LOCUS       sequence-84493-e        9283 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Target INCENP to centromeres via CENP-B DNA binding domain (DBD).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9283)
  AUTHORS   Wang E, Ballister ER, Lampson MA
  TITLE     Aurora B dynamics at centromeres create a diffusion-based 
            phosphorylation gradient.
  JOURNAL   J Cell Biol. 2011 Aug 22;194(4):539-49. doi: 10.1083/jcb.201103044. 
            Epub 2011 Aug 15.
  PUBMED    21844210
REFERENCE   2  (bases 1 to 9283)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201103044"; journalName: "J Cell Biol"; date: 
            "2011-08-22- 22"; volume: "194"; issue: "4"; pages: "539-49"
COMMENT     Sequence Label: Cenp-B DBD INCENP GFP
FEATURES             Location/Qualifiers
     source          1..9283
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      903..912
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4086..4095
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4092..4094,4095..4097,4098..4811)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4092..4094,4095..4097,4098..4808)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4137..4158)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4398..4417)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4745..4766
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(4839..4857)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4840..4857)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4877..4894)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4883..5107
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5153..5581
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5240..5259)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5450..5471
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5590..5610)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5595..5924
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5775..5910
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5837..5856
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5991..6785
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6045..6064)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6655..6674
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6959..7080
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6996..7015)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7050..7069
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7129..7145)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7129..7145)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7142..7164)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7153..7169
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7177..7183,7184..7201,7202..7207))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7222..7243
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7360..7377)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7531..8116)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7611..7630)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8287..9078,9079..9147))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8910..8929
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9148..9252)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggcgcg
      901 ccgccaccat gggccccaag aggcgacagc tgacgttccg ggagaagtca cggatcatcc
      961 aggaggtgga ggagaatccg gacctgcgca agggcgagat cgcgcggcgc ttcaacatcc
     1021 cgccgtccac gctgagcacg atcctgaaga acaagcgcgc catcctggcg tcggagcgca
     1081 agtacggggt ggcctccacc tgccgcaaga ccaacaagct gtctccctac gacaagctcg
     1141 agggcttgct catcgcctgg ttccagcaga tccgcgccgc cggcctgccg gtcaagggca
     1201 tcatcctcaa ggagaaggcg ctgcgcatag ccgaggagct gggcatggac gacttcaccg
     1261 cctccaacgg ctggctggac cgcttccgcc ggcgccacgg cgtggtgtcc tgcagcggcg
     1321 tggcccgcgc ccgcgcgcga aacgctgccc cccgcacccc ggcggcgcct gccagtccgg
     1381 ccgcggtgcc ctcggagggc agtggcgggc tagcagggag cactgaattc agcaaagagc
     1441 cagagctgat gcccaaaaca ccttctcaga agaaccgacg gaagaagaga cggatttctt
     1501 atgttcagga tgaaaacaga gatcccatca ggagaaggtt atcccgcaga aagtctcgga
     1561 gcagccagct gagctcccga cgcctccgca gcaaggacag tgtagagaag ctggctacag
     1621 tggtcgggga gaacggctcc gttctgcggc gtgtgacccg tgctgcggct gcagctgccg
     1681 cggctaccat ggcattggct gcaccttctt cacccacccc tgagtctccc acgatgctga
     1741 ctaagaagcc cgaggataac cacacccagt gccagctggt gcctgtggtg gagatcggca
     1801 tcagtgagcg ccagaatgct gagcagcatg tcacccagct catgtccacc gagcctctgc
     1861 cccgcactct gtccccgact ccagcttcag ccacagctcc aacctcccag ggcatcccga
     1921 catcagatga ggaatcaaca cctaagaagt cgaaggccag gatactggag tccatcacag
     1981 tgagctccct gatggctaca ccccaggacc ccaagggtca aggggtcggg acggggcggt
     2041 ctgcgtctaa gctcaggatt gcgcaggtct cccctggccc acgggactcg ccagcctttc
     2101 cagattctcc atggcgggag cgggtgctgg ctcccatcct gccggataac ttctccacgc
     2161 ccacgggctc tcgcacggac tctcaatcgg tgcggcacag cccgatcgcc ccgtcttccc
     2221 cgagtccccg agtcttagcc cagaagtact ctctggtggc caaacaggaa agtgttgtcc
     2281 gcagggcgag cagaaggctt gccaagaaga ctgccgaaga gccagctgcc tctggccgca
     2341 tcatctgtca cagttacctg gagaggctcc tgaatgttga ggtgccccag aaagttggtt
     2401 ctgagcagaa ggaacccccc gaggaggctg agcctgtggc ggcagctgag ccagaggtcc
     2461 ctgagaacaa cggaaataac tcgtggcccc acaatgacac agagatcgcc aacagcacac
     2521 ccaacccgaa gcctgcagcc agcagcccgg aaacaccctc tgcagggcag caagaggcca
     2581 agacggacca agcagatgga cccagagagc caccgcagag tgccaggagg aagcgcagct
     2641 acaagcaggc cgtgagtgag ctggacgagg agcagcacct ggaggatgag gagctgcagc
     2701 cccccaggag caagacccct tcctcaccct gcccagccag caaggtggta cggcccctcc
     2761 ggacctttct gcacacagtg cagaggaacc agatgctcac gaccccgacc tcagccccac
     2821 gcagcgtcat gaagtccttt attaagcgca acactcccct gcgcatggac cccaagtgca
     2881 gcttcgtcga gaaggagcgg cagcgcctgg agaatctgcg gcggaaggag gaggccgagc
     2941 agctgcgcag gcagaaggtg gaggaggaca agcggcggcg gctggaggag gtgaagctga
     3001 agcgtgagga acgcctccgc aaggtgctgc aggcccgcga gcgggtggag cagatgaagg
     3061 aggagaagaa gaagcagatt gagcagaagt ttgctcagat cgacgagaag actgagaagg
     3121 ccaaggagga gcggctggca gaggagaagg ccaagaaaaa ggcggcggcc aagaagatgg
     3181 aggaggtgga agcacgcagg aagcaggaag aggatgcacg taggctcagg tggctgcagc
     3241 aggaggagga agagcggcgg caccaagagc tgctgcagaa gaagaaggaa gaggagcagg
     3301 agcggctgcg gaaggcggcc gaggctaagc ggctggcaga gcagcgggag caggagcggc
