LOCUS       sequence-84496-e       10582 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Target INCENP to centromeres via full length CENP-B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10582)
  AUTHORS   Wang E, Ballister ER, Lampson MA
  TITLE     Aurora B dynamics at centromeres create a diffusion-based 
            phosphorylation gradient.
  JOURNAL   J Cell Biol. 2011 Aug 22;194(4):539-49. doi: 10.1083/jcb.201103044. 
            Epub 2011 Aug 15.
  PUBMED    21844210
REFERENCE   2  (bases 1 to 10582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201103044"; journalName: "J Cell Biol"; date: 
            "2011-08-22- 22"; volume: "194"; issue: "4"; pages: "539-49"
COMMENT     Sequence Label: Cenp-B INCENP GFP
FEATURES             Location/Qualifiers
     source          1..10582
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      903..912
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      5385..5394
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5391..5393,5394..5396,5397..6110)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5391..5393,5394..5396,5397..6107)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5436..5457)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5697..5716)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6044..6065
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(6138..6156)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6139..6156)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6176..6193)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6182..6406
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6452..6880
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6539..6558)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6749..6770
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6889..6909)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6894..7223
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7074..7209
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7136..7155
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7290..8084
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7344..7363)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7954..7973
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8258..8379
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8295..8314)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8349..8368
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8428..8444)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8428..8444)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8441..8463)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8452..8468
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8476..8482,8483..8500,8501..8506))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8521..8542
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8659..8676)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8830..9415)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8910..8929)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9586..10377,10378..10446))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10209..10228
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10447..10551)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggcgcg
      901 ccgccaccat gggccccaag aggcgacagc tgacgttccg ggagaagtca cggatcatcc
      961 aggaggtgga ggagaatccg gacctgcgca agggcgagat cgcgcggcgc ttcaacatcc
     1021 cgccgtccac gctgagcacg atcctgaaga acaagcgcgc catcctggcg tcggagcgca
     1081 agtacggggt ggcctccacc tgccgcaaga ccaacaagct gtctccctac gacaagctcg
     1141 agggcttgct catcgcctgg ttccagcaga tccgcgccgc cggcctgccg gtcaagggca
     1201 tcatcctcaa ggagaaggcg ctgcgcatag ccgaggagct gggcatggac gacttcaccg
     1261 cctccaacgg ctggctggac cgcttccgcc ggcgccacgg cgtggtgtcc tgcagcggcg
     1321 tggcccgcgc ccgcgcgcga aacgctgccc cccgcacccc ggcggcgcct gccagtccgg
     1381 ccgcggtgcc ctcggagggc agtggcggga gcactactgg ttggcgcgct cgggaggagc
     1441 agccgccgtc ggtggccgag ggctacgcct cgcaggacgt gttcagcgcc accgagacca
     1501 gtctatggta cgacttcctg cccgaccagg ccgcggggct gtgcggaggc gacggacggc
     1561 cgcgtcaagc cacccagcgc ctgagcgtcc tgctatgcgc caatgccgac ggcagcgaga
     1621 agctgccccc gctggtggcc ggcaagtcgg ccaagccccg cgcaggccaa gccggcctgc
     1681 cctgcgacta caccgccaac tccaagggtg gtgtcaccac ccaggccctg gccaagtact
     1741 tgaaggcctt ggacacccga atggctgcag agtctcgccg ggtcctgctg ttggccggcc
     1801 gcttggctgc ccagtccttg gacacctcgg gcctgcggca tgtgcagctg gccttcttcc
     1861 ctcccggcac cgtgcatccg ctggagaggg gagtggtcca gcaggtgaag ggccactacc
     1921 gccaggccat gctgctcaag gccatggccg cgctagaggg ccaggatccc tcaggcctgc
     1981 agctgggtct cacggaggcc ctgcactttg tggctgccgc ctggcaggca gtggagcctt
     2041 cggacatagc cgcctgcttt cgtgaggctg gctttggggg tggccctaat gccaccatca
     2101 ccacttccct caagagtgag ggagaggaag aggaggagga ggaggaagaa gaggaggagg
     2161 aagagggtga aggagaggaa gaggaggagg aaggggagga ggaggaggag gaaggggggg
     2221 aaggagagga attgggggag gaagaggagg tggaggagga gggtgatgtt gatagtgatg
     2281 aagaagagga ggaagatgag gagagctcct cggagggctt ggaggctgag gactgggccc
     2341 agggagtagt ggaggccggt ggcagcttcg gggcttatgg tgcccaggag gaagcccagt
     2401 gccctactct gcatttcctg gaaggtgggg aggactctga ttcagacagt gaggaagagg
     2461 acgatgagga agaggatgat gaagatgaag acgacgatga tgatgaggag gatggtgatg
     2521 aggtgcctgt acccagcttt ggggaggcca tggcttactt tgccatggtc aagaggtacc
     2581 tgacctcctt ccccattgat gaccgcgtgc agagccacat cctccacttg gaacacgatc
     2641 tggttcatgt gaccaggaag aaccacgcca ggcaggcggg agttcgaggt cttggacatc
     2701 aaagcgggct agcagggagc actgaattca gcaaagagcc agagctgatg cccaaaacac
     2761 cttctcagaa gaaccgacgg aagaagagac ggatttctta tgttcaggat gaaaacagag
     2821 atcccatcag gagaaggtta tcccgcagaa agtctcggag cagccagcag agcacccgac
     2881 gcctccgcag caaggacagt gtagagaagc tggctacagt ggtcggggag aacggctccg
     2941 ttctgcggcg tgtgacccgt gctgcggctg cagctgccgc ggctaccatg gcattggctg
     3001 caccttcttc acccacccct gagtctccca cgatgctgac taagaagccc gaggataacc
     3061 acacccagtg ccagctggtg cctgtggtgg agatcggcat cagtgagcgc cagaatgctg
     3121 agcagcatgt cacccagctc atgtccaccg agcctctgcc ccgcactctg tccccgactc
     3181 cagcttcagc cacagctcca acctcccagg gcatcccgac atcagatgag gaatcaacac
     3241 ctaagaagtc gaaggccagg atactggagt ccatcacagt gagctccctg atggctacac
     3301 cccaggaccc caagggtcaa ggggtcggga cggggcggtc tgcgtctaag ctcaggattg
     3361 cgcaggtctc ccctggccca cgggactcgc cagcctttcc agattctcca tggcgggagc
     3421 gggtgctggc tcccatcctg ccggataact tctccacgcc cacgggctct cgcacggact
     3481 ctcaatcggt gcggcacagc ccgatcgccc cgtcttcccc gagtccccga gtcttagccc
     3541 agaagtactc tctggtggcc aaacaggaaa gtgttgtccg cagggcgagc agaaggcttg
     3601 ccaagaagac tgccgaagag ccagctgcct ctggccgcat catctgtcac agttacctgg
     3661 agaggctcct gaatgttgag gtgccccaga aagttggttc tgagcagaag gaaccccccg
     3721 aggaggctga gcctgtggcg gcagctgagc cagaggtccc tgagaacaac ggaaataact
     3781 cgtggcccca caatgacaca gagatcgcca acagcacacc caacccgaag cctgcagcca
     3841 gcagcccgga aacaccctct gcagggcagc aagaggccaa gacggaccaa gcagatggac
     3901 ccagagagcc accgcagagt gccaggagga agcgcagcta caagcaggcc gtgagtgagc
     3961 tggacgagga gcagcacctg gaggatgagg agctgcagcc ccccaggagc aagacccctt
