LOCUS       sequence-84505-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pcDNA3.0-based plasmid encoding fDHFR-UbK48R-A-beta-42 13myc under 
            the control of T7 or CMV promoter for 35S-pulse-chase URT-based 
            assays in rabbit reticulocyte extract..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Brower CS, Piatkov KI, Varshavsky A
  TITLE     Neurodegeneration-associated protein fragments as short-lived 
            substrates of the N-end rule pathway.
  JOURNAL   Mol Cell. 2013 Apr 25;50(2):161-71. doi: 
            10.1016/j.molcel.2013.02.009. Epub 2013 Mar 14.
  PUBMED    23499006
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2013.02.009"; journalName: "Mol Cell"; date: 
            "2013-04-25- 25"; volume: "50"; issue: "2"; pages: "161-71"
COMMENT     Sequence Label: pCB268
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             88..111
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             121..681
                     /codon_start=1
                     /gene="Mus musculus Dhfr"
                     /product="mouse dihydrofolate reductase"
                     /label=DHFR
                     /translation="MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVE
                     GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
                     ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
                     PGVLSEVQEEKGIKYKFEVYEKKD"
     CDS             688..714
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttatggac tacaaggacg acgatgacaa gggtggatcc
      121 atggttcgac cattgaactg catcgtcgcc gtgtcccaaa atatggggat tggcaagaac
      181 ggagacctac cctggcctcc gctcaggaac gagttcaagt acttccaaag aatgaccaca
      241 acctcttcag tggaaggtaa acagaatctg gtgattatgg gtaggaaaac ctggttctcc
      301 attcctgaga agaatcgacc tttaaaggac agaattaata tagttctcag tagagaactc
      361 aaagaaccac cacgaggagc tcattttctt gccaaaagtt tggatgatgc cttaagactt
      421 attgaacaac cggaattggc aagtaaagta gacatggttt ggatagtcgg aggcagttct
      481 gtttaccagg aagccatgaa tcaaccaggc cacctcagac tctttgtgac aaggatcatg
      541 caggaatttg aaagtgacac gtttttccca gaaattgatt tggggaaata taaacttctc
      601 ccagaatacc caggcgtcct ctctgaggtc caggaggaaa aaggcatcaa gtataagttt
      661 gaagtctacg agaagaaaga cggtacctac ccatacgatg tcccagatta cgccgctttc
      721 cttgcgggta gtgggtcgac catgcagatt ttcgtcaaga ctttgaccgg taaaaccata
      781 acattggaag ttgaatcttc cgataccatc gacaacgtta agtcgaaaat tcaagacaag
      841 gaagg
//
