LOCUS       sequence-84516-e         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses human myc-14-3-3ϵ in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Chen HK, Fernandez-Funez P, Acevedo SF, Lam YC, Kaytor MD, Fernandez
            MH, Aitken A, Skoulakis EM, Orr HT, Botas J, Zoghbi HY
  TITLE     Interaction of Akt-phosphorylated ataxin-1 with 14-3-3 mediates 
            neurodegeneration in spinocerebellar ataxia type 1.
  JOURNAL   Cell. 2003 May 16;113(4):457-68.
  PUBMED    12757707
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2003-05-16"; volume: "113(4)"; pages: "457-68"
COMMENT     Sequence Label: pcDNA3.1-HA-14-3-3 ϵ
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    248..369
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(285..304)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     339..358
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    453..915
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(613..632)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     842..859
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
       61 ttccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
      121 ctattacgcc agcccggatc gatccttatc ggattttacc acatttgtag aggttttact
      181 tgctttaaaa aacctcccac atctccccct gaacctgaaa cataaaatga atgcaattgt
      241 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
      301 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
      361 tgtatcttat catgtctgct cgaagcatta accctcacta aagggaagcg gccgcccggg
      421 tcgactctag aggatcccgg gttgtggcaa gcttatcatc gtgtttttca aaggaaaacc
      481 acgtccccgt ggttcggggg gcctagacgt ttttttaacc tcgactaaac acatgtaaag
      541 catgtgcacc gaggccccag atcagatccc atacaatggg gtaccttctg ggcatccttc
      601 agccccttgt tgaatacgct tgaggagagc catttgactc tttccacaac tatccaactc
      661 acaacgtggc actggggttg tgccgccttt gcaggtgtat cttatacacg tggcttttgg
      721 ccgcagaggc acctgtcgcc aggtgggggg ttccgctgcc tgcaaagggt cgctacagac
      781 gttgtttgtc ttcaagaagc ttccagagga actgcttcct tcacgacatt caacagacct
      841 tgcattcctt tggcgagagg ggaaagaccc ctaggaatgc tcgtcaagaa gacagggcca
      901 ggtttccggg ccctcacatt gccaaaagac ggcaatatgg tggaa
//
