     1081 agtacggggt ggcctccacc tgccgcaaga ccaacaagct gtctccctac gacaagctcg
     1141 agggcttgct catcgcctgg ttccagcaga tccgcgccgc cggcctgccg gtcaagggca
     1201 tcatcctcaa ggagaaggcg ctgcgcatag ccgaggagct gggcatggac gacttcaccg
     1261 cctccaacgg ctggctggac cgcttccgcc ggcgccacgg cgtggtgtcc tgcagcggcg
     1321 tggcccgcgc ccgcgcgcga aacgctgccc cccgcacccc ggcggcgcct gccagtccgg
     1381 ccgcggtgcc ctcggagggc agtggcgggc tagcagggag cactgaattc agcaaagagc
     1441 cagagctgat gcccaaaaca ccttctcaga agaaccgacg gaagaagaga cggatttctt
     1501 atgttcagga tgaaaacaga gatcccatca ggagaaggtt atcccgcaga aagtctcgga
     1561 gcagccagct gagctcccga cgcctccgca gcaaggacag tgtagagaag ctggctacag
     1621 tggtcgggga gaacggctcc gttctgcggc gtgtgacccg tgctgcggct gcagctgccg
     1681 cggctaccat ggcattggct gcaccttctt cacccacccc tgagtctccc acgatgctga
     1741 ctaagaagcc cgaggataac cacacccagt gccagctggt gcctgtggtg gagatcggca
     1801 tcagtgagcg ccagaatgct gagcagcatg tcacccagct catgtccacc gagcctctgc
     1861 cccgcactct gtccccgact ccagcttcag ccacagctcc aacctcccag ggcatcccga
     1921 catcagatga ggaatcaaca cctaagaagt cgaaggccag gatactggag tccatcacag
     1981 tgagctccct gatggctaca ccccaggacc ccaagggtca aggggtcggg acggggcggt
     2041 ctgcgtctaa gctcaggatt gcgcaggtct cccctggccc acgggactcg ccagcctttc
     2101 cagattctcc atggcgggag cgggtgctgg ctcccatcct gccggataac ttctccacgc
     2161 ccacgggctc tcgcacggac tctcaatcgg tgcggcacag cccgatcgcc ccgtcttccc
     2221 cgagtccccg agtcttagcc cagaagtact ctctggtggc caaacaggaa agtgttgtcc
     2281 gcagggcgag cagaaggctt gccaagaaga ctgccgaaga gccagctgcc tctggccgca
     2341 tcatctgtca cagttacctg gagaggctcc tgaatgttga ggtgccccag aaagttggtt
     2401 ctgagcagaa ggaacccccc gaggaggctg agcctgtggc ggcagctgag ccagaggtcc
     2461 ctgagaacaa cggaaataac tcgtggcccc acaatgacac agagatcgcc aacagcacac
     2521 ccaacccgaa gcctgcagcc agcagcccgg aaacaccctc tgcagggcag caagaggcca
     2581 agacggacca agcagatgga cccagagagc caccgcagag tgccaggagg aagcgcagct
     2641 acaagcaggc cgtgagtgag ctggacgagg agcagcacct ggaggatgag gagctgcagc
     2701 cccccaggag caagacccct tcctcaccct gcccagccag caaggtggta cggcccctcc
     2761 ggacctttct gcacacagtg cagaggaacc agatgctcac gaccccgacc tcagccccac
     2821 gcagcgtcat gaagtccttt attaagcgca acactcccct gcgcatggac cccaagtgca
     2881 gcttcgtcga gaaggagcgg cagcgcctgg agaatctgcg gcggaaggag gaggccgagc
     2941 agctgcgcag gcagaaggtg gaggaggaca agcggcggcg gctggaggag gtgaagctga
     3001 agcgtgagga acgcctccgc aaggtgctgc aggcccgcga gcgggtggag cagatgaagg
     3061 aggagaagaa gaagcagatt gagcagaagt ttgctcagat cgacgagaag actgagaagg
     3121 ccaaggagga gcggctggca gaggagaagg ccaagaaaaa ggcggcggcc aagaagatgg
     3181 aggaggtgga agcacgcagg aagcaggaag aggatgcacg taggctcagg tggctgcagc
     3241 aggaggagga agagcggcgg caccaagagc tgctgcagaa gaagaaggaa gaggagcagg
     3301 agcggctgcg gaaggcggcc gaggctaagc ggctggcaga gcagcgggag caggagcggc
     3361 gggagcagga gcggcgcgag caggagcggc gcgagcagga gcggcgggag caggagcggc
     3421 gcgagcagga gcgacagctg gcagagcagg agcgtcggcg ggagcaggag cggctccagg
     3481 ccgagaggga gctgcaggag cgggagaagg ccctgcggct gcagaaggag cagctgcaga
     3541 gggaactgga ggagaagaag aagaaggaag agcagcagcg tctggctgag cggcagctgc
     3601 aggaggagca agagaagaaa gccaaggagg cagcaggggc cagcaaggcc ctgaatgtga
     3661 ctgtggacgt gcagtctcca gcttgtacct catatcaagt gactccgcaa gggcacaggg
     3721 cccctcccaa gatcaaccca gataactacg ggatggatct gaatagcgac gactccaccg
     3781 atgatgaggc ccatccccgg aagcccatcc ccacctgggc ccgaggcacc ccgctcagcc
     3841 aggctatcat tcaccagtac taccacccac cgaaccttct ggagctcttt ggaaccattc
     3901 tcccactgga cttggaggat atcttcaaga agagcaagcc ccgctatcac aagcgcgccg
     3961 ccgctgctgt ctggaactca ccgcccctgc agggcgccag ggtccccagc agcctggcct
     4021 acagcctgaa gaagcacaag cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc
     4081 caatggtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
     4141 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
     4201 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
     4261 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
     4321 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
     4381 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
     4441 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
     4501 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
     4561 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
     4621 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
     4681 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     4741 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag taaagcggcc
     4801 gcgactctag agggccctat tctatagtgt cacctaaatg ctagagctcg ctgatcagcc
     4861 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     4921 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     4981 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     5041 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     5101 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     5161 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     5221 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     5281 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     5341 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     5401 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     5461 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     5521 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
     5581 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     5641 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     5701 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     5761 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     5821 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     5881 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
     5941 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     6001 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     6061 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     6121 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     6181 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     6241 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     6301 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     6361 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     6421 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     6481 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     6541 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     6601 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     6661 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     6721 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     6781 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     6841 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     6901 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     6961 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     7021 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     7081 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     7141 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     7201 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     7261 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     7321 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7381 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7441 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7501 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7561 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7621 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7681 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7741 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7801 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     7861 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     7921 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     7981 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8041 tggtagcggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     8101 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     8161 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     8221 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     8281 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     8341 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     8401 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     8461 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     8521 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     8581 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     8641 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     8701 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     8761 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     8821 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     8881 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     8941 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     9001 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     9061 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     9121 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     9181 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     9241 tccccgaaaa gtgccacctg acgtc
//
