LOCUS       sequence-84513-e         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter control for pMPRA3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Melnikov A, Zhang X, Rogov P, Wang L, Mikkelsen TS
  TITLE     Massively parallel reporter assays in cultured Mammalian cells.
  JOURNAL   J Vis Exp. 2014 Aug 17;(90). doi: 10.3791/51719.
  PUBMED    25177895
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Vis 
            Exp."; date: "2014-08-17"; volume: "(90). doi"; pages: " 
            10.3791/51719"
COMMENT     Sequence Label: pMPRA3-CMV-Clover
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(120..122,123..125,126..836)
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from GFP (Lam et al., 2012)"
                     /label=Clover
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(165..186)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     773..794
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(852..870)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(853..870)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(939..958)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt accgagctcg gatccactag taacggccgc cagtgtgctg gaattcggca
      121 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
      181 gcgacgtaaa cggccacaag ttcagcgtcc gcggcgaggg cgagggcgat gccaccaacg
      241 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
      301 tcgtgaccac cttcggctac ggcgtggcct gcttcagccg ctaccccgac cacatgaagc
      361 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatctctt
      421 tcaaggacga cggtacctac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
      481 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
      541 agctggagta caacttcaac agccacaacg tctatatcac ggccgacaag cagaagaacg
      601 gcatcaaggc taacttcaag atccgccaca acgttgagga cggcagcgtg cagctcgccg
      661 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
      721 acctgagcca tcagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
      781 tgctggagtt cgtgaccgcc gccgggatta cacatggcat ggacgagctg tacaagtcta
      841 gagggcccta ttctatagtg tcacctaaat gctagggcct cggcggccaa gctagtcggg
      901 gcggccggcc gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa
      961 ctagaatgca gtgaaa
//
