LOCUS       sequence-84491-e        9871 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Target INCENP to lac operator sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9871)
  AUTHORS   Wang E, Ballister ER, Lampson MA
  TITLE     Aurora B dynamics at centromeres create a diffusion-based 
            phosphorylation gradient.
  JOURNAL   J Cell Biol. 2011 Aug 22;194(4):539-49. doi: 10.1083/jcb.201103044. 
            Epub 2011 Aug 15.
  PUBMED    21844210
REFERENCE   2  (bases 1 to 9871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201103044"; journalName: "J Cell Biol"; date: 
            "2011-08-22- 22"; volume: "194"; issue: "4"; pages: "539-49"
COMMENT     Sequence Label: LacI INCENP
FEATURES             Location/Qualifiers
     source          1..9871
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      903..912
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(931..950)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             1992..2012
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4689..5399
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4834..4852)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5102..5121
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(5427..5445)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5428..5445)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5465..5482)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5471..5695
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5741..6169
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5828..5847)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6038..6059
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6178..6198)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6183..6512
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6363..6498
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6425..6444
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6579..7373
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6633..6652)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7243..7262
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7547..7668
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7584..7603)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7638..7657
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7717..7733)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7717..7733)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7730..7752)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7741..7757
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7765..7771,7772..7789,7790..7795))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7810..7831
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7948..7965)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8119..8704)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8199..8218)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8875..9666,9667..9735))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9498..9517
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9736..9840)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggcgcg
      901 ccgccaccat ggtgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt
      961 atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa
     1021 aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg
     1081 cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt
     1141 cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt
     1201 cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc
     1261 aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg
     1321 aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca
     1381 acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat
     1441 tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc
     1501 gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg
     1561 aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca
     1621 tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca
     1681 ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg
     1741 aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg
     1801 ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc
     1861 agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg
     1921 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgaagca
     1981 gcctgaggcc tcctaagaag aagaggaagg ttgggctagc agggagcact gaattcagca
     2041 aagagccaga gctgatgccc aaaacacctt ctcagaagaa ccgacggaag aagagacgga
     2101 tttcttatgt tcaggatgaa aacagagatc ccatcaggag aaggttatcc cgcagaaagt
     2161 ctcggagcag ccagctgagc tcccgacgcc tccgcagcaa ggacagtgta gagaagctgg
     2221 ctacagtggt cggggagaac ggctccgttc tgcggcgtgt gacccgtgct gcggctgcag
     2281 ctgccgcggc taccatggca ttggctgcac cttcttcacc cacccctgag tctcccacga
     2341 tgctgactaa gaagcccgag gataaccaca cccagtgcca gctggtgcct gtggtggaga
     2401 tcggcatcag tgagcgccag aatgctgagc agcatgtcac ccagctcatg tccaccgagc
     2461 ctctgccccg cactctgtcc ccgactccag cttcagccac agctccaacc tcccagggca
     2521 tcccgacatc agatgaggaa tcaacaccta agaagtcgaa ggccaggata ctggagtcca
     2581 tcacagtgag ctccctgatg gctacacccc aggaccccaa gggtcaaggg gtcgggacgg
     2641 ggcggtctgc gtctaagctc aggattgcgc aggtctcccc tggcccacgg gactcgccag
     2701 cctttccaga ttctccatgg cgggagcggg tgctggctcc catcctgccg gataacttct
     2761 ccacgcccac gggctctcgc acggactctc aatcggtgcg gcacagcccg atcgccccgt
     2821 cttccccgag tccccgagtc ttagcccaga agtactctct ggtggccaaa caggaaagtg
     2881 ttgtccgcag ggcgagcaga aggcttgcca agaagactgc cgaagagcca gctgcctctg
     2941 gccgcatcat ctgtcacagt tacctggaga ggctcctgaa tgttgaggtg ccccagaaag
     3001 ttggttctga gcagaaggaa ccccccgagg aggctgagcc tgtggcggca gctgagccag
     3061 aggtccctga gaacaacgga aataactcgt ggccccacaa tgacacagag atcgccaaca
     3121 gcacacccaa cccgaagcct gcagccagca gcccggaaac accctctgca gggcagcaag
     3181 aggccaagac ggaccaagca gatggaccca gagagccacc gcagagtgcc aggaggaagc
     3241 gcagctacaa gcaggccgtg agtgagctgg acgaggagca gcacctggag gatgaggagc
     3301 tgcagccccc caggagcaag accccttcct caccctgccc agccagcaag gtggtacggc
     3361 ccctccggac ctttctgcac acagtgcaga ggaaccagat gctcacgacc ccgacctcag
     3421 ccccacgcag cgtcatgaag tcctttatta agcgcaacac tcccctgcgc atggacccca
     3481 agtgcagctt cgtcgagaag gagcggcagc gcctggagaa tctgcggcgg aaggaggagg
     3541 ccgagcagct gcgcaggcag aaggtggagg aggacaagcg gcggcggctg gaggaggtga
     3601 agctgaagcg tgaggaacgc ctccgcaagg tgctgcaggc ccgcgagcgg gtggagcaga
     3661 tgaaggagga gaagaagaag cagattgagc agaagtttgc tcagatcgac gagaagactg
     3721 agaaggccaa ggaggagcgg ctggcagagg agaaggccaa gaaaaaggcg gcggccaaga
