LOCUS       sequence-84488-e       10549 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Target INCENP to centromeres via full length CENP-B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10549)
  AUTHORS   Wang E, Ballister ER, Lampson MA
  TITLE     Aurora B dynamics at centromeres create a diffusion-based 
            phosphorylation gradient.
  JOURNAL   J Cell Biol. 2011 Aug 22;194(4):539-49. doi: 10.1083/jcb.201103044. 
            Epub 2011 Aug 15.
  PUBMED    21844210
REFERENCE   2  (bases 1 to 10549)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201103044"; journalName: "J Cell Biol"; date: 
            "2011-08-22- 22"; volume: "194"; issue: "4"; pages: "539-49"
COMMENT     Sequence Label: Cenp-B INCENP mCherry
FEATURES             Location/Qualifiers
     source          1..10549
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      903..912
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5379..6089
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5524..5542)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5792..5811
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(6105..6123)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6106..6123)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6143..6160)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6149..6373
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6419..6847
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6506..6525)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6716..6737
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6856..6876)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6861..7190
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7041..7176
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7103..7122
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7257..8051
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7311..7330)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7921..7940
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8225..8346
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8262..8281)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8316..8335
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8395..8411)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8395..8411)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8408..8430)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8419..8435
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8443..8449,8450..8467,8468..8473))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8488..8509
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8626..8643)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8797..9382)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8877..8896)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9553..10344,10345..10413))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10176..10195
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10414..10518)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggcgcg
      901 ccgccaccat gggccccaag aggcgacagc tgacgttccg ggagaagtca cggatcatcc
      961 aggaggtgga ggagaatccg gacctgcgca agggcgagat cgcgcggcgc ttcaacatcc
     1021 cgccgtccac gctgagcacg atcctgaaga acaagcgcgc catcctggcg tcggagcgca
     1081 agtacggggt ggcctccacc tgccgcaaga ccaacaagct gtctccctac gacaagctcg
     1141 agggcttgct catcgcctgg ttccagcaga tccgcgccgc cggcctgccg gtcaagggca
     1201 tcatcctcaa ggagaaggcg ctgcgcatag ccgaggagct gggcatggac gacttcaccg
     1261 cctccaacgg ctggctggac cgcttccgcc ggcgccacgg cgtggtgtcc tgcagcggcg
     1321 tggcccgcgc ccgcgcgcga aacgctgccc cccgcacccc ggcggcgcct gccagtccgg
     1381 ccgcggtgcc ctcggagggc agtggcggga gcactactgg ttggcgcgct cgggaggagc
     1441 agccgccgtc ggtggccgag ggctacgcct cgcaggacgt gttcagcgcc accgagacca
     1501 gtctatggta cgacttcctg cccgaccagg ccgcggggct gtgcggaggc gacggacggc
     1561 cgcgtcaagc cacccagcgc ctgagcgtcc tgctatgcgc caatgccgac ggcagcgaga
     1621 agctgccccc gctggtggcc ggcaagtcgg ccaagccccg cgcaggccaa gccggcctgc
     1681 cctgcgacta caccgccaac tccaagggtg gtgtcaccac ccaggccctg gccaagtact
     1741 tgaaggcctt ggacacccga atggctgcag agtctcgccg ggtcctgctg ttggccggcc
     1801 gcttggctgc ccagtccttg gacacctcgg gcctgcggca tgtgcagctg gccttcttcc
     1861 ctcccggcac cgtgcatccg ctggagaggg gagtggtcca gcaggtgaag ggccactacc
     1921 gccaggccat gctgctcaag gccatggccg cgctagaggg ccaggatccc tcaggcctgc
     1981 agctgggtct cacggaggcc ctgcactttg tggctgccgc ctggcaggca gtggagcctt
     2041 cggacatagc cgcctgcttt cgtgaggctg gctttggggg tggccctaat gccaccatca
     2101 ccacttccct caagagtgag ggagaggaag aggaggagga ggaggaagaa gaggaggagg
     2161 aagagggtga aggagaggaa gaggaggagg aaggggagga ggaggaggag gaaggggggg
     2221 aaggagagga attgggggag gaagaggagg tggaggagga gggtgatgtt gatagtgatg
     2281 aagaagagga ggaagatgag gagagctcct cggagggctt ggaggctgag gactgggccc
     2341 agggagtagt ggaggccggt ggcagcttcg gggcttatgg tgcccaggag gaagcccagt
     2401 gccctactct gcatttcctg gaaggtgggg aggactctga ttcagacagt gaggaagagg
     2461 acgatgagga agaggatgat gaagatgaag acgacgatga tgatgaggag gatggtgatg
     2521 aggtgcctgt acccagcttt ggggaggcca tggcttactt tgccatggtc aagaggtacc
     2581 tgacctcctt ccccattgat gaccgcgtgc agagccacat cctccacttg gaacacgatc
     2641 tggttcatgt gaccaggaag aaccacgcca ggcaggcggg agttcgaggt cttggacatc
     2701 aaagcgggct agcagggagc actgaattca gcaaagagcc agagctgatg cccaaaacac
     2761 cttctcagaa gaaccgacgg aagaagagac ggatttctta tgttcaggat gaaaacagag
     2821 atcccatcag gagaaggtta tcccgcagaa agtctcggag cagccagcag agcacccgac
     2881 gcctccgcag caaggacagt gtagagaagc tggctacagt ggtcggggag aacggctccg
     2941 ttctgcggcg tgtgacccgt gctgcggctg cagctgccgc ggctaccatg gcattggctg
     3001 caccttcttc acccacccct gagtctccca cgatgctgac taagaagccc gaggataacc
     3061 acacccagtg ccagctggtg cctgtggtgg agatcggcat cagtgagcgc cagaatgctg
     3121 agcagcatgt cacccagctc atgtccaccg agcctctgcc ccgcactctg tccccgactc
     3181 cagcttcagc cacagctcca acctcccagg gcatcccgac atcagatgag gaatcaacac
     3241 ctaagaagtc gaaggccagg atactggagt ccatcacagt gagctccctg atggctacac
     3301 cccaggaccc caagggtcaa ggggtcggga cggggcggtc tgcgtctaag ctcaggattg
     3361 cgcaggtctc ccctggccca cgggactcgc cagcctttcc agattctcca tggcgggagc
     3421 gggtgctggc tcccatcctg ccggataact tctccacgcc cacgggctct cgcacggact
     3481 ctcaatcggt gcggcacagc ccgatcgccc cgtcttcccc gagtccccga gtcttagccc
     3541 agaagtactc tctggtggcc aaacaggaaa gtgttgtccg cagggcgagc agaaggcttg
     3601 ccaagaagac tgccgaagag ccagctgcct ctggccgcat catctgtcac agttacctgg
     3661 agaggctcct gaatgttgag gtgccccaga aagttggttc tgagcagaag gaaccccccg
     3721 aggaggctga gcctgtggcg gcagctgagc cagaggtccc tgagaacaac ggaaataact
     3781 cgtggcccca caatgacaca gagatcgcca acagcacacc caacccgaag cctgcagcca
     3841 gcagcccgga aacaccctct gcagggcagc aagaggccaa gacggaccaa gcagatggac
     3901 ccagagagcc accgcagagt gccaggagga agcgcagcta caagcaggcc gtgagtgagc
     3961 tggacgagga gcagcacctg gaggatgagg agctgcagcc ccccaggagc aagacccctt
     4021 cctcaccctg cccagccagc aaggtggtac ggcccctccg gacctttctg cacacagtgc
     4081 agaggaacca gatgctcacg accccgacct cagccccacg cagcgtcatg aagtccttta