     4021 cctcaccctg cccagccagc aaggtggtac ggcccctccg gacctttctg cacacagtgc
     4081 agaggaacca gatgctcacg accccgacct cagccccacg cagcgtcatg aagtccttta
     4141 ttaagcgcaa cactcccctg cgcatggacc ccaagtgcag cttcgtcgag aaggagcggc
     4201 agcgcctgga gaatctgcgg cggaaggagg aggccgagca gctgcgcagg cagaaggtgg
     4261 aggaggacaa gcggcggcgg ctggaggagg tgaagctgaa gcgtgaggaa cgcctccgca
     4321 aggtgctgca ggcccgcgag cgggtggagc agatgaagga ggagaagaag aagcagattg
     4381 agcagaagtt tgctcagatc gacgagaaga ctgagaaggc caaggaggag cggctggcag
     4441 aggagaaggc caagaaaaag gcggcggcca agaagatgga ggaggtggaa gcacgcagga
     4501 agcaggaaga ggatgcacgt aggctcaggt ggctgcagca ggaggaggaa gagcggcggc
     4561 accaagagct gctgcagaag aagaaggaag aggagcagga gcggctgcgg aaggcggccg
     4621 aggctaagcg gctggcagag cagcgggagc aggagcggcg ggagcaggag cggcgcgagc
     4681 aggagcggcg cgagcaggag cggcgggagc aggagcggcg cgagcaggag cgacagctgg
     4741 cagagcagga gcgtcggcgg gagcaggagc ggctccaggc cgagagggag ctgcaggagc
     4801 gggagaaggc cctgcggctg cagaaggagc agctgcagag ggaactggag gagaagaaga
     4861 aggaagagca gcagcgtctg gctgagcggc agctgcagga ggagcaagag aagaaagcca
     4921 aggaggcagc aggggccagc aaggccctga atgtgactgt ggacgtgcag tctccagctt
     4981 gtacctcata tcagatgact ccgcaagggc acagggcccc tcccaagatc aacccagata
     5041 actacgggat ggatctgaat agcgacgact ccaccgatga tgaggcccat ccccggaagc
     5101 ccatccccac ctgggcccga ggcaccccgc tcagccaggc tatcattcac cagtactacc
     5161 acccaccgaa ccttctggag ctctttggaa ccattctccc actggacttg gaggatatct
     5221 tcaagaagag caagccccgc tatcacaagc gcaccagctc tgctgtctgg aactcaccgc
     5281 ccctgcaggg cgccagggtc cccagcagcc tggcctacag cctgaagaag cacaagcttc
     5341 gaattctgca gtcgacggta ccgcgggccc gggatccacc ggtcgccacc atggtgagca
     5401 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     5461 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     5521 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     5581 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     5641 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     5701 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     5761 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     5821 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     5881 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     5941 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     6001 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     6061 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccgcg
     6121 actctagagg gccctattct atagtgtcac ctaaatgcta gagctcgctg atcagcctcg
     6181 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     6241 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     6301 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     6361 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     6421 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     6481 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     6541 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     6601 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     6661 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     6721 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     6781 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     6841 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     6901 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     6961 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     7021 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     7081 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     7141 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     7201 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     7261 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     7321 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     7381 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     7441 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     7501 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     7561 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     7621 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     7681 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     7741 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     7801 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     7861 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     7921 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     7981 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     8041 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     8101 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     8161 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     8221 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     8281 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     8341 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     8401 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     8461 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     8521 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     8581 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     8641 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     8701 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     8761 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     8821 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     8881 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     8941 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     9001 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     9061 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     9121 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     9181 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     9241 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     9301 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     9361 tagcggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     9421 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     9481 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     9541 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     9601 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     9661 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     9721 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     9781 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     9841 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     9901 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     9961 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    10021 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    10081 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    10141 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    10201 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    10261 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    10321 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    10381 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    10441 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    10501 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    10561 ccgaaaagtg ccacctgacg tc
//
