LOCUS       sequence-84519-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses alpha hemolysin under the control of theophylline 
            riboswitch.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Lentini R, Santero SP, Chizzolini F, Cecchi D, Fontana J, 
            Marchioretto M, Del Bianco C, Terrell JL, Spencer AC, Martini L, 
            Forlin M, Assfalg M, Dalla Serra M, Bentley WE, Mansy SS
  TITLE     Integrating artificial with natural cells to translate chemical 
            messages that direct E. coli behaviour.
  JOURNAL   Nat Commun. 2014 May 30;5:4012. doi: 10.1038/ncomms5012.
  PUBMED    24874202
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-05-30"; volume: "5:4012. doi"; pages: " 
            10.1038/ncomms5012"
COMMENT     Sequence Label: JF001A
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac
       61 tcactatagg gtgataccag catcgtcttg atgcccttgg cagcaccctg ctaaggtaac
      121 aacaagatgg attctgatat caatatcaaa accggcacca ccgatatcgg ctccaatacc
      181 accgttaaaa ccggtgatct ggtgacctat gataaagaaa acggtatgca taaaaaagtg
      241 ttttactcgt ttattgacga taaaaaccat aacaaaaaac tgctggtcat ccgcaccaaa
      301 ggcaccattg cgggtcaata ccgtgtgtac tccgaagaag gtgcgaacaa aagcggtctg
      361 gcttggccgt ctgcctttaa agtgcagctg caactgccgg ataatgaagt ggcgcagatt
      421 tcagattatt atccgcgtaa tagcatcgat accaaagaat atatgagtac cctgacctat
      481 ggttttaatg gcaatgttac cggtgatgat acgggtaaaa ttggcggtct gattggcgcc
      541 aatgtgtcca ttggtcatac gctgaaatac gtgcaaccgg atttcaaaac cattctggaa
      601 agtccgaccg ataaaaaagt gggttggaaa gttatcttca acaacatggt gaatcagaac
      661 tggggtccgt acgatcgcga ttcctggaat ccggtttatg gcaatcagct gtttatgaaa
      721 acccgcaacg gtagtatgaa agcggcggat aattttctgg acccgaacaa agcctcaagc
      781 ctgctgtcca gcggttttag cccggatttt gccacggtta ttaccatgga tcgcaaagcc
      841 agcaaacagc agaccaacat tgatgtgatc tacgaacgtg
//
