LOCUS       sequence-84509-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express Cup9p‐FLAG from the PGAL1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Byrd C, Turner GC, Varshavsky A
  TITLE     The N-end rule pathway controls the import of peptides through 
            degradation of a transcriptional repressor.
  JOURNAL   EMBO J. 1998 Jan 2. 17(1):269-77.
  PUBMED    9427760
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            1998 Jan 2. 17(1):269-77."
COMMENT     Sequence Label: pCB116
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..26
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8..26
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        50..491
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    50..167
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     462..485
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     506..522
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     506..522
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctctagtac ggattagaag
       61 ccgccgagcg ggtgacagcc ctccgaagga agactctcct ccgtgcgtcc tcgtcttcac
      121 cggtcgcgtt cctgaaacgc agatgtgcct cgcgccgcac tgctccgaac aataaagatt
      181 ctacaatact agcttttatg gttatgaaga ggaaaaattg gcagtaacct ggccccacaa
      241 accttcaaat gaacgaatca aattaacaac cataggatga taatgcgatt agttttttag
      301 ccttatttct ggggtaatta atcagcgaag cgatgatttt tgatctatta acagatatat
      361 aaatgcaaaa actgcataac cactttaact aatactttca acattttcgg tttgtattac
      421 ttcttattca aatgtaataa aagtatcaac aaaaaattgt taatatacct ctatacttta
      481 acgtcaagga gaaaaaaccc cggattctag aactagtgga tccgaatagt tacattcgaa
      541 gatgaattat aactgcgaaa tacaaaacag gaacagtaag aatgttgaca atcaagtcag
      601 tctaccccct atccaagttc tatttaactc catagaaaaa cggagtatgc ccgagttggc
      661 attttcgaac atagaatact cgcatgggaa ccttcggtct agtaccgagg aacaaaacta
      721 tcctgcgcct gtgttacttc cacagcatca ttcgattgcg tatcccgcaa ttaattctgg
      781 cggtactagt actactgcta ctcctactgc ttctacagtt gaaacgtcca agacgagtag
      841 cagtgctatg gatacacaat ctcaatatgg cagttcaaag aagtcaaaat cggcgtccga
      901 tgatgcaaag ccctgctac
//