     3361 gggagcagga gcggcgcgag caggagcggc gcgagcagga gcggcgggag caggagcggc
     3421 gcgagcagga gcgacagctg gcagagcagg agcgtcggcg ggagcaggag cggctccagg
     3481 ccgagaggga gctgcaggag cgggagaagg ccctgcggct gcagaaggag cagctgcaga
     3541 gggaactgga ggagaagaag aaggaagagc agcagcgtct ggctgagcgg cagctgcagg
     3601 aggagcaaga gaagaaagcc aaggaggcag cacgggccag caaggccctg aatgtgactg
     3661 tggacgtgca gtctccagct tgtacctcat atcagatgac tccgcaaggg cacagggccc
     3721 ctcccaagat caacccagat aactacggga tggatctgaa tagcgacgac tccaccgatg
     3781 atgaggccca tccccggaag cccatcccca cctgggcccg aggcaccccg ctcagccagg
     3841 ctatcattca ccagtactac cacccaccga accttctgga gctctttgga accattctcc
     3901 cactggactt ggaggatatc ttcaagaaga gcaagccccg ctatcacaag cgcaccagct
     3961 ctgctgtctg gaactcaccg cccctgcagg gcgccagggt ccccagcagc ctggcctaca
     4021 gcctgaagaa gcacaagctt cgaattctgc agtcgacggt accgcgggcc cgggatccac
     4081 cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     4141 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     4201 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     4261 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     4321 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     4381 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     4441 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     4501 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     4561 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     4621 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     4681 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     4741 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     4801 tgtacaagta aagcggccgc gactctagag ggccctattc tatagtgtca cctaaatgct
     4861 agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     4921 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     4981 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     5041 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     5101 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     5161 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     5221 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     5281 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     5341 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     5401 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     5461 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     5521 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     5581 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     5641 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     5701 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     5761 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     5821 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     5881 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     5941 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
     6001 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     6061 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     6121 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     6181 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     6241 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     6301 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     6361 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     6421 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     6481 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     6541 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     6601 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     6661 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     6721 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     6781 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     6841 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     6901 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     6961 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     7021 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     7081 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     7141 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     7201 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     7261 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     7321 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     7381 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     7441 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     7501 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     7561 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     7621 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     7681 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     7741 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     7801 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     7861 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     7921 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     7981 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     8041 cggcaaacaa accaccgctg gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag
     8101 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     8161 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     8221 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     8281 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     8341 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     8401 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     8461 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     8521 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     8581 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     8641 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     8701 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     8761 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     8821 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     8881 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     8941 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     9001 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     9061 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     9121 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     9181 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     9241 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc
//