     3781 agatggagga ggtggaagca cgcaggaagc aggaagagga tgcacgtagg ctcaggtggc
     3841 tgcagcagga ggaggaagag cggcggcacc aagagctgct gcagaagaag aaggaagagg
     3901 agcaggagcg gctgcggaag gcggccgagg ctaagcggct ggcagagcag cgggagcagg
     3961 agcggcggga gcaggagcgg cgcgagcagg agcggcgcga gcaggagcgg cgggagcagg
     4021 agcggcgcga gcaggagcga cagctggcag agcaggagcg tcggcgggag caggagcggc
     4081 tccaggccga gagggagctg caggagcggg agaaggccct gcggctgcag aaggagcagc
     4141 tgcagaggga actggaggag aagaaaaaga aggaagagca gcagcgtctg gctgagcggc
     4201 agctgcagga ggagcaagag aagaaagcca aggaggcagc aggggccagc aaggccctga
     4261 atgtgactgt ggacgtgcag tctccagctt gtacctcata tcagatgact ccgcaagggc
     4321 acagggcccc tcccaagatc aacccagata actacgggat ggatctgaat agcgacgact
     4381 ccaccgatga tgaggcccat ccccggaagc ccatccccac ctgggcccga ggcaccccgc
     4441 tcagccaggc tatcattcac cagtactacc acccaccgaa ccttctggag ctctttggaa
     4501 ccattctccc actggacttg gaggatatct tcaagaagag caagccccgc tatcacaagc
     4561 gcaccagctc tgctgtctgg aactcaccgc ccctgcaggg cgccagggtc cccagcagcc
     4621 tggcctacag cctgaagaag cacaagcttc gaattctgca gtcgacggta ccgcgggccc
     4681 gggatccaat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc
     4741 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
     4801 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
     4861 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
     4921 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
     4981 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc
     5041 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
     5101 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
     5161 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
     5221 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
     5281 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
     5341 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaaa
     5401 gcggccgcga ctctagaggg ccctattcta tagtgtcacc taaatgctag agctcgctga
     5461 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     5521 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     5581 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     5641 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     5701 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     5761 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     5821 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     5881 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     5941 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     6001 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     6061 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     6121 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     6181 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     6241 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     6301 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     6361 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     6421 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     6481 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     6541 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     6601 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     6661 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     6721 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     6781 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     6841 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     6901 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     6961 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     7021 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     7081 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     7141 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     7201 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     7261 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     7321 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     7381 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     7441 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     7501 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     7561 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     7621 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     7681 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     7741 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     7801 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     7861 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     7921 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     7981 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     8041 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8101 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8161 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8221 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     8281 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     8341 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     8401 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     8461 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     8521 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     8581 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     8641 caccgctggt agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     8701 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     8761 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     8821 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     8881 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     8941 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     9001 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     9061 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     9121 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     9181 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     9241 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     9301 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     9361 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     9421 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     9481 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     9541 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     9601 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     9661 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     9721 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     9781 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     9841 cacatttccc cgaaaagtgc cacctgacgt c
//