     4141 ttaagcgcaa cactcccctg cgcatggacc ccaagtgcag cttcgtcgag aaggagcggc
     4201 agcgcctgga gaatctgcgg cggaaggagg aggccgagca gctgcgcagg cagaaggtgg
     4261 aggaggacaa gcggcggcgg ctggaggagg tgaagctgaa gcgtgaggaa cgcctccgca
     4321 aggtgctgca ggcccgcgag cgggtggagc agatgaagga ggagaagaag aagcagattg
     4381 agcagaagtt tgctcagatc gacgagaaga ctgagaaggc caaggaggag cggctggcag
     4441 aggagaaggc caagaaaaag gcggcggcca agaagatgga ggaggtggaa gcacgcagga
     4501 agcaggaaga ggatgcacgt aggctcaggt ggctgcagca ggaggaggaa gagcggcggc
     4561 accaagagct gctgcagaag aagaaggaag aggagcagga gcggctgcgg aaggcggccg
     4621 aggctaagcg gctggcagag cagcgggagc aggagcggcg ggagcaggag cggcgcgagc
     4681 aggagcggcg cgagcaggag cggcgggagc aggagcggcg cgagcaggag cgacagctgg
     4741 cagagcagga gcgtcggcgg gagcaggagc ggctccaggc cgagagggag ctgcaggagc
     4801 gggagaaggc cctgcggctg cagaaggagc agctgcagag ggaactggag gagaagaaga
     4861 aggaagagca gcagcgtctg gctgagcggc agctgcagga ggagcaagag aagaaagcca
     4921 aggaggcagc aggggccagc aaggccctga atgtgactgt ggacgtgcag tctccagctt
     4981 gtacctcata tcagatgact ccgcaagggc acagggcccc tcccaagatc aacccagata
     5041 actacgggat ggatctgaat agcgacgact ccaccgatga tgaggcccat ccccggaagc
     5101 ccatccccac ctgggcccga ggcaccccgc tcagccaggc tatcattcac cagtactacc
     5161 acccaccgaa ccttctggag ctctttggaa ccattctccc actggacttg gaggatatct
     5221 tcaagaagag caagccccgc tatcacaagc gcaccagctc tgctgtctgg aactcaccgc
     5281 ccctgcaggg cgccagggtc cccagcagcc tggcctacag cctgaagaag cacaagcttc
     5341 gaattctgca gtcgacggta ccgcgggccc gggatccaat ggtgagcaag ggcgaggagg
     5401 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
     5461 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     5521 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
     5581 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
     5641 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     5701 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
     5761 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
     5821 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
     5881 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
     5941 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct
     6001 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
     6061 ccggcggcat ggacgagctg tacaagtaat ctagagggcc ctattctata gtgtcaccta
     6121 aatgctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     6181 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     6241 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     6301 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     6361 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     6421 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     6481 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     6541 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     6601 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     6661 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     6721 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     6781 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     6841 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     6901 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     6961 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     7021 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     7081 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     7141 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     7201 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga
     7261 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     7321 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     7381 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     7441 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     7501 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     7561 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     7621 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     7681 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     7741 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     7801 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     7861 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     7921 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     7981 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     8041 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     8101 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     8161 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     8221 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     8281 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     8341 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     8401 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     8461 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     8521 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     8581 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     8641 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     8701 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     8761 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     8821 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     8881 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     8941 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     9001 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     9061 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     9121 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     9181 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     9241 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     9301 ttgatccggc aaacaaacca ccgctggtag cggttttttt gtttgcaagc agcagattac
     9361 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     9421 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     9481 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     9541 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     9601 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     9661 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     9721 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     9781 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     9841 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     9901 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     9961 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
    10021 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    10081 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    10141 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    10201 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    10261 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    10321 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    10381 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    10441 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    10501 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc
//
